A5062821059- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Gut microbiota and health
- SARS-CoV-2 detection and testing
- Single-cell and spatial transcriptomics
- RNA regulation and disease
- Mycobacterium research and diagnosis
- Bacteriophages and microbial interactions
- vaccines and immunoinformatics approaches
- Sepsis Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Mosquito-borne diseases and control
- Extracellular vesicles in disease
- HIV Research and Treatment
- COVID-19 diagnosis using AI
- RNA modifications and cancer
- Immune responses and vaccinations
- Genomics and Phylogenetic Studies
- Machine Learning in Bioinformatics
- Immune Cell Function and Interaction
- Bacillus and Francisella bacterial research
- Diet and metabolism studies
- RNA Research and Splicing
- Viral Infections and Immunology Research
Institute of Genomics and Integrative Biology
2020-2025
Academy of Scientific and Innovative Research
2020-2025
College of Medicine & Sagore Dutta Hospital
2016-2023
Intel (India)
2022
Council of Scientific and Industrial Research
2021
Medical College and Hospital, Kolkata
2017
The B.1.617.2 (Delta) variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first identified in the state Maharashtra late 2020 and spread throughout India, outcompeting pre-existing lineages including B.1.617.1 (Kappa) B.1.1.7 (Alpha)
Abstract The SARS-CoV-2 B.1.617.2 (Delta) variant was first identified in the state of Maharashtra late 2020 and spread throughout India, outcompeting pre-existing lineages including B.1.617.1 (Kappa) B.1.1.7 (Alpha). In vitro , is 6-fold less sensitive to serum neutralising antibodies from recovered individuals, 8-fold vaccine-elicited as compared wild type Wuhan-1 bearing D614G. Serum titres against were lower ChAdOx-1 versus BNT162b2 vaccinees. spike pseudotyped viruses exhibited...
<title>Abstract</title> The SARS-CoV-2 B.1.617.2 (Delta) variant was first identified in the state of Maharashtra late 2020 and has spread throughout India, displacing B.1.1.7 (Alpha) other pre-existing lineages. Mathematical modelling indicates that growth advantage is most likely explained by a combination increased transmissibility immune evasion. Indeed vitro, delta less sensitive to neutralising antibodies sera from recovered individuals, with higher replication efficiency as compared...
The variability of clinical course and prognosis COVID-19 highlights the necessity patient sub-group risk stratification based on data. In this study, data from a cohort Indian hospitalized patients is used to develop mortality prediction models. We analyzed set 70 parameters including physiological hematological for developing machine learning models identify biomarkers. also compared Wuhan cohort, role steroids. A bootstrap averaged ensemble Bayesian networks was learned construct an...
The modulators of severe COVID-19 have emerged as the most intriguing features SARS-CoV-2 pathogenesis. This is especially true we are encountering variants concern (VOC) with increased transmissibility and vaccination breakthroughs. Microbial co-infections being investigated one crucial factors for exacerbation disease severity complications COVID-19. A key question remains whether early transcriptionally active microbial signature/s in patients can provide a window future susceptibility...
The Omicron variant of SARS-CoV-2 is capable infecting unvaccinated, vaccinated and previously-infected individuals due to its ability evade neutralization by antibodies. With multiple sub-lineages emerging in the last 12 months, there inadequate information on quantitative antibody response generated upon natural infection with whether these antibodies offer cross-protection against other variant. In this study, we characterized growth kinetics Kappa, Delta variants Calu-3 cells. Relatively...
Co-infection with ancillary pathogens is a significant modulator of morbidity and mortality in infectious diseases. There have been limited reports co-infections accompanying SARS-CoV-2 infections, albeit lacking India specific study. The present study has made an effort toward elucidating the prevalence, diversity characterization co-infecting respiratory nasopharyngeal tract positive patients. Two complementary metagenomics based sequencing approaches, Respiratory Virus Oligo Panel (RVOP)...
Dengue fever, a neglected tropical arboviral disease, has emerged as global health concern in the past decade. Necessitating nuanced comprehension of intricate dynamics host–virus interactions influencing disease severity, we analysed transcriptomic patterns using bulk RNA-seq from 112 age- and gender-matched NS1 antigen–confirmed hospital-admitted dengue patients with varying severity. Severe cases exhibited reduced platelet count, increased lymphocytosis, neutropenia, indicating...
