A5080073991- COVID-19 diagnosis using AI
- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- BRCA gene mutations in cancer
- RNA modifications and cancer
- Insect Resistance and Genetics
- Insect and Arachnid Ecology and Behavior
- Effects of Radiation Exposure
- Insect-Plant Interactions and Control
- Plant Reproductive Biology
- Neurobiology and Insect Physiology Research
- Epigenetics and DNA Methylation
- Tryptophan and brain disorders
- Cancer-related molecular mechanisms research
- Plant Virus Research Studies
- Insect symbiosis and bacterial influences
- Radiation Therapy and Dosimetry
- Plant and animal studies
- Genetically Modified Organisms Research
- Muscle metabolism and nutrition
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- Exercise and Physiological Responses
- Insect Utilization and Effects
- Genetic Associations and Epidemiology
University of Nebraska Medical Center
2017-2024
Rajiv Gandhi Centre for Biotechnology
2012-2024
University of Nebraska–Lincoln
2014-2022
Nebraska Medical Center
2018
The wing polyphenism of pea aphids is a compelling laboratory model with which to study the molecular mechanisms underlying phenotypic plasticity. In this polyphenism, environmental stressors such as high aphid density cause asexual, viviparous adult female alter developmental fate their embryos from wingless winged morphs. This transgenerational, in that mother experiences signals, but it her offspring are affected. Previous research suggested steroid hormone ecdysone may play role...
DNA methylation has been implicated in the etiopathology of various complex disorders. methyltransferases are involved maintaining and establishing new patterns. The aim present study was to investigate inherent genetic variations within methyltransferase genes predisposing susceptibility schizophrenia. We screened for polymorphisms methyltransferases, DNMT1, DNMT3A, DNMT3B DNMT3L 330 schizophrenia patients 302 healthy controls association with Schizophrenia south Indian population. These...
Abstract Phenotypic plasticity is a key life history strategy used by many plants and animals living in heterogeneous environments. A multitude of studies have investigated the costs limits plasticity, as well conditions under which it evolves. Much less understood are molecular genetic mechanisms that enable an organism to sense its environment respond plastic manner. The pea aphid wing polyphenism compelling laboratory model study these mechanisms. In this polyphenism, environmental...
Nrf2 is a master regulator of endogenous cellular defences, governing the expression more than 200 cytoprotective proteins, including panel antioxidant enzymes. plays an important role in redox haemostasis skeletal muscle response to increased generation reactive oxygen species during contraction. Employing muscle-specific transgenic mouse models with unbiased-omic approaches, we uncovered new target downstream pathways and molecular networks following or Keap1 deletion. Based on findings,...
In schizophrenia, genetic background may provide a substrate for intrinsic maldevelopment of the brain through environmental influences, by recruiting neurotrophic factors and cytokines, to trigger changes that lead impaired neuronal functions. Cytokines being key regulators immune/inflammatory reactions are also known influence dopaminergic, noradrenergic, serotonergic neurotransmission. Therefore, functional polymorphisms in cytokine genes result imbalances pro- anti-inflammatory...
Gene fusions that contribute to oncogenicity can be explored for identifying cancer biomarkers and potential drug targets. To investigate the nature distribution of fusion transcripts in cancer, we examined transcriptome data about 9,000 primary tumors from 33 different cancers TCGA (The Cancer Genome Atlas) along with cell line CCLE (Cancer Cell Line Encyclopedia) using ChimeRScope, a novel detection algorithm. We identified several sense (canonical, 39%) or antisense (non-canonical, 61%)...
Abstract Objective India reported its first coronavirus disease 2019 (COVID-19) case in the state of Kerala and an outbreak initiated subsequently. The Department Health Services, Government Kerala, initially released daily updates through textual bulletins for public awareness to control spread disease. However, these unstructured data limit upstream applications, such as visualization, analysis, thus demanding refinement generate open reusable datasets. Materials Methods Through a citizen...
The focus of radiation biodosimetry has changed recently, and a paradigm shift for using molecular technologies omic platforms in addition to cytogenetic techniques been observed. In our study, we have used nonhuman primate model investigate the impact supralethal dose 12 Gy on alterations lung transcriptome. We 6 healthy 32 irradiated animal samples delineate radiation-induced changes. also medical countermeasure, γ-tocotrienol (GT3), observe any demonstrate significant changes...
Adaptations by arthropod pests to host plant defenses of crops determine their impacts on agricultural production. The larval range western corn rootworm, Diabrotica virgifera (Coleoptera: Chrysomelidae), is restricted maize and a few grasses. Resistance D. v. crop rotation practices multiple insecticides contributes its status as the most damaging pest cultivated in North America Europe. extent which adaptations this specialization remains unknown.A 2.42 Gb draft genome, Dvir_v2.0, was...
