Mercury is a toxic heavy metal widely dispersed in the natural environment. exposure induces an increase oxidative stress red blood cells (RBCs) through production of reactive species and alteration endogenous antioxidant defense system. Recently, among various antioxidants, polyphenols from extra-virgin olive oil (EVOO), important element Mediterranean diet, have generated growing interest. Here, we examined potential protective effects hydroxytyrosol (HT) and/or homovanillyl alcohol (HVA)...

The aim of this study was to evaluate changes in the chemical profile essential oils and antioxidant enzymes activity (catalase CAT, superoxide dismutase SOD, Glutathione S-transferases GST, Peroxidase POX) Mentha × piperita L. (Mitcham variety) arvensis (var. piperascens), response heat stress. In addition, we used salicylic acid (SA) melatonin (M), two brassinosteroids that