A5022030142- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Electrohydrodynamics and Fluid Dynamics
- Neuroscience and Neural Engineering
- 3D Printing in Biomedical Research
- Electrowetting and Microfluidic Technologies
- Advancements in Transdermal Drug Delivery
- Cellular Mechanics and Interactions
- Microbial Inactivation Methods
- Analytical Chemistry and Sensors
- Dermatology and Skin Diseases
- Nanofabrication and Lithography Techniques
- Mechanical Circulatory Support Devices
- Electrochemical sensors and biosensors
- Mass Spectrometry Techniques and Applications
- Transgenic Plants and Applications
- Congenital Heart Disease Studies
- Muscle activation and electromyography studies
- Pluripotent Stem Cells Research
- Power Transformer Diagnostics and Insulation
- Advanced Sensor and Energy Harvesting Materials
- Urticaria and Related Conditions
- Spectroscopy Techniques in Biomedical and Chemical Research
- Asthma and respiratory diseases
Rutgers, The State University of New Jersey
2016-2025
Regeneron (United States)
2023-2024
Rutgers Sexual and Reproductive Health and Rights
2015-2024
Icahn School of Medicine at Mount Sinai
2018
Barro Colorado Island
2018
John Wiley & Sons (United States)
2018
Hudson Institute
2018
Penn State Milton S. Hershey Medical Center
2010
Pennsylvania State University
2003-2006
University of California, Berkeley
2000-2005
A microfluidic device for continuous, real time blood plasma separation is introduced. The principle of the from cells supported by Zweifach–Fung effect and was experimentally demonstrated using simple microchannels. composed a inlet, bifurcating region which leads to purified outlet, concentrated cell outlet. It designed separate an initial sample up 45% inlet hematocrit (volume percentage cells). network analogous electrical circuit, as well analytical numerical studies. functionality this...
A method for integrating porous polymer membranes such as polycarbonate, polyethersulfone and polyethylene terephthalate to microfluidic devices is described. The use of 3-aminopropyltriethoxysilane a chemical crosslinking agent was extended integrate with PDMS glass channels. strong, irreversible bond between the structure achieved. bonding strength in APTES treated significantly greater than fabricated using either "glue" or two-part epoxy method. Evaluation filtering microdevice pore via...
Abstract Electroporation is an electro-physical, non-viral approach to perform DNA, RNA, and protein transfections of cells. Upon application electric field, the cell membrane compromised, allowing delivery exogenous materials into Cell viability electro-transfection efficiency (eTE) are dependent on various experimental factors, including pulse waveform, vector concentration, type/density, electroporation buffer properties. In this work, effects composition eTE were systematically explored...
Genetic instability is promoted by unusual sequence arrangements and DNA structures. Hairpin structures can form from palindromes triplet repeats, they are also intermediates in V(D)J recombination. We have measured the genetic stability of a large palindrome which has potential to one-stranded hairpin or two-stranded cruciform structure analyzed recombinants at molecular level. A 15.3 kb introduced as transgene was found be transmitted normal Mendelian ratio mice, striking contrast profound...
This report describes the design, fabrication, and testing of a cross-flow filtration microdevice, for continuous extraction blood plasma from circulating whole sample in clinically relevant environment to assist monitoring patient's inflammatory response during cardiac surgeries involving cardiopulmonary bypass (CPB) procedures (about 400 000 adult 20 pediatric patients United States per year). The microfiltration system consists two-compartment mass exchanger with two aligned sets PDMS...
Background: The main concern in noninvasive (NI) glucose measurement is achieving high accuracy readings, although no blood (or other fluid) involved the process. Using methods based on different physical properties of a measured object can ensure independence each readings and therefore improve validity end result. By using combination (three) independent technologies—ultrasonic, electromagnetic, thermal—GlucoTrack™ presents unique approach for real-time, truly NI spot measurement. Methods:...
A transient analysis to quantify droplet deformation under DC electric fields is presented. The full Taylor-Melcher leaky dielectric model employed where the charge relaxation time considered be finite. assumed spheroidal in shape for all times. main result an ODE governing evolution of aspect ratio. validated by extensively comparing predicted with both previous theoretical and numerical studies, experimental data. Furthermore, effects parameters stresses on characteristics are...
Flow cytometry is an invaluable tool utilized in modern biomedical research and clinical applications requiring high throughput, resolution particle analysis for cytometric characterization and/or sorting of cells particles as well analyzing results from immunocytometric assays. In recent years, has focused on developing microfluidic flow cytometers with the motivation creating smaller, less expensive, simpler, more autonomous alternatives to conventional cytometers. These devices could...
Electrospray deposition (ESD) is a promising technique for depositing micro-/nano-scale droplets and particles with high quality repeatability. It particularly attractive surface coating of costly delicate biomaterials bioactive compounds. While efficiency ESD has only been successfully demonstrated spraying surfaces larger than the spray plume, this work extends its utility to smaller surfaces. shown that by architecting local "charge landscape", coatings plume size can be achieved....
Synapse formation analyses can be performed by imaging and quantifying fluorescent signals of synaptic markers. Traditionally, these are done using simple or multiple thresholding segmentation approaches labor-intensive manual analysis a human observer. Here, we describe Intellicount, high-throughput, fully-automated synapse quantification program which applies novel machine learning (ML)-based image processing algorithm to systematically improve region interest (ROI) identification over...
Suction-mediated cutaneous DNA uptake yielded high in vivo efficiency and thus provides an alternative transfection platform.
Proper brain function relies on the precise arrangement and flow of information between diverse neural subtypes. Developing improved human cell-based models which faithfully mimic biologically relevant connectivity patterns may improve drug screening efforts given limited success animal to predict safety efficacy therapeutics in clinical trials. To address this need, we have developed experimental defined circuitries through compartmentalization neuronal cell subtypes a 96 well plate-based...
Electrospray deposition (ESD) applies a high voltage to liquids flowing through narrow capillaries produce monodisperse generations of droplets down hundreds nanometers in diameter, each carrying small amount the delivered solute. This method has been combined with insulated stencil masks for fabricating micropatterns by spraying solutions containing nanoparticles, polymers, or biomaterials. To optimize fabrication process micro-coatings, self-limiting electrospray (SLED) recently developed....