A5003206659- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- CAR-T cell therapy research
- Chemotherapy-induced cardiotoxicity and mitigation
- RNA and protein synthesis mechanisms
- Radiopharmaceutical Chemistry and Applications
- Hemoglobinopathies and Related Disorders
- Iron Metabolism and Disorders
- X-ray Diffraction in Crystallography
- Neonatal Health and Biochemistry
- Metal complexes synthesis and properties
- Immune Cell Function and Interaction
- Erythrocyte Function and Pathophysiology
- Crystallization and Solubility Studies
- Photosynthetic Processes and Mechanisms
- Cancer, Hypoxia, and Metabolism
- Lung Cancer Treatments and Mutations
- Pancreatic function and diabetes
- Cancer therapeutics and mechanisms
- Coenzyme Q10 studies and effects
- Porphyrin Metabolism and Disorders
- Hemoglobin structure and function
- Ferrocene Chemistry and Applications
Aristotle University of Thessaloniki
2013-2024
Bicêtre Hospital
2018
We screened 41 patients with undiagnosed encephalomyopathies and cytochrome c oxidase (COX) deficiency for mutations in two COX assembly genes, SURF-1 SCO2; 6 had 3 SCO2. All of the were small-scale rearrangements (deletions/insertions); homozygotes other compound heterozygotes. SCO2 heterozygotes nonsense or missense mutations. Leigh syndrome, whereas a combination encephalopathy hypertrophic cardiomyopathy, neuropathology did not show typical features syndrome. In mutations, onset was...
Inheritance of animal mtDNA is almost exclusively maternal, most likely because sperm-derived mitochondria are actively eliminated from the ovum, either at or soon after fertilization. How such elimination occurs currently unknown. We asked whether similar behavior could be detected in somatic cells, by following fate and mtDNAs entry human sperm into transformed cells containing but lacking endogenous (rho0 cells). found that a high proportion (10%-20%) contained functioning entry. However,...
The potential clinical applications of the powerful in vitro-transcribed (IVT)-mRNAs, to restore defective protein functions, strongly depend on their successful intracellular delivery and transient translation through development safe efficient platforms. In this study, an innovative (international patent-pending) methodology was developed, combining IVT-mRNAs with transduction domain (PTD) technology, as platform. Based PTD which enables various cargoes intracellularly, conjugation a...
Introduction: Nowadays, nanotechnology and genomics contribute knowledge innovative practices, enabling pharmacological interventions as well theraputic decisions to be applicable on a personal basis, i.e., establishing precision (personalized) medicine approaches into the clinical setting. However, dynamics evolution capacity of organisms over time necessitate advancement powerful interdisciplinary tools, population-based methodologies, application translational methodologies with...
The erythroid related disorders (ERDs) represent a large group of hematological diseases, which in most cases are attributed either to the deficiency or malfunction biosynthetic enzymes oxygen transport proteins. Current treatments for these include histo-compatible erythrocyte transfusions allogeneic hematopoietic stem cell (HSC) transplantation. Gene therapy delivered via suitable viral vectors genetically modified HSCs have been under way. Protein Transduction Domain (PTD) technology has...
The rapid progress achieved in the development of many biopharmaceuticals had a tremendous impact on therapy metabolic/genetic disorders. This type fruitful approach, called protein replacement (PRT), aimed to either replace deficient or malfunctional human tissues that act plasma membrane via specific cell surface receptor. However, there are also disorders attributed proteins acting intracellularly. recent developments Protein Transduction Domain (PTD) technology offer new opportunities by...
Tumor malignant cells are characterized by dysregulation of mitochondrial bioenergetics due to the 'Warburg effect'. In present study, this metabolic imbalance was explored as a potential target for novel cancer chemotherapy. Imatinib (IM) downregulates expression levels
Protein replacement therapy (PRT) has been applied to treat severe monogenetic/metabolic disorders characterized by a protein deficiency. In where an intracellular is missing, PRT not easily feasible due the inability of proteins cross cell membrane. Instead, gene applied, although still with limited success. β-Thalassemias are congenital hemoglobinopathies, deficiency or reduced production adult β-globin chain. The resulting imbalance α-/β-globin chains hemoglobin (α2β2) leads precipitation...
Chimeric antigen receptor (CAR) immunotherapy includes the genetic modification of immune cells to carry such a and, thus, recognize cancer cell surface antigens. Viral transfection is currently preferred method, but it carries risk off-target mutagenicity. Other platforms have thus been proposed, in vitro transcribed (IVT)-mRNAs. In this study, we exploited our innovative, patented delivery platform produce protein transduction domain (PTD)-IVT-mRNAs for expression CAR on NK-92 cells....