Introduction Despite numerous efforts to describe COVID-19's immunological landscape, there is still a gap in our understanding of the virus's infections after-effects, especially recovered patients. This would be important understand as we now have huge number global populations infected by SARS-CoV-2 well variables inclusive VOCs, reinfections, and vaccination breakthroughs. Furthermore, single-cell transcriptome alone often insufficient complex human host immune landscape underlying...
The emergence of multiple variants concerns (VOCs) with higher number Spike mutations have led to enhanced immune escape by the SARS-CoV-2. With increasing vaccination breakthrough (VBT) infections, it is important understand possible reason/s infections.
This study elucidated the clinical, humoral immune response and genomic analysis of vaccine breakthrough (VBT) infections after ChAdOx1 nCoV-19/Covishield in healthcare workers (HCWs). Amongst 1858 HCWs, 1639 had received either two doses (1346) or a single dose (293) nCoV-19 vaccine. SARS-CoV-2 IgG antibodies neutralizing were measured vaccinated group development infection was monitored.Forty-six RT-PCR positive samples from 203 subjected to whole genome sequencing (WGS). Of (10.92%)...
Emergence of SARS-CoV-2 VOCs at different time points through COVID-19 pandemic raised concern for increased transmissibility, infectivity and vaccination breakthroughs.1567 international travellers plus community transmission cases were analysed mutational profile VOCS, that led to notable waves in India, namely Alpha, Delta, Omicron. Spike mutations Linkage Disequilibrium investigated potential impact on structural functional changes Spike-ACE2.ORF1ab spike harboured diverse signatures...
Intracellular microorganisms, like viruses, bacteria, and fungi, pose challenges in detection due to their non-culturable forms. Transcriptomic analysis at cellular level enables exploration of distributions the impact these microorganisms on host cells, a domain that remains underexplored because methodological limitations. Single-cell technology shows promise addressing this by capturing polyadenine-tailed transcripts, recent studies confirmed polyadenylation microbial transcriptomes. We...
Introduction Single-cell multi-omics studies, such as multidimensional transcriptomics (whole transcriptomic analysis, WTA), and surface marker analysis (antibody sequencing, AbSeq), have turned out to be valuable techniques that offer inaccessible possibilities for single-cell profiling of mRNA, lncRNA, proteins. Methods We used this technique understand the dynamics mRNA protein-level differences in healthy, COVID-19-infected recovered individuals using peripheral blood mononuclear cells...
Disease caused by SARS-CoV-2 coronavirus (COVID-19) led to significant morbidity and mortality worldwide. A systemic hyper-inflammation characterizes severe COVID-19 disease, often associated with acute respiratory distress syndrome (ARDS). Blood biomarkers capable of risk stratification are great importance in effective triage critical care patients. Flow cytometry next-generation sequencing were done on peripheral blood cells urokinase-type plasminogen activator receptor (suPAR), cytokines...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) manifests a broad spectrum of clinical presentations, varying in severity from asymptomatic to mortality. As the viral infection spread, it evolved and developed into many variants concern. Understanding impact mutations SARS-CoV-2 genome on phenotype associated co-morbidities is important for treatment preventionas pandemic progresses. Based mild, moderate, severe phenotypes, we analyzed possible association between both,...
Abstract A plethora of studies have demonstrated the roles lncRNAs in modulating disease severity and outcomes during infection. However, spatio-temporal expression these is poorly understood. In this study, we used single-cell RNA-seq to understand dynamics across healthy, SARS-CoV-2-infected, recovered individuals their functional role recovery. We identified 203 differentially expressed lncRNAs, including cell type-specific ones like MALAT1, NEAT1, ZFAS1, SNHG7, SNHG8, SNHG25 immune...
Abstract Background India saw a massive surge and emergence of SARS CoV2 variants. We elucidated clinical humoral immune response genomic analysis vaccine breakthrough (VBT) infections after ChAdOx1 nCoV-19 in healthcare workers (HCWs). Methods The study was conducted on 1858 HCWs receiving two doses vaccine. Serial blood samples were collected to measure IgG neutralizing antibodies. 46 RT-PCR positive from VBT subjected whole genome sequencing (WGS). Results Infection confirmed 219 (11.79%)...
ABSTRACT Like single-stranded RNA viruses, SARS-CoV-2 hijacks the host transcriptional machinery for its own replication. Numerous traditional differential gene expression-based investigations have examined diverse clinical symptoms caused by infection. The virus, on other hand, also affects splicing machinery, causing dysregulation, which can lead to outcomes. Hence, in this study, we performed transcriptome sequencing of 125 hospital-admitted COVID-19 patients understand transcriptomic...