A5037365101- Liver Disease Diagnosis and Treatment
- Alcohol Consumption and Health Effects
- Retinal Diseases and Treatments
- Diet, Metabolism, and Disease
- Ocular Diseases and Behçet’s Syndrome
- Viral gastroenteritis research and epidemiology
- Neuroinflammation and Neurodegeneration Mechanisms
- Reproductive tract infections research
- Salmonella and Campylobacter epidemiology
- Retinal and Optic Conditions
- Liver Disease and Transplantation
- Chemokine receptors and signaling
- Urinary Tract Infections Management
- Genetic Associations and Epidemiology
- Immunotherapy and Immune Responses
- Sphingolipid Metabolism and Signaling
- Bacteriophages and microbial interactions
- Genital Health and Disease
- interferon and immune responses
- Gut microbiota and health
- Cervical Cancer and HPV Research
- Clostridium difficile and Clostridium perfringens research
- Gastrointestinal motility and disorders
- Advanced Proteomics Techniques and Applications
- Adipose Tissue and Metabolism
Novo Nordisk Foundation
2020-2025
University of Copenhagen
2013-2025
Statens Serum Institut
2018-2024
Novo Nordisk Foundation Center for Basic Metabolic Research
2022
Heidelberg University
2022
University Hospital Heidelberg
2022
University of Oxford
2017-2020
National Institute for Health Research
2020
Oxford BioMedica (United Kingdom)
2017-2019
Psykiatrien i Region Syddanmark
2013
Salmonella enterica serovar Typhi (S Typhi) is responsible for an estimated 20 million infections and 200 000 deaths each year in resource poor regions of the world. Capsular Vi-polysaccharide-protein conjugate vaccines (Vi-conjugate vaccines) are immunogenic can be used from infancy but there no efficacy data leading candidate vaccine being considered widespread use. To address this knowledge gap, we assessed a Vi-tetanus toxoid using established human infection model S Typhi.
Abstract Our current understanding of the determinants plasma proteome variation during pediatric development remains incomplete. Here, we show that genetic variants, age, sex and body mass index significantly influence this variation. Using a streamlined highly quantitative spectrometry-based proteomics workflow, analyzed from 2,147 children adolescents, identifying 1,216 proteins after quality control. Notably, levels 70% these were associated with at least one aforementioned factors,...
The microbiota in individual habitats differ both relative composition and absolute abundance. While sequencing approaches determine the abundances of taxa genes, they do not provide information on their abundances. Here, we developed a machine-learning approach to predict fecal microbial loads (microbial cells per gram) solely from abundance data. Applying our prediction model large-scale metagenomic dataset (n = 34,539), demonstrated that load is major determinant gut microbiome variation...
Pediatric obesity is a progressive, chronic disease that can lead to serious cardiometabolic complications. Here we investigated the peripheral lipidome in 958 children and adolescents with overweight or 373 normal weight, cross-sectional study. We also implemented family-based, personalized program assess effects of management on 186 clinical setting. Using mass spectrometry-based lipidomics, report an increase ceramides, alongside decrease lysophospholipids omega-3 fatty acids metabolism....
Background & AimsIndividual risk for developing alcohol-related liver disease (ALD) varies greatly. We hypothesized that metabolic factors and genetic polymorphisms predict severity of ALD.MethodsBiopsy-controlled, cross-sectional study in patients with a history excessive drinking. measured the homeostatic model assessment insulin resistance (HOMA-IR), plasma triglycerides, high- low-density lipoproteins (HDL, LDL), total cholesterol. Moreover, we genotyped four single nucleotide PNPLA3...
Typhoid Vi vaccines have been shown to be efficacious in children living endemic regions; however, a widely accepted correlate of protection remains established. We applied systems serology approach identify Vi-specific serological correlates using samples obtained from participants enrolled an experimental controlled human infection study. Participants were vaccinated with Vi-tetanus toxoid conjugate (Vi-TT) or unconjugated Vi-polysaccharide (Vi-PS) and subsequently challenged Salmonella...
Purpose.: To investigate the effects of T-cell–derived cytokines on gene and protein expression chemokines in a human RPE cell line (ARPE-19). Methods.: We used an vitro coculture system which CD3/CD28–activated T-cells were separated by membrane. chemokine genes was quantified using three different types microarrays. Protein determined single multiplex ELISA immunoblotting. Results.: Coculture with activated increased mRNA CCL2 (MCP-1); CCL5 (RANTES); CCL7 (MCP-3); CCL8 (MCP-2); CXCL1...
