A5081146253- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- Microscopic Colitis
- Herbal Medicine Research Studies
- interferon and immune responses
- Pediatric health and respiratory diseases
- Extracellular vesicles in disease
- Adipose Tissue and Metabolism
- Systemic Lupus Erythematosus Research
- ECG Monitoring and Analysis
- Microbial Community Ecology and Physiology
- Explainable Artificial Intelligence (XAI)
- Machine Learning in Healthcare
- Genomics and Phylogenetic Studies
- Nutritional Studies and Diet
- Infant Health and Development
- Obesity, Physical Activity, Diet
- Data Visualization and Analytics
- Advanced Proteomics Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Diet, Metabolism, and Disease
- Muscle metabolism and nutrition
- Infant Nutrition and Health
- Liver Disease Diagnosis and Treatment
- Respiratory and Cough-Related Research
Novo Nordisk Foundation
2023-2025
University of Copenhagen
2023-2025
European Molecular Biology Laboratory
2023-2024
Heidelberg University
2022-2023
European Molecular Biology Laboratory
2021-2023
Fecal microbiota transplantation (FMT) is a therapeutic intervention for inflammatory diseases of the gastrointestinal tract, but its clinical mode action and subsequent microbiome dynamics remain poorly understood. Here we analyzed metagenomes from 316 FMTs, sampled pre post intervention, treatment ten different disease indications. We quantified strain-level 1,089 microbial species, complemented by 47,548 newly constructed metagenome-assembled genomes. Donor strain colonization recipient...
Importance The effects of probiotic interventions on colonization with resistant bacteria and early microbiome development in preterm infants remain to be clarified. Objective To examine the efficacy Bifidobacterium longum subsp infantis , animalis lactis (BB-12), Lactobacillus acidophilus (La-5) probiotics prevent multidrug-resistant organisms or highly epidemic (MDRO+) shape toward eubiotic state healthy full-term infants. Design, Setting, Participants multicenter, double-blinded,...
Abstract Summary Taxonomic analysis of microbial communities is well supported at the level species and strains. However, can contain significant phenotypic diversity strains are rarely widely shared across global populations. Stratifying between identify ‘subspecies’, which a useful intermediary. High-throughput identification profiling subspecies not yet in microbiome field. Here, we use an operational definition based on single nucleotide variant (SNV) patterns within to profile...
Pediatric obesity is associated with multi-organ inflammation and an increased risk of cardiometabolic disease. To identify novel biomarkers early-onset obesity-related risk, we performed targeted proteomics, analyzing 149 proteins related to cardiovascular disease in a cross-sectional sample 2,377 children adolescents overweight/obesity 1,647 normal weight. In addition, analyzed 64 circulating inflammation-related 184 adolescents, who participated 1-year family-based management program. We...
The genetic regulation of the plasma proteome has been extensively studied in adult populations, yet protein quantitative trait loci (pQTL) studies children and adolescents remain largely unexplored. Here, we mapped pQTLs for 178 proteins measured using affinity-based proteomics 3,853 Danish (44.1% boys; median age 11.6 years) from HOLBAEK Study. We aimed to identify context-dependent pQTLs, where variant-protein associations varied across biological contexts such as obesity, puberty, sex....
Liver-generated plasma Apolipoprotein E (ApoE)-containing lipoproteins (LPs) (ApoE-LPs) play central roles in lipid transport and metabolism. Perturbations of ApoE can result several metabolic disorders genotypes have been associated with multiple diseases. is synthesized at the endoplasmic reticulum transported to Golgi apparatus for LP assembly; however, ApoE-LPs pathway from there membrane largely unknown. Here, we established an integrative imaging approach based on a fully functional...
Automatic analysis of colocalizing biological structures in multi-channel fluorescence microscopy images is an important task to quantify and understand processes at high spatial-temporal resolution. Here, we introduce a software suite for colocalization spot-like objects images. The consists ColocQuant ColocJ, easy use biologists. Python-based with graphical user interface particles two or three channels. Object-based performed by efficient multi-dimensional graph-based <inline-formula...
Deep neural networks are complex machine learning models that have shown promising results in analyzing high-dimensional data such as those collected from medical examinations. Such the potential to provide fast and accurate diagnoses. However, high complexity makes deep their predictions difficult understand. Providing model explanations can be a way of increasing understanding "black box" building trust. In this work, we applied transfer develop network predict sex electrocardiograms....
Abstract Although thousands of genetic variants are linked to human traits and diseases, the underlying mechanisms influencing these remain largely unexplored. One important aspect is understand how proteins regulated by genome identifying protein quantitative trait loci (pQTLs). Beyond this, there a need role complex genetics effects such as dominance epistasis that regulate plasma biomarkers. Therefore, we developed EIR-auto-GP, deep learning-based approach, identify effects. Our results...
Abstract Clostridioides difficile is an urgent threat in hospital-acquired infections world-wide, yet the microbial composition associated with C. , particular infection (CDI) cases, remains poorly characterised. To investigate gut microbiome CDI patients, we analysed 534 metagenomes from 10 publicly available study populations. We then tracked on a global scale, screening 42,900 253 public studies. Among cohorts, detected only 30% of stool samples patients. However, found that multiple...
Clostridioides difficile is an urgent threat in hospital-acquired infections world-wide, yet the microbial composition associated with C. , particular infection (CDI) cases, remains poorly characterised. To investigate gut microbiome CDI patients, we analysed 534 metagenomes from 10 publicly available study populations. We then tracked on a global scale, screening 42,900 253 public studies. Among cohorts, detected only 30% of stool samples patients. However, found that multiple other...
Clostridioides difficile is an urgent threat in hospital-acquired infections world-wide, yet the microbial composition associated with C. , particular infection (CDI) cases, remains poorly characterised. To investigate gut microbiome CDI patients, we analysed 534 metagenomes from 10 publicly available study populations. We then tracked on a global scale, screening 42,900 253 public studies. Among cohorts, detected only 30% of stool samples patients. However, found that multiple other...
Abstract Liver-generated plasma Apolipoprotein E (ApoE)-containing lipoproteins (LPs) (ApoE-LPs) play central roles in lipid transport and metabolism. Perturbations of ApoE can result several metabolic disorders genotypes have been associated with multiple diseases. is synthesized at the endoplasmic reticulum transported to Golgi apparatus for LP assembly; however, ApoE-LPs from there membrane largely unknown. Here, we established an integrative imaging approach based on a fully functional...