A5078002615- Prion Diseases and Protein Misfolding
- Toxoplasma gondii Research Studies
- Herpesvirus Infections and Treatments
- Animal Disease Management and Epidemiology
- Parasitic Infections and Diagnostics
- Vector-borne infectious diseases
- Viral Infections and Vectors
- Neurological diseases and metabolism
- Microbial infections and disease research
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Virus-based gene therapy research
- Trace Elements in Health
- Viral gastroenteritis research and epidemiology
- T-cell and Retrovirus Studies
- Tendon Structure and Treatment
- Bacillus and Francisella bacterial research
- HIV Research and Treatment
- Vector-Borne Animal Diseases
- Coccidia and coccidiosis research
- Toxin Mechanisms and Immunotoxins
- Bacteriophages and microbial interactions
- Veterinary Equine Medical Research
- Infectious Encephalopathies and Encephalitis
- Chemical Safety and Risk Management
Washington State University
2014-2023
University of Washington
2022
Washington State Department of Health
2022
Public Health – Seattle & King County
2022
University of Idaho
2020
University of Kentucky
2020
University of Wyoming
2020
University of Guelph
2020
Washington State Department of Agriculture
2010-2015
Ames National Laboratory
2015
This document is the consensus of American Association Veterinary Laboratory Diagnosticians (AAVLD) Subcommittee on Standardization Immunohistochemistry a set guidelines for immunohistochemistry (IHC) testing in veterinary laboratories. powerful ancillary methodology frequently used many laboratories both diagnostic and research purposes. However, neither standardization nor validation IHC tests has been completely achieved medicine. addresses issues. Topics covered include antibody...
Novel Eurasian lineage avian influenza A(H5N8) virus has spread rapidly and globally since January 2014. In December 2014, H5N8 reassortant H5N2 viruses were detected in wild birds Washington, USA, subsequently backyard birds. When they infect commercial poultry, these highly pathogenic pose substantial trade issues.
Ovine scrapie is a member of the transmissible spongiform encephalopathies (TSEs), heterogeneous family fatal neurologic disorders characterized by deposition an abnormal isoform (prion protein [PrP] PrP-Sc) cellular sialoglycoprotein in neural tissue. PrP-Sc detectable some lymphoid tissues infected sheep months or years before development clinical disease. Detection these basis for live-animal testing. In this study, we characterize performance preclinical diagnostic test ovine based on...
ABSTRACT Eleven laboratories collaborated to determine the periodic prevalence of Salmonella in a population dogs and cats United States visiting veterinary clinics. Fecal samples (2,965) solicited from 11 geographically dispersed testing were collected 36 states between January 2012 April 2014 tested using harmonized method. The overall study (3 542) was <1%. (60 2,422) 2.5%. Diarrhea present only 55% positive dogs; however, 3.8% all diarrheic positive, compared with 1.8% nondiarrheic...
ABSTRACT The transmissible spongiform encephalopathies are a heterogeneous group of fatal neurodegenerative disorders occurring in humans, mink, cats, and ruminant herbivores. occurrence novel cattle the United Kingdom Europe mule deer elk parts States has emphasized need for reliable diagnostic tests with standardized reagents. Postmortem diagnosis is performed by histologic examination brain sections from affected animals. histopathological criteria include gliosis, astrocytosis, neuronal...
The breadth of animal hosts that are susceptible to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and may serve as reservoirs for continued viral transmission not known entirely. In August 2020, an outbreak SARS-CoV-2 occurred on five mink farms in Utah was associated with high mortality (35–55% adult mink) rapid between animals. premise clinical disease information, pathology, molecular characterization, tissue distribution virus within infected during the early phase...
Neospora caninum, an apicomplexan parasite closely related to Toxoplasma gondii, causes abortion, stillbirths, and congenital neurologic disease in multiple animal species. The present study focuses on the development of encephalitis intracerebral load that occurs 6 wk postinfection (PI). Utilizing BALB/c, C57BL/6, B10.D2 mice, initial investigation was undertaken determine relative resistance inbred strains N. caninum-induced encephalitis. Relative defined terms central nervous system...
