A5090441341- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA modifications and cancer
- Circular RNAs in diseases
- Cardiac Imaging and Diagnostics
- Cancer Immunotherapy and Biomarkers
- Ferroptosis and cancer prognosis
- Molecular Biology Techniques and Applications
- Extracellular vesicles in disease
- Chronic Obstructive Pulmonary Disease (COPD) Research
- RNA regulation and disease
- Autophagy in Disease and Therapy
- Cardiac Valve Diseases and Treatments
- Cardiac, Anesthesia and Surgical Outcomes
- Coronary Interventions and Diagnostics
- Genomics and Phylogenetic Studies
- Congenital Heart Disease Studies
- COVID-19 Clinical Research Studies
- Coastal and Marine Management
- Aortic Thrombus and Embolism
- Marine Ecology and Invasive Species
- Liver physiology and pathology
- Venous Thromboembolism Diagnosis and Management
- Healthcare and Venom Research
- Vasculitis and related conditions
Hummingbird Diagnostics (Germany)
2017-2024
Saarland University
2017-2021
Erzurum Regional Training and Research Hospital
2021
Stanford University
2019
Ludwigshafen University of Business and Society
2019
Heidelberg University
2019
Istanbul Technical University
2012
Yedikule Teaching Hospital
2011
Middle East Technical University
2001
Abstract Breast cancer is a heterogeneous disease with distinct molecular subtypes including the aggressive subtype triple-negative breast (TNBC). We compared blood-borne miRNA signatures of early-stage basal-like (cytokeratin-CK5-positive) TNBC patients to age-matched controls. The miRNAs were assessed prior and following platinum-based neoadjuvant chemotherapy (NCT). After an exploratory genome-wide study on 21 cases controls using microarrays, identified verified independently in two...
<h3>Importance</h3> The overall low survival rate of patients with lung cancer calls for improved detection tools to enable better treatment options and patient outcomes. Multivariable molecular signatures, such as blood-borne microRNA (miRNA) may have high rates sensitivity specificity but require additional studies large cohorts standardized measurements confirm the generalizability miRNA signatures. <h3>Objective</h3> To investigate use miRNAs potential circulating markers detecting in an...
Abstract Aging is a key risk factor for chronic diseases of the elderly. MicroRNAs regulate post-transcriptional gene silencing through base-pair binding on their target mRNAs. We identified nonlinear changes in age-related microRNAs by analyzing whole blood from 1334 healthy individuals. observed larger influence age as compared to sex and provide evidence shift 5’ mature form miRNAs aging. The addition 3059 diseased patients uncovered pan-disease disease-specific alterations aging...
MicroRNAs are regulators of gene expression. A wide-spread, yet not validated, assumption is that the targetome miRNAs non-randomly distributed across transcriptome and targets share functional pathways. We developed a computational experimental strategy termed high-throughput miRNA interaction reporter assay (HiTmIR) to facilitate validation target First, pathways predicted prioritized by means increase specificity positive predictive value. Second, novel webtool miRTaH facilitates guided...
Immunotherapies have recently gained traction as highly effective therapies in a subset of late-stage cancers. Unfortunately, only minority patients experience the remarkable benefits immunotherapies, whilst others fail to respond or even come harm through immune-related adverse events. For immunotherapies within PD-1/PD-L1 inhibitor class, patient stratification is currently performed using tumor (tissue-based) PD-L1 expression. However, an accurate predictor response ~30% cases. There...
To evaluate conjunctival cell microRNA (miRNAs) and mRNA expression in relation to observed phenotype of progressive limbal stem deficiency a cohort subjects with congenital aniridia known genetic status. Using impression cytology, bulbar cells were sampled from 20 age sex-matched healthy control subjects. RNA was extracted miRNA analyses performed using microarrays. Results related severity keratopathy cause aniridia. Of 2549 miRNAs, 21 differentially expressed relative controls (fold...
Abstract Chronic obstructive pulmonary disease (COPD) significantly increases the risk of developing cancer. Biomarker studies frequently follow a case-control set-up in which patients diagnosed with are compared to controls. Longitudinal cohort such as COPD-centered German COPD and SYstemic consequences-COmorbidities NETwork (COSYCONET) study provide patient biomaterial base for discovering predictive molecular markers. We asked whether microRNA (miRNA) profiles blood collected from prior...
Chronic obstructive pulmonary disease (COPD) is associated with an increased risk of death, reducing life expectancy on average between 5 and 7 years. The survival time after diagnosis, however, varies considerably as a result the heterogeneity COPD. Therefore, markers that predict individual COPD patients are great value. We analyzed baseline molecular profiles collected 54 months follow-up data cohort study “COPD SYstemic consequences-COmorbidities NETwork” (COSYCONET). Genome-wide...
Primary human hepatocytes (PHHs) undergo dedifferentiation upon the two-dimensional (2D) culture, which particularly hinders their utility in long-term vitro studies. Lipids, as a major class of biomolecules, play crucial roles cellular energy storage, structure, and signaling. Here, for first time, we mapped alterations lipid profile dedifferentiating PHHs studied possible role lipids loss phenotype PHHs. Simultaneously, differentially expressed miRNAs associated with changes fatty acids...
