A5100677207- Microbial Metabolic Engineering and Bioproduction
- Plant biochemistry and biosynthesis
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Retinal Diseases and Treatments
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Microbial Natural Products and Biosynthesis
- Underwater Acoustics Research
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Retinal Imaging and Analysis
- Ginseng Biological Effects and Applications
- Liver Disease Diagnosis and Treatment
- Kruppel-like factors research
- Steroid Chemistry and Biochemistry
- Fibroblast Growth Factor Research
- Connective Tissue Growth Factor Research
- biodegradable polymer synthesis and properties
- Distributed Control Multi-Agent Systems
- Energy Efficient Wireless Sensor Networks
- Lipid metabolism and biosynthesis
- Biopolymer Synthesis and Applications
- Indoor and Outdoor Localization Technologies
- Pharmacological Effects of Natural Compounds
Central Hospital of Zibo
2023-2025
Qingdao Institute of Bioenergy and Bioprocess Technology
2013-2024
Chinese Academy of Sciences
2012-2024
Northwestern Polytechnical University
2023-2024
Hebei Medical University
2023
Fourth Hospital of Hebei Medical University
2023
Tang Du Hospital
2023
Shandong Provincial Hospital
2023
Qingdao University of Science and Technology
2021-2023
Qinghai New Energy (China)
2023
Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. In our previous study, pathway for P3HP production was constructed in recombinant Esecherichia coli. Seven exogenous genes synthesis were carried by two plasmid vectors. However, the severely suppressed strain instability due to loss. this paper, strategies, chromosomal gene integration addiction system (PAS) based on amino acid anabolism, applied construct genetically stable strain. Finally, combination of...
Purpose This study aimed to evaluate the efficacy and safety of intravitreal injection aflibercept biosimilar in treatment diabetic macular edema (DME). Methods Clinical data were collected from 33 patients (40 eyes) newly diagnosed with DME ophthalmology department our hospital between February April 2024, all whom treated biosimilar. Patients managed according 3+ Pro re nata (PRN) regimen completed a minimum follow-up period 6 months. The best-corrected visual acuity (BCVA) testing,...
Exosomes are nanoscale vesicles shed from all cell types and play a major role in communication transportation of materials between cells due to their ability transfer proteins nucleic acids one another. Analogous size function synthetic nanoparticles, exosomes offer many advantages, rendering them the most promising candidates for targeted drug or gene delivery vehicles. can also induce chemoresistance radioresistance tumor cells. Studies about related mechanisms help overcome cancer...
Lycopene is increasing in demand due to its widespread use the pharmaceutical and food industries. Metabolic engineering synthetic biology technologies have been widely used overexpress heterologous mevalonate pathway lycopene Escherichia coli produce lycopene. However, tedious metabolic pathways complicated background, optimizing using reasonable design approaches becomes difficult.In this study, was introduced into E. divided three modules, with DMAPP serving as connecting nodes. The...
The mechanisms underlying the pathogenesis of steatosis and insulin resistance in nonalcoholic fatty liver disease remain elusive. Increased phosphorylation hepatic p38 has long been noticed liver; however, whether activation is a cause or consequence unclear. Here, we demonstrate that by MKK6 overexpression mice induces severe steatosis, reduces fat mass, elevates circulating acid levels p38α- FGF21-dependent manner. Mechanistically, through increasing FGF21 production from liver, increases...
This study aims to identify potential myopia biomarkers using machine learning algorithms, enhancing diagnosis and prognosis prediction.GSE112155 GSE15163 datasets from the GEO database were analyzed. We used "limma" for differential expression analysis "GO plot" "clusterProfiler" functional pathway enrichment analyses. The LASSO SVM-RFE algorithms employed screen myopia-related biomarkers, followed by ROC curve diagnostic performance evaluation. Single-gene GSEA was executed 4.1.0.The of...
Abstract Background ( R )-(+)-perillyl alcohol is a naturally oxygenated monoterpene widely used as the natural flavor additives, insecticides, jet fuels and anti-cancer therapies. It was also readily available precursors. However, this product present at low concentrations from plant sources which are not economically viable. Therefore, alternative microbial production methods rapidly emerging an attractive to make more sustainable environmentally friendly. Results We engineered Escherichia...
2,4-Dihydroxybutyric acid production via a methanol assimilation pathway is green and eco-friendly process without carbon loss.
(R)-(+)-perillyl alcohol is widely used in agricultural and anticarcinogenic fields. Microbial production of was investigated this study. We optimized biosynthesis Escherichia coli by using neryl pyrophosphate synthase NADPH regeneration. Engineering (NPP)-supplied pathway resulted a 4-fold improvement titer. Subsequently, combined engineering p-cymene monooxygenase (CymA) expression module for regeneration exhibited 15.4-fold increase titer over the initial strain S02. Finally, 453 mg/L...
(R)-(+)-perillyl alcohol is a much valued supplemental compound with wide range of agricultural and pharmacological characteristics. The aim this study was to improve production using whole-cell catalytic formula. In study, we employed plasmids varying copy numbers identify an appropriate strain, strain 03. We demonstrated that low levels alKL provided maximal biocatalyst stability. Upon determination the optimal conditions, yield reached 130 mg/L. For cofactor regeneration, constructed 10,...
Monocytes/macrophages play crucial roles in immunity to microorganisms and are one of the important targets for human immunodeficiency virus (HIV) infection. The phenotypes function monocytes HIV-infected patients were poorly determined. We herein detected expression Th1/Th2 cytokine receptors on monocyte subsets untreated either long term nonprogressor (LTNP) or chronic infection (CHI). CD14+CD16- significantly increased CD14+CD16+ reduced LTNP CHI compared with healthy control. IL-6R...
As to the problem of limited node power in wireless sensor networks, a new energy efficient distributed kalman filtering algorithm is proposed based on consensus given by Olfati-Saber and site percolation model— kind random graph. In this algorithm, each cluster contains with cluster-head some member nodes where used fuse data which come from process between neighbor cluster-head. Then, convergence proved applying Lyapunov second method matrix theory. At last, simulation example show that...
BackgroundProliferative diabetes retinopathy (PDR) seriously affects the vision of patients. Exploring key genes retinal neovascularization is crucial for developing new biomarkers and therapeutic targets.ObjectiveThis study aimed to identify associated with in Proliferative Diabetic Retinopathy (PDR), intending develop targets. This would further our understanding progression diabetic improve patient prognosis.MethodsThe gene data from 36 samples 45 healthy volunteers or patients were...