A5100768136- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Helicobacter pylori-related gastroenterology studies
- Gastrointestinal disorders and treatments
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Esophageal and GI Pathology
- Galectins and Cancer Biology
- Complement system in diseases
- Antibiotic Resistance in Bacteria
- Genomics and Chromatin Dynamics
- Peptidase Inhibition and Analysis
- Probiotics and Fermented Foods
- Pancreatic and Hepatic Oncology Research
- Pneumonia and Respiratory Infections
- RNA and protein synthesis mechanisms
- HIV Research and Treatment
- Microbial Metabolites in Food Biotechnology
- Antimicrobial Peptides and Activities
- Microscopic Colitis
- Blood groups and transfusion
- Renal Diseases and Glomerulopathies
- Brucella: diagnosis, epidemiology, treatment
- Infections and bacterial resistance
- Bacteriophages and microbial interactions
Hospital Universitario Virgen del Rocío
2024
Istituto Superiore di Sanità
1994-2023
Hospital Universitario de Móstoles
2008-2021
Universidad de Los Andes
2021
Hôpital Militaire Moulay Ismail
2020
York District Hospital
2020
University of Puerto Rico at Bayamón
2018
University of Chicago
2013-2014
Hospital Clínico San Carlos
2002-2010
University of Illinois Chicago
2003-2006
Risk factors for gastric cancer (GC) include inter-individual variability in the inflammatory response to Helicobacter pylori infection, ability of detoxifying DNA reactive species and repairing damage generated by oxidative stress dietary carcinogens. To evaluate association between polymorphic repair genes GC risk, a case-control study including 314 histologically confirmed patients 548 healthy controls was conducted high-risk area Tuscany, Italy. Polymorphic variants base excision...
It is uncertain whether Helicobacter pylori associated with ischemic syndromes and this association mediated by the induction of atherosclerosis. In study, we tested hypothesis that atherosclerotic stroke shows a selective virulent H strains.The seroprevalence infection strains bearing cytotoxin-associated gene-A (CagA), strong virulence factor, was assessed ELISA in 138 patients large-vessel (group A), 61 cardioembolic B), 151 healthy control subjects. The 3 groups had similar socioeconomic...
Bacterial genes defining intrinsic resistance to antibiotics encode proteins that can be targeted by antibiotic potentiators. To find such genes, a transposon insertion library of Acinetobacter baylyi was screened with subinhibitory concentrations various supersusceptible mutants. A DNA microarray printer used replica plate 10,000 individual clones select mutants unable grow at 1/10 the MICs 12 different antibiotics. Transposon insertions in 11 were found cause an eightfold or higher...
A 65 kDa-constituent (MP65) of a whole-cell mannoprotein (MP) fraction Candida albicans was purified by immunoaffinity chromatography with monoclonal antibodies directed against periodate-insensitive, protease-sensitive MP epitopes, putatively polypeptide in nature. These were obtained immunization mice concanavalin bead-coupled, low-glycosylated from hyphal cells C. grown the presence subinhibitory dose tunicamycin. The immunoaffinity-purified MP65 molecule had pI 4.1 and...
Host genetic factors, including the IL1 gene cluster, play a key role in determining long-term outcome of Helicobacter pylori infection. The aim study was to investigate relationship between selected loci polymorphisms and gastric cancer risk an Italian population.In case-control we compared IL1B-31 IL1B+3954 biallelic IL1RN pentaallelic variable number tandem repeats (VNTR) 185 patients 546 controls randomly sampled from general population area at high (Tuscany, Central Italy).Genotype...
The expression of cytokine genes in cultures human peripheral blood mononuclear cells (PBMC) stimulated with mannoprotein constituents (MP) Candida albicans has been studied by means S1 nuclease mapping analysis, polymerase chain reaction, and enzyme-linked immunosorbent assay. MP induced early, consistent, long-lasting production interleukin-1 beta (IL-1 beta), tumor necrosis factor alpha, IL-6 mRNAs. Similar results were obtained when the same PBMC purified protein derivative (PPD) from...
Abstract Glutathione S‐Transferases (GSTs) are a family of phase II enzymes involved in the detoxification potential carcinogens and provided strong antioxidant function by neutralizing electrophiles free radicals. The GSTM1 GSTT1 isoenzymes exhibit deletion polymorphisms, resulting lack activity, null genotypes have been associated with increased cancer risk at several sites, including stomach, although contrasting results. We carried out case‐control study to evaluate whether these...
Journal Article Identification of a 65-kDa Mannoprotein as Main Target Human CellMediated Immune Response to Candida albicans Get access Antonella Torosantucci, Torosantucci Laboratory Bacteriology and Medical Mycology, Istituto Superiore di Sanità, And Cenlro CNR Tipizzaione Tissutale e Problemi della Dialisi, Rome, Italy Search for other works by this author on: Oxford Academic PubMed Google Scholar Carla Bromuro, Bromuro Maria J. Gomez, Gomez Clara M. Ausiello, Ausiello Francesca Urbani,...
