A5035693849- RNA Research and Splicing
- Microfluidic and Bio-sensing Technologies
- Nuclear Structure and Function
- Microbial Inactivation Methods
- Laser-Plasma Interactions and Diagnostics
- Cellular Mechanics and Interactions
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Laser-Matter Interactions and Applications
- Force Microscopy Techniques and Applications
- Laser-induced spectroscopy and plasma
- 3D Printing in Biomedical Research
- Conducting polymers and applications
- Protein Structure and Dynamics
- Advanced biosensing and bioanalysis techniques
- High-pressure geophysics and materials
- Microfluidic and Capillary Electrophoresis Applications
- Magnetic and Electromagnetic Effects
- Pulsed Power Technology Applications
- Electrohydrodynamics and Fluid Dynamics
- Advanced Sensor and Energy Harvesting Materials
- Surface Modification and Superhydrophobicity
- Rheology and Fluid Dynamics Studies
- Advanced Materials and Mechanics
- Plant Stress Responses and Tolerance
Wuhan University
2025
Johannes Gutenberg University Mainz
2019-2024
Institute of Molecular Biology
2019-2024
Fudan University
2024
Zhongshan Hospital
2024
State Key Laboratory of Genetic Engineering
2024
European Molecular Biology Laboratory
2020-2024
Nanjing University of Posts and Telecommunications
2024
China Academy of Engineering Physics
2020-2024
University of Applied Sciences Mainz
2022
Computed Tomography (CT) is a powerful method for non-destructive testing (NDT) and metrology awakes with expanding application fields. To improve the spatial resolution of high energy CT, micro-spot gamma-ray source based on bremsstrahlung from laser wakefield accelerator was developed. A CT using performed, which shows that reconstruction can reach 100 μm at 10% contrast. Our proof-of-principle demonstration indicates driven sources provide prospective way to increase toward micro CT. Due...
Abstract The approximately 120 MDa mammalian nuclear pore complex (NPC) acts as a gatekeeper for the transport between nucleus and cytosol 1 . central channel of NPC is filled with hundreds intrinsically disordered proteins (IDPs) called FG-nucleoporins (FG-NUPs) 2,3 Although structure scaffold has been resolved in remarkable detail, actual machinery built up by FG-NUPs—about 50 MDa—is depicted an 60-nm hole even highly tomograms and/or structures computed artificial intelligence 4–11 Here...
Small-molecule fluorophores enable the observation of biomolecules in their native context with fluorescence microscopy. Specific labeling via bio-orthogonal tetrazine chemistry combines minimal label size rapid kinetics. At same time, fluorogenic tetrazine–dye conjugates exhibit efficient quenching dyes prior to target binding. However, live-cell compatible long-wavelength strong fluorogenicity have been difficult realize. Here, we report close proximity distance between and fluorophore....
Theoretical analysis and experimental quantification on the ellipsoidal relaxation of vesicles are presented. The current work reveals simplicity universal aspects this process. Helfrich formula is shown to apply dynamic moderate-to-high tension membranes, a closed-form solution derived which predicts vesicle aspect ratio as function time. Scattered data unified by time scale, leads similarity behavior, governed distinctive for each type. Two separate regimes in identified, namely,...
Significance Despite emerging breakthroughs in the achievement of numerous elegant biomimetic structures that impart fascinating functionalities, bioinspired materials still suffer from poor structural durability, chemical reliability, flexibility, and optical transparency, as well unaffordable cost low throughput, thus preventing their broad real-life applications. In striking contrast to conventional wisdom, we demonstrate usually avoided detrimental elastic crack phenomenon can be...
Droplet microfluidics, which involves micrometer-sized emulsion droplets on a microfabricated platform, has been demonstrated as unique system for many biological and chemical applications. Robust scalable generation of monodisperse at high throughput is fundamental importance droplet microfluidics. Classic designs employ shear fluid dynamics to induce the breakup in two-phase flow size sensitive rate fluctuations, often resulting polydispersity. In this paper, we show spontaneous...