Exercise training (ExT) improves skeletal muscle health via multiple adaptative pathways. Nrf2 is a principal antioxidant transcription factor responsible for maintaining intracellular redox homeostasis. In this study, we hypothesized that essential responses to ExT and thus beneficial muscle. Experiments were carried out on male wild type (WT) iMS-Nrf2flox/flox inducible muscle-specific (KO) mice, which randomly assigned serve as sedentary controls (Sed) or underwent 3 weeks of treadmill...
Wing polymorphism is a powerful model for examining many aspects of adaptation. The wing dimorphic cricket species, Gryllus firmus, consists long-winged morph with functional flight muscles that capable flight, and two flightless morphs. One (obligately) emerges as an adult vestigial wings muscles. other (plastic) fully-developed but later in adulthood histolyzes its Importantly both morphs have substantially increased reproductive output relative to the flight-capable morph. Much known...
Despite a number of recent reports insect resistance to transgenic crops expressing insecticidal toxins from Bacillus thuringiensis (Bt), little is known about the mechanism these toxins. The purpose this study identify genes associated with Cry1F toxin in European corn borer (Ostrinia nubilalis Hübner). For this, we compared global transcriptomic response laboratory selected resistant and susceptible O. strain toxin. We further identified constitutive transcriptional differences between two...
Wing dimorphisms have long served as models for examining the ecological and evolutionary tradeoffs associated with alternative phenotypes. Here, we investigated genetic cause of pea aphid (Acyrthosiphon pisum) male wing dimorphism, wherein males exhibit one two morphologies that differ in correlated traits include presence or absence wings. We mapped this trait difference to a single genomic region and, using third generation, long-read sequencing, identified 120 kb insertion wingless...
The RNA-Seq technology has revolutionized transcriptome characterization not only by accurately quantifying gene expression, but also the identification of novel transcripts like chimeric fusion transcripts. 'fusion' or 'chimeric' have improved diagnosis and prognosis several tumors, led to development therapeutic regimen. transcript detection is currently accomplished software packages, primarily relying on sequence alignment algorithms. sequencing reads from loci in cancer genomes can be...
Smoking remains a significant health and economic concern in the United States. Furthermore, emerging pattern of nicotine intake between sexes further adds layer complexity. Nicotine is potent psychostimulant with high addiction liability that can significantly alter brain function. However, neurobiological mechanisms underlying nicotine's impact on function behavior remain unclear. Elucidation these clinical importance may lead to improved therapeutics for smoking cessation. To fill this...
Abstract The risk of exposure the general public or military personnel to high levels ionizing radiation from nuclear weapons radiological accidents is a dire national security matter. development advanced molecular biodosimetry methods, those that measure biological response, such as transcriptomics, screen large populations radiation-exposed victims key improving survival outcomes during mass casualty scenarios. In this study, nonhuman primates were exposed either 12.0 Gy cobalt-60 gamma...
Schizophrenia is a complex condition with entwined genetic and epigenetic risk factors, posing challenge to disentangle the intermixed pathological therapeutic signatures. To resolve this, we performed 850K methylome-wide 700K genome-wide studies on same set of schizophrenia patients by stratifying them into responders, non-responders, drug-naïve patients. The key genes that signified response were followed up using real-time gene expression understand effect antipsychotics at transcription...
Extracellular vesicles (EVs) are nanosized that have been garnering a lot of attention for their valuable role as potential diagnostic markers and therapeutic vehicles plethora pathologies. While EV from biofluids such plasma, serum, urine, cerebrospinal fluid, in vitro cell culture-based platforms extensively studied, significant knowledge gap remains is the characterization specific organ-derived EVs (ODE). Here, we present standardized protocol isolation purified isolated brain, heart,...
The intensive use of anesthetic and sedative agents in the neonatal care unit (NICU) has raised controversial concerns about potential neurodevelopmental risks. This study focused on midazolam (MDZ), a common benzodiazepine regularly used as neonates NICU. Mounting evidence suggests single exposure to MDZ during period leads learning disturbances. However, knowledge gap that remains is how long-term very early stages life impacts synaptic alterations. Using preclinical rodent model system,...
The current opioid crisis, which has ravaged all segments of society, continues to pose a rising public health concern. Importantly, dependency on prescription opioids such as oxycodone (oxy) during and after pregnancy can significantly impact the overall brain development exposed offspring, especially at synapse. A significant knowledge gap that remains is identifying distinct synaptic signatures associated with these offspring. Accordingly, goal this study was identify vesicle (SV)...
Cardiorenal syndrome type 2 (CRS2) is defined as a chronic cardiovascular disease, usually heart failure (CHF), resulting in kidney disease. We hypothesized that the cardiac spinal afferent reflex (CSAR) plays critical role development of CRS2. Our data suggest ablation by resiniferatoxin not only improves function but also benefits kidneys and increases long-term survival myocardial infarction model CHF. found renal denervation has similar reno-protective effect CHF rats. believe this novel...