ABSTRACT The routine diagnosis of Neospora caninum abortion is based upon histopathologic changes in fetal tissues and identification tissue parasites by immunohistochemistry. Confirmation N. infection immunohistochemistry has low sensitivity. In the present study, we examined utility PCR detecting from spontaneous bovine abortion. DNA was obtained fresh formalin-fixed 61 fetuses submitted for diagnosis. Histopathology determined true status each fetus. paraffin-embedded tissues, detected 13...
From 2002 to 2007, 23 ferrets from Europe and the United States were diagnosed with systemic pyogranulomatous inflammation resembling feline infectious peritonitis (FIP). The average age at time of diagnosis was 11 months. disease progressive in all cases, duration clinical illness 67 days. Common findings anorexia, weight loss, diarrhea, large, palpable intra-abdominal masses; less frequent included hind limb paresis, central nervous system signs, vomiting, dyspnea. Frequent hematologic...
Abstract SARS-CoV-2 likely emerged from an animal reservoir. However, the frequency of and risk factors for interspecies transmission remain unclear. We conducted a community-based study in Idaho, USA, pets households that had >1 confirmed infections humans. Among 119 dogs 57 cats, clinical signs consistent with were reported 20 (21%) 19 cats (39%). Of 81 32 sampled, 40% 43% seropositive, 5% 8% PCR positive. This discordance might be caused by delays sampling. Respondents commonly close...
ABSTRACT A competitive-inhibition enzyme-linked immunosorbent assay (cELISA) was developed for detection of equine antibodies specific Babesia caballi . The used recombinant B. rhoptry-associated protein 1 (RAP-1) and monoclonal antibody (MAb) 79/17.18.5, which is reactive with a peptide epitope native 60-kDa antigen. gene encoding the antigen sequenced, database analysis revealed that product protein. Cloning expression truncated copy demonstrated MAb 79/17.18.5 reacts C-terminal repeat...
ABSTRACT The relative fitness of arthropod-borne pathogens within the vector can be a major determinant pathogen prevalence mammalian host population. Strains tick-borne rickettsia Anaplasma marginale differ markedly in transmission efficiency, with consequent impact on strain structure. We have identified two A. strains significant differences phenotype that is effected following infection salivary gland. proposed competing hypotheses to explain phenotypes: (i) both are secreted equally,...
ABSTRACT A previously described monoclonal antibody (MAb)-based competitive-inhibition enzyme-linked immunosorbent assay (cELISA) was modified to optimize performance, and the validated in various defined cattle populations for detection of serum Neospora caninum , a major cause bovine abortion. Modifications cELISA included capturing native N. antigen with parasite-specific MAb (MAb 5B6-25) directly conjugating competitor 4A4-2), both MAbs binding different epitopes conserved,...
Bacterial pathogens in the genera Anaplasma and Ehrlichia encode a protein superfamily, pfam01617, which includes predominant outer membrane proteins (OMPs) of each species, major surface 2 (MSP2) MSP3 marginale ovis, phagocytophilum MSP2 (p44), chaffeensis p28-OMP, canis p30, ruminantium MAP1, has been shown to be involved both antigenic variation within mammalian host differential expression between arthropod hosts. Recently, complete sequencing A. genome identified an expanded set genes,...
ABSTRACT A pregnant sea lion stranded in the State of Washington was found to have placentitis caused by a unique strain Coxiella burnetii . This is first description coxiellosis and suggests that exposure lions may be risk factor for contracting Q fever.
Abstract IL-4 levels were modulated in mice to test the hypothesis that induction of a maternal type 1 response would decrease frequency congenital Neospora caninum transmission. This tested relationship between and both innate adaptive immunity utilizing two basic experimental designs. In first, was neutralized with mAb during pregnancy naive concomitant initial, virulent infection. second, before priming inoculation consisting live, avirulent N. tachyzoites followed by challenge subsequent...
The suitability of mice as a model for reproductive loss due to Neospora caninum infection was investigated. Groups were infected with 2 x 10(6) N. before pregnancy (10 days) and during (days 5 10 gestation). In early pregnancy, fetal evaluated throughout gestation, by enumeration resorptions total fetuses. no difference present in between control mice, although litter size decreased the (P < 0.05). (day gestation) examined temporally increased compared at days gestation one time point 14...