Abstract This study proposes a novel method utilizing subsonic flow fields to enhance combined laser-induced plasma shockwaves, achieving non-destructive and highly efficient removal of composite paint layers from 7075 aluminum alloy surfaces. A two-dimensional numerical model was established systematically investigate the influence mechanisms gas velocity lateral blowing angle on shockwave characteristics within fields. Time-resolved projection techniques were employed observe dynamic...
Dispersal of glioblastoma (GBM) cells leads to recurrence and poor prognosis. Accordingly, molecular pathways involved in dispersal are potential therapeutic targets. The mitogen activated protein kinase/extracellular signal regulated kinase (MAPK/ERK) pathway is commonly dysregulated GBM, targeting this with MEK inhibitors has proven effective controlling tumor growth. Since also regulates ECM remodeling actin organization − processes crucial cell adhesion, substrate attachment, motility –...
A novel viscosity measurement system based on a miniature 3D printed parallelogram flexure hinge structure and fiber-optic sensor is developed. Lorentz forces are applied to excite the vibration at various frequencies, used measure amplitude phase responses without electromagnetic interference. The modeled as single oscillator by taking into account liquid-induced mass damping well additive stiffness due liquid viscoelasticity. By curve fitting of experimentally obtained analytical model,...
Intrinsically disordered proteins (IDPs) adopt ensembles of rapidly fluctuating heterogeneous conformations, influencing their binding capabilities and supramolecular transitions. The primary conformational descriptors for understanding IDP ensembles—the radius gyration ( R G ), measured by small-angle X-ray scattering (SAXS), the root mean square (rms) end-to-end distance E probed fluorescent resonance energy transfer (FRET)—are often reported to produce inconsistent results regarding...
The mechanical behavior and cortical tension of single cells are analyzed using electrodeformation relaxation. Four types cells, namely, MCF-10A, MCF-7, MDA-MB-231, GBM, studied, with pulse durations ranging from 0.01 to 10 s. Mechanical response in the long-pulse regime is characterized by a power-law behavior, consistent soft glassy rheology resulting unbinding events within cortex network. In subsecond short-pulse regime, timescale well describes process indicates naive tensioned...
Abstract Electrically conductive nanocomposite elastomers are widely used in wearable electronics and for monitoring personal health owing to their great stretchability flexibility. However, working as interconnectors transmit signals, the elastomer is limited due large resistance variation under deformation that results inaccuracy reliability issues of whole system. Herein, a new technology employing Miura‐ori metastructure, together with surface buckling structure, into system fabricate...
Abstract Small-molecule fluorophores enable the observation of biomolecules in their native context with fluorescence microscopy. Specific labelling via bioorthogonal tetrazine chemistry confers minimal label size and rapid kinetics. At same time, fluorogenic tetrazine-dye conjugates exhibit efficient quenching dyes prior to target binding. However, live-cell compatible long-wavelength strong fluorogenicity have been difficult realize. Here, we report close proximity distance between...
Abstract HDAC8, a member of class I HDACs, plays pivotal role in cell cycle regulation by deacetylating the cohesin subunit SMC3. While cyclins and CDKs are well-established regulators, our knowledge other regulators remains limited. Here we reveal acetylation K202 HDAC8 as key regulator responsive to stress. primarily catalyzed Tip60, restricts activity, leading increased SMC3 arrest. Furthermore, cells expressing mutant form mimicking display significant alterations gene expression,...
Macromolecular crowding agents, such as poly(ethylene glycol) (PEG), are often used to mimic cellular cytoplasm in protein assembly studies. Despite the perception that agents have an inert nature, we demonstrate and quantitatively explore diverse effects of PEG on phase separation maturation condensates. We use two model proteins, FG domain Nup98 bovine serum albumin (BSA), which represent intrinsically disordered a with well-established secondary structure, respectively. expedites Nup98,...