Abstract Motivation Although the amount of small non-coding RNA-sequencing data is continuously increasing, it still unclear to which extent RNAs are represented in human genome. Results In this study we analyzed 303 billion sequencing reads from nearly 25 000 datasets answer question. We determined that 0.8% genome reliably covered by 874 123 regions with an average length 31 nt. On basis these regions, found among known RNA classes, microRNAs were most prevalent. subsequent steps,...
MicroRNAs (miRNAs) are small RNAs regulating gene expression post-transcriptionally. While acquired changes of miRNA and mRNA profiles in cancer have been extensively studied, little is known about circulating miRNAs messenger (mRNA) monogenic constitutional anomalies affecting several organ systems, like Marfan syndrome (MFS). We performed integrated profiling blood samples patients order to investigate deregulated networks these which could serve as potential diagnostic prognostic tools...
Abstract miR-Blood is a high-quality, small RNA expression atlas for the major components of human peripheral blood (plasma, erythrocytes, thrombocytes, monocytes, neutrophils, eosinophils, basophils, natural killer cells, CD4+ T CD8+ and B cells). Based on purified from 52 individuals, dataset provides comprehensive repository 4971 RNAs eight non-coding classes.
Background Turner syndrome (TS) is a chromosomal disorder, in which female partially or entirely missing one of the two X chromosomes, with prevalence 1:2500 live births. The present study aims to identify circulating microRNA (miRNA) signature for TS patients and without congenital heart disease (CHD). Methods Microarray platform interrogating 2549 miRNAs were used detect miRNA abundance levels blood 33 14 age-matched healthy volunteer controls (HVs). differentially abundant between groups...
Different work flows have been proposed to use miRNAs as blood-borne biomarkers. In particular, the method used for collecting blood from patients can considerably influence diagnostic results.We explored whether dried spots (DBSs) facilitate stable miRNA measurements and compared its technical stability with biological variability. First, we tested of DBS samples by generating 1 person 18 whole-genome-wide profiles that were exposed different temperature humidity conditions. Second,...
The envisioned application of miRNAs as diagnostic or prognostic biomarkers calls for an in-depth understanding their distribution and variability in different physiological states. While effects with respect to ethnic origin, age, gender are known, the inter-individual across four seasons remained largely hidden. We sequentially profiled complete repertoire blood-borne 25 physiologically normal individuals spring, summer, fall, winter (altogether 95 samples) validated results on 292 (919...
The repertoire of small noncoding RNAs (sncRNAs), particularly miRNAs, in animals is considered to be evolutionarily conserved. Studies on sncRNAs are often largely based homology-based information, relying genomic sequence similarity and excluding actual expression data. To obtain information sncRNA (including snoRNAs, YRNAs tRNAs), we performed low-input-volume next-generation sequencing 500 pg RNA from 21 at two German zoological gardens. Notably, none the species under investigation were...
ABSTRACT Small RNAs hold crucial biological information and have immense diagnostic therapeutic value. While many established annotation tools focus on microRNAs, there are myriads of other small that currently underutilized. These can be difficult to annotate, as ground truth is limited well-established mapping mismatch rules lacking. TransfoRNA a machine learning framework based Transformers explores an alternative strategy. It uses common generate seed high-confidence training labels,...
Patients with advanced, non-oncogene-driven NSCLC high programmed death-ligand 1 (PD-L1) expression are eligible for treatment immunotherapy. There is, however, an urgent medical need biomarkers identifying cases that require additional combination chemotherapy. We previously uncovered a myeloid-based 5-microRNA (5-miRNA) signature identified responders to immunotherapy in PD-L1 unstratified patients; its potential utility guidance patients tumors remained unclear.We trained (n = 68) and...
Abstract Arteriovenous (AV) fistulas for hemodialysis can lead to cardiac volume loading and increased serum brain natriuretic peptide (BNP) levels. Whether short-term AV loop placement in patients undergoing microsurgery has an impact on biomarkers circulating microRNAs (miRNAs), potentially indicating hemodynamic risk, remains elusive. Fifteen underwent with delayed free flap anastomosis microsurgical reconstructions of lower extremity soft-tissue defects. N-terminal pro-BNP (NT-proBNP),...
Biochemical markers are needed to show lung involvement in COVID-19 disease. Galectin-3 is known play a key role the inflammation and fibrosis process. We aimed evaluate predictive of galectin-3 levels for pneumonia patients with COVID-19.Total 176 COVID-19, confirmed reverse transcriptase polymerase chain reaction, admitted Erzurum Regional Training Research Hospital was analyzed. The study designed as cross sectional. baseline data laboratory examinations, including were collected at time...
Aim: Chest CT plays an important role in the treatment and diagnosis of COVID-19.It is to remember that patients with RT-PCR test positive for COVID-19 infection may have normal chest CT.Material Methods: This study included who underwent tests, as well suspected confirmed cases according algorithm Institute Science Ministry Health Turkey were this study.The divided into two groups negative results.These subgroups: findings compatible without them.When was taken gold standard, specificity,...