ABSTRACT A 65-kDa mannoprotein (CaMp65) has long been studied as a major, immunodominant antigen of the human opportunistic pathogen Candida albicans . An expression library C. was screened with serum from mice immunized ScMp65 (ScW10), Saccharomyces cerevisiae recombinant protein about 48 kDa. This recognized CaMp65 cell wall extract After cloning and sequencing relevant cDNA, an open reading frame encoding 379 amino acids identified. Its deduced acid sequence showed regions identity all...
Mucosal candidiasis is one of the first opportunistic diseases in HIV-infected subjects. In order to understand relationship between this disease and immunodeficiency chemically defined, immunodominant Candida antigens, a mannoprotein fraction from C. albicans cell wall (GMP) was used analyse proliferative non-MHC-restricted cytotoxic responses peripheral blood mononuclear cells (PBMC) normal former, GMP induced extensive blastogenesis, generation powerful cytotoxicity against tumour line...
ABSTRACT T-cell-mediated immunity is known to play a central role in the host response Candida albicans. T-cell clones are useful tools for exact identification of fungal epitopes and processing requirements C. albicans antigens. We isolated human from an HLA-DRB1*1101 healthy donor by using antigenic extract (MP-F2) fungus. Specific were receptor α/β CD4 + /CD8 − showed T-helper type 1 cytokine profile (production gamma interferon not interleukin-4). The large majority these recognized both...
The sodium dodecyl sulfate (SDS) extraction-trypsin digestion protocol used by Braun and Sieglin (V. U. Sieglin, Eur. J. Biochem. 13:336-346, 1970) to show the peptidoglycan-linked lipoprotein of Escherichia coli was applied both Brucella abortus E. coli. Whereas a single polypeptide 8,000 molecular weight obtained from coli, several proteins apparent lower than 35,000 were demonstrated SDS-polyacrylamide gel electrophoresis in B. abortus. These results did not change when trypsin conditions...
The release of mannoprotein (MP) antigen from Candida albicans grown at 28°C (yeast form) or 37°C (mycelial form), and the ability each released material to stimulate a cell-mediated immune (CMI) response by human lymphocytes in vitro, were studied. Overall, mycelial cells more MP per unit dry mass increase was relatively enriched with constituents lower molecular respect yeast cells. Moreover, contained 65 kDa component (MP65) which largely predominant recognized rabbit anti-mycelium...
We previously demonstrated that mannoprotein (MP) from Cryptococcus neoformans (CnMP) stimulates interleukin-12 production by human monocytes, thus fostering a T-helper type 1 (Th1) protective anticryptococcal response. In this paper we show CnMP was also able to induce Candida albicans-directed Th1 This for mice immunized with induction of delayed-type hypersensitivity (DTH) reaction C. albicans MP (CaMP) as well gamma interferon CD4(+) and CD8(+) splenic T cells stimulated in vitro CaMP....
Summary Aim : To test the hypothesis that duodenal colonization represents final crucial step in development of Helicobacter pylori related ulcer. Methods Patients with non‐ulcer dyspepsia who had gastric by H. were included study. At baseline endoscopy, we evaluated prevalence (culture, urease testing and histology), cytotoxin‐associated gene A status (polymerase chain reaction). No patients received eradication during 1 year follow‐up. this time, endoscopy was repeated incidence ulcer...
During incubation in vitro with yeast or germ tube forms of Candida albicans, only 2 to 6% freshly isolated human natural killer (NK) cells (> 85% CD16+, CD56+, CD3-; < 15% CD3+; cytolytic for the NK-susceptible target K562 but not NK-resistant DAUDI), were seen interact fungal cells. As under electron microscope, contact area had a limited extent and was narrow, neither surface nor intracytoplasmic organization NK cell altered. In contrast, more than 30% interleukin-2-activated (LAK)...
ABSTRACT In the search of antigenic determinants a 65-kDa mannoprotein (MP65) Candida albicans , tryptic fragments immunoaffinity-purified MP65 preparations were tested for their ability to induce lymphoproliferation human peripheral blood mononuclear cells (PBMC). Five major peptides (T1 T5) shown vigorous proliferation PBMC from majority eight healthy subjects tested. With use synthetic peptides, critical amino acid sequences two most immunoactive and T2) determined. Similar what was found...
Yeast or mycelial cultures of Candida albicans released comparable amounts Concanavalin A-reactive mannoprotein material after 24 h growth, and in both cases this showed a qualitatively similar SDS-PAGE pattern, with predominantly polydisperse constituents high molecular mass. The two secretion mixtures also reactivity by ELISA serum from subject titre anti-Candida antibodies, as well an hyperimmune antiserum raised rabbits. Both secreted extracts were separated ion-exchange chromatography...