Through generation of the DEVoC, we added numerous previously uncharacterized viral genomes and genes to ever-increasing worldwide pool human gut viromes. The largest virome catalog generated from consistently processed fecal samples, facilitated analysis 91 healthy Danish
Abstract Background Frequent binge drinking is a known contributor to alcohol‐related harm, but its impact on systemic and hepatic inflammation not fully understood. We hypothesize that changes in immune markers play central role adverse effects of acute alcohol intake, especially patients with early liver disease. Aim To investigate the intoxication inflammation‐related venous plasma people disease (ArLD), non‐alcoholic fatty (NAFLD) healthy controls. Methods Thirty‐eight participants (13...
Abstract The levels of specific proteins in human blood are the most commonly used indicators potential health-related problems 1 . Understanding genetic and other determinants plasma proteome can aid biomarker research drug development. Diverse factors including genetics, age, sex, body mass index (BMI), growth development puberty affect circulating 2–5 Affinity-based proteomics infer relationship between protein these at a large scale 6–10 Compared to methods, spectrometry (MS)-based...
Abstract The microbiota in individual habitats differ both relative composition and absolute abundance. While sequencing approaches determine only the abundances of taxa genes, experimental techniques for abundance determination are rarely applied to large-scale microbiome studies. Here, we developed a machine learning approach predict fecal microbial loads (microbial cells per gram) solely from data. Applied datasets (n = 34,539), demonstrate that load is major determinant gut variation...
Ectopic liver fat deposition, resulting from impaired subcutaneous adipose tissue expandability, may represent an age-dependent key feature linking low birth weight (LBW) with increased risk of type 2 diabetes (T2D). We examined whether presumably healthy early middle-aged, non-obese LBW subjects exhibit content, in is associated the severity dysmetabolic traits and finally such associations be confounded by genetic factors.Using 1H magnetic resonance spectroscopy, we measured hepatic...
Pediatric obesity is associated with multi-organ inflammation and an increased risk of cardiometabolic disease. To identify novel biomarkers early-onset obesity-related risk, we performed targeted proteomics, analyzing 149 proteins related to cardiovascular disease in a cross-sectional sample 2,377 children adolescents overweight/obesity 1,647 normal weight. In addition, analyzed 64 circulating inflammation-related 184 adolescents, who participated 1-year family-based management program. We...
Purpose To investigate the effects of inflammatory factors and oxidative stress on cell survival human retinal pigment epithelial (RPE) line, ARPE-19. Methods Confluent RPE cells were treated with peripheral blood mononuclear cells-conditioned medium (PCM), H2O2, NaIO3, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, or combinations these. Cell viability was determined by assays light microscopy. Effector molecules death investigated immunofluorescence microscopy flow cytometry....
Uveal melanoma (UM) is the most common primary intraocular tumor in adults and presence of infiltrating leucocytes associated with a poor prognosis. Little known how influence cells. The purpose this study was to investigate effect activated T cells on expression chemotactic cytokines UM Furthermore, we examined ability stimulated attract monocytes.We used an vitro coculture system which cell lines were cultured together, but separated by membrane. gene quantified using microarray. Protein...
It is of international priority to develop a vaccine against sexually transmitted Chlamydia trachomatis infections combat the continued global spread infection. The optimal immunization strategy still remains be fully elucidated. aim this study was evaluate strategies in nonhuman primate (NHP) model. Cynomolgus macaques ( Macaqua fascicularis ) were immunized following different multi-component prime-boost immunization-schedules and subsequently challenged with C. SvD lower genital tract....
Background Lectin‐like oxidized low‐density lipoprotein (ox‐LDL) receptor‐1 is a scavenger receptor for lipoprotein. In adults, higher soluble lectin‐like ox‐LDL (sLOX‐1) levels are associated with cardiovascular disease, type 2 diabetes, and obesity, but similar link in pediatric overweight/obesity remains uncertain. Methods Results Analyses were based on the cross‐sectional HOLBAEK Study, including 4‐ to 19‐year‐olds from an obesity clinic group body mass index >90th percentile (n=1815)...
Background & AimsInfections are frequent in patients with cirrhosis and worsen prognosis. We evaluated the incidence of infections their impact on decompensation death early alcohol-related liver disease (ALD) during long-term follow-up.MethodsProspective cohort study secondary care a history excess alcohol intake, no prior decompensation, biopsies along clinical investigations conducted at baseline. During follow-up, we reviewed patients' electronic healthcare records for cases infections,...