A5029836475- Neuroblastoma Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Glioma Diagnosis and Treatment
- Ferroptosis and cancer prognosis
- MicroRNA in disease regulation
- Immune cells in cancer
- Virus-based gene therapy research
- Adrenal and Paraganglionic Tumors
- Acute Myeloid Leukemia Research
- Cancer, Lipids, and Metabolism
- Renal Transplantation Outcomes and Treatments
- Cancer Genomics and Diagnostics
- Lung Cancer Research Studies
- Chromatin Remodeling and Cancer
- Complement system in diseases
- Cancer Immunotherapy and Biomarkers
- Cell Adhesion Molecules Research
- Autoimmune and Inflammatory Disorders Research
- Genomic variations and chromosomal abnormalities
- Hedgehog Signaling Pathway Studies
- Hemoglobinopathies and Related Disorders
- Cancer Research and Treatments
- Neurofibromatosis and Schwannoma Cases
- Cellular Mechanics and Interactions
- Nanoplatforms for cancer theranostics
Children's Hospital of Los Angeles
2015-2025
University of Southern California
2005-2024
Children's Center
2015-2020
Nationwide Children's Hospital
2015
The University of Texas MD Anderson Cancer Center
2015
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori
2015
Istituti di Ricovero e Cura a Carattere Scientifico
2015
Cincinnati Children's Hospital Medical Center
2005-2013
Children's Hospital of Philadelphia
2005
Children's Oncology Group
2005
How exosomic microRNAs (miRNAs) contribute to the development of drug resistance in context tumor microenvironment has not been previously described neuroblastoma (NBL). Coculture experiments were performed assess transfer miR-21 from NBL cells human monocytes and miR-155 cells. Luciferase reporter assays targeting TERF1 Tumor growth was measured xenografts treated with Cisplatin peritumoral (n = 6 mice per group) CD163, miR-155, levels assessed 20 primary tissues by Human Exon Arrays...
<h3>OBJECTIVE</h3> To quantify the percentage of apoptotic peripheral blood neutrophils in systemic lupus erythematosus (SLE) and to determine relations with disease activity neutropenia. <h3>METHODS</h3> Neutrophil apoptosis SLE patients (n =50) was assessed by flow cytometry using annexin V binding fluorescent labelled anti-fas. Rheumatoid arthritis (RA, n =20) inflammatory bowel (IBD, were studied as controls. <h3>RESULTS</h3> The neutrophils, determined binding, increased (median =...
Medulloblastoma in children can be categorized into at least four molecular subgroups, offering the potential for targeted therapeutic approaches to reduce treatment-related morbidities. Little is known about role of tumor microenvironment medulloblastoma or its contribution these subgroups. Tumor has been shown an important source targets both adult and pediatric neoplasms. In this study, we investigated hypothesis that expression genes related tumor-associated macrophages (TAM) correlates...
Medulloblastomas in children can be categorized into 4 molecular subgroups with differing clinical characteristics, such that subgroup determination aids prognostication and risk-adaptive treatment strategies. Magnetic resonance spectroscopy (MRS) is a widely available, noninvasive tool used to determine the metabolic characteristics of tumors provide diagnostic information without need for tumor tissue. In this study, we investigated hypothesis metabolite concentrations measured by MRS...
// Michael D. Hadjidaniel 1, * , Sakunthala Muthugounder Long T. Hung A. Sheard 1 Soheila Shirinbak Randall Y. Chan 3 Rie Nakata Lucia Borriello Jemily Malvar Rebekah J. Kennedy Hiroshi Iwakura 2 Takashi Akamizu Richard Sposto Hiroyuki Shimada Yves DeClerck and Shahab Asgharzadeh Children's Hospital Los Angeles, Center for Cancer Blood Diseases, Division of Hematology, Oncology & Marrow Transplantation, The Saban Research Institute, CA, USA First Department Medicine, Wakayama Medical...
Tumor-associated macrophages (TAM) and cancer-associated fibroblasts (CAF) their precursor mesenchymal stromal cells (MSC) are often detected together in tumors, but how they cooperate is not well understood. Here, we show that TAM CAF the most abundant nonmalignant present untreated human neuroblastoma (NB) tumors also poorly infiltrated with T natural killer (NK) cells. We then MSC CAF-MSC harvested from NB protected monocytes (MN) spontaneous apoptosis an interleukin (IL)-6 dependent...
Abstract Background Intrinsic and extrinsic factors in the tumour microenvironment (TME) contribute to therapeutic resistance. Here we demonstrate that transforming growth factor (TGF)-β1 produced TME increased drug resistance of neuroblastoma (NB) cells. Methods Human NB cell lines were tested vitro for their sensitivity Doxorubicin (DOX) Etoposide (ETOP) presence tumour-associated macrophages (TAM) mesenchymal stromal cells/cancer-associated fibroblasts (MSC/CAF). These experiments...
Purpose: We determined whether quantifying neuroblastoma-associated mRNAs (NB-mRNAs) in bone marrow and blood improves assessment of disease prediction progression patients with relapsed/refractory neuroblastoma.Experimental Design: mRNA for CHGA, DCX, DDC, PHOX2B, TH was quantified from 101 concurrently clinical evaluations. Correlation between NB-mRNA (delta cycle threshold, ΔCt, the geometric mean genes TaqMan Low Density Array NB5 assay) morphologically defined tumor cell percentage...
