A5057165114- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Animal Genetics and Reproduction
- Reproductive System and Pregnancy
- Mitochondrial Function and Pathology
- Molecular Biology Techniques and Applications
- Aquaculture Nutrition and Growth
- Physiological and biochemical adaptations
- Reproductive Biology and Fertility
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Receptor Mechanisms and Signaling
- Reproductive biology and impacts on aquatic species
- Drug Transport and Resistance Mechanisms
- Estrogen and related hormone effects
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- Extracellular vesicles in disease
- Nuclear Structure and Function
- Epigenetics and DNA Methylation
- Circular RNAs in diseases
- Autophagy in Disease and Therapy
- Animal health and immunology
- Steroid Chemistry and Biochemistry
- Neurobiology and Insect Physiology Research
- Caveolin-1 and cellular processes
Cornell University
2018-2025
New York State College of Agriculture & Life Sciences
2018-2023
West Virginia University
2016-2020
Neurosteroids synthesized within the central nervous system modulate neurotransmission, enhance neuroprotection, regulate immune responses, and influence cognitive behavioral processes. Beyond de novo synthesis of pregnenolone (PREG) from cholesterol, brain also engages in intermediary synthesis, converting local or circulating precursors into active neurosteroids. However, specific cell types regions involved remain poorly defined. In this study, we used single-cell transcriptomic data to...
Neurosteroids synthesized within the central nervous system play essential roles in modulating neurotransmission, providing neuroprotection, regulating immune responses, influencing behavior and cognition, mediating stress physiology. Despite their broad significance, specific brain cell types capable of de novo steroid synthesis from cholesterol remain poorly defined. In this study, we analyzed single-cell transcriptomic data to map steroidogenic gene expression across populations murine...
The import of cholesterol to the inner mitochondrial membrane by steroidogenic acute regulatory protein (STAR/STARD1) is essential for de novo steroid hormone biosynthesis and acidic pathway bile acid synthesis. This robust system, evolved start stop colossal movement, ensures pulsatile yet swift metabolism in cells. Nonetheless, proposed mechanism components involved this process has remained a topic ongoing debate. In study, we elucidate machinery structural aspects STAR, revealing its...
Although derivation of naïve bovine embryonic stem cells is unachieved, the possibility for generation induced pluripotent (biPSCs) has been generally reported. However, attempts to sustain biPSCs by promoting self-renewal have not successful. Methods established maintaining murine and human (iPSCs) do support iPSCs any bovid species. In this study, we examined methods enhance complete reprogramming concurrently investigated signaling relevant pluripotency blastocyst inner cell mass (ICM)....
Abstract Background Long non-coding RNAs (lncRNAs) are key regulators of diverse cellular processes. Although a number studies have reported the identification bovine lncRNAs across many tissues, very little is known about identity and characteristics in oocytes. Methods A oocyte cDNA library was constructed sequenced using Illumina HiSeq 2000 sequencing system. The transcriptome ab initio assembly software Scripture Cufflinks. assembled transcripts were categorized to identify novel...
Estradiol (E2) is a steroid hormone that negatively affects muscle growth in rainbow trout (Oncorhynchus mykiss), but the mechanisms directing with this response are not fully understood. To better characterize effects of E2 muscle, we identified differentially regulated mRNAs and lncRNAs juvenile exposed to E2. Here, performed next-generation RNA sequencing comprehensive bioinformatics analyses transcriptome profiles, including long noncoding RNAs (lncRNAs), skeletal injected A total 226...
In Leydig cells, intrinsic factors that determine cellular steroidogenic efficiency is of functional interest to decipher and monitor pathophysiology in many contexts. Nevertheless, beyond basic regulation cholesterol storage mobilization, systems biology interpretation the metabolite networks function deficient. To reconstruct describe different molecular regulating steroidogenesis, we profiled metabolites resting MA-10 cells. Our results identified 283-annotated components (82 neutral...
Lhx8 is an important transcription factor that preferentially expressed in germ cells. null mice are infertile due to lack of oocytes and impairment the transition from primordial follicles primary follicles. deficiency also affects expression many oocyte-specific genes. In this study, we report characterization rainbow trout lhx8 genes identification a novel cell-specific nuclear interacts with Lhx8. Two genes, lhx8a lhx8b, were identified, encoding proteins 344 361 amino acids,...
Abstract Th17 cells are critical drivers of autoimmune diseases and immunopathology. There is an unmet need to develop therapies targeting pathogenic for the treatment disorders. Here, we report that anxiolytic FGIN-1-27 inhibits differentiation pathogenicity in vitro vivo using experimental encephalomyelitis (EAE) model cell-driven pathology. Remarkably, found effects were independent translocator protein (TSPO), reported target this small molecule, instead driven by a metabolic switch led...
Despite being a highly conserved protein, the precise role of mitochondrial translocator protein (TSPO), previously known as peripheral benzodiazepine receptor (PBR), remains elusive. The void created by studies that overturned presumptive model described TSPO/PBR cholesterol transporter for steroidogenesis has been filled with evidence it can affect metabolic functions across different systems. We reported deficient steroidogenic cells upregulate fatty acid oxidation and presented strong...
MA-10 cells, established 4 decades ago from a murine Leydig cell tumor, has served as key model system for studying steroidogenesis. Despite precipitous loss in their innate ability to respond luteinizing hormone (LH), the use of cell-permeable cAMP analog induction ensured continued use. In parallel, paradigm that serum-free conditions are essential trophic steroidogenic stimulation was rationalized. Through selection LH-responsive single-cell MA-10Slip clones, we uncovered cells remain...
In human patients and animal models of ulcerative colitis (UC), upregulation the mitochondrial translocator protein (TSPO) in colon is consistent with inflammation. Although molecular function for TSPO remains unclear, it has been investigated as a therapeutic target ameliorating UC pathology. this study, we examined susceptibility Tspo gene-deleted (Tspo-/- ) mice to insults provided by dextran sodium sulfate (DSS)-induced acute model. Our results show that clinical signs pathology were...
Conserved in female reproduction across all mammalian species is the estrous cycle and its regulation by hypothalamic-pituitary-gonadal (HPG) axis, a collective of intersected hormonal events that are crucial for ensuring uterine fertility. Nonetheless, knowledge direct mediators synchronously shape microenvironment successive yet distinct events, such as transit sperm support progressive stages preimplantation embryo development, remain principally deficient. Toward understanding timed...
Abstract Maternal-offspring coevolution has introduced the biological concept of “neonatal programming,” in which soluble proteins varying abundance bovine colostrum can have targeted activities calf gut. Still, identities and developmental programming mechanisms full profile on transiently expressed gut receptors/transporters, as well ultimate functional responses calf, remain to be completely elucidated. The objective this study was address gap knowledge using systems biology. First, we...
Abstract Escalating impacts of climate change present a credible threat to livestock-based food production. Increasing environmental challenges directly affect animal fitness, production performance, and reproductive efficiency with accumulative morbidities mortality. Albeit extant livestock breeds are adapted different extremes, the steep trajectory decline in genetic diversity poses serious sustenance farming. After pluripotent stem cells (PSCs) were derived for mice more than 30 years...