Immune checkpoint therapy has resulted in minimal clinical response many pediatric cancers. We sought to understand the influence of immune inhibition using anti-PD-1 and anti-CTLA-4 antibodies individually, combination, after chemotherapy on responses established murine neuroblastoma models. also role tumor microenvironment (TME) PD-L1 expression their alteration post-chemotherapy our models human tissues. was enriched tumor-associated macrophages up-regulated chemotherapy. In a disease...
Patients ≥18 months of age with International Neuroblastoma Staging System (INSS) stage 3 unfavorable histology (UH), MYCN-nonamplified (MYCN-NA) tumors have favorable survival rates compared other high-risk neuroblastoma populations. The impact select clinical and biological factors on overall (OS) event-free (EFS) were evaluated.Patients enrolled Children's Oncology Group (COG) A3973 (n = 34), ANBL0532 27), and/or biology protocol ANBL00B1 72) analyzed. Tumors available DNA 65) RNA 42)...
Lupus specific autoantigens are exposed on apoptotic cells. The increased number of lymphocytes reported in systemic lupus erythematosus (SLE) may be attributable to abnormalities lymphocyte Fas expression or serum soluble Fas. In the present study we analysed count circulating SLE patients (n ‘ 50), by flow cytometry using Annexin V, compared rheumatoid arthritis (RA, n 20), inflammatory bowel disease (IBD, 20) and normal controls 20). Lymphocyte were measured related numbers lymphocytes....
Abstract Neuroblastoma (NB), the most common extracranial solid tumor in children, exhibits intra-tumoral heterogeneity with two interconvertible identities: adrenergic (ADRN) and mesenchymal (MES). MES cells display phenotypes associated metastasis resistance to therapies compared ADRN. Thus, reprogramming of ADRN may contribute poor NB outcomes, process requires further elucidation improve clinical outcomes. The extracellular matrix (ECM), a critical component microenvironment (TME),...
Medulloblastoma is a malignant embryonal tumor of the cerebellum that occurs predominantly in children. To find germline genetic variants associated with medulloblastoma risk, we conducted genome-wide association study (GWAS) including 244 cases and 247 control subjects from Sweden Denmark.
Abstract Neuroblastoma (NB), the most common extracranial solid tumor in children, exhibits significant intra-tumoral heterogeneity with two interconvertible identities: adrenergic (ADRN) and mesenchymal (MES). MES cells exhibit phenotypes associated metastasis are enriched relapse NB compared to ADRN. Thus, reprogramming from ADRN may determine inferior outcomes, which needs better elucidation. Extracellular matrix (ECM) is an essential microenvironment (TME) component that provides...
Abstract Background Minimal disease quantification may predict event‐free survival (EFS) and overall (OS). Methods We evaluated mRNA expression of five neuroblastoma‐associated genes (NB5 assay) in bone marrows (BM) patients with newly diagnosed high‐risk neuroblastoma who received consistent immunotherapy. CHGA, DCX, DDC, PHOX2B, TH BM 479 enrolled on the immunotherapy arm Children's Oncology Group trials ANBL0032 ANBL0931 was using real‐time polymerase chain reaction (PCR)‐based TaqMan...
Abstract The concept of tumor-promoting inflammation is a recognized enabling characteristic cancer. Tumor associated macrophages (TAMs), one the main contributors to tumor microenvironment, have been identified in many adult malignancies and MYCN non-amplified high risk neuroblastomas. TAMs shown promote cancer via multiple mechanisms including cell growth survival, invasion, metastasis, angiogenesis, inflammation, immune regulation. Recent studies gliomas demonstrated increased expression...
Current diagnostic criteria for TMA proposed by the Blood and Marrow Transplant Clinical Trials Network International Working Group have limitations because renal hematologic findings are not specific after HSCT, as noted retrospectively Cho et al (Transplantation 2010). To prospectively assess utility of current we enrolled 100 pediatric HSCT patients at a single institution evaluated clinical laboratory markers during first days post-HSCT (Table 1). Patients with biopsy diagnosis or signs...
<p>Change in BM and Blood NB5 �Ct for patients with progressive disease vs. non-progressive disease. Change was assessed between pairs of time points where both clinical assessments were performed. Observations are classified by whether there progression or not the points.</p>
<p>Comparison of 1 year EFS in two groups defined by a range ∆CT cut points (left column) and corresponding ROC analysis the ability values above cutpoint to predict (right column), for assays based on different combinations genes. NB5 assay (A, B), Phox2B/TH/DDC (C, D), Phox2B/TH (E, F), Phox2B/TH/Dcx (G, H), Th/Dcx (I, J). These analyses show that is at least as or more predictive then other gene combinations.</p>
<p>Supplementary Figure Legends for A1-A3</p>
<p>Correlation of morphologically-defined NB cells in BM and MIBG Curie score. Analysis biopsies scans were performed concurrently on 173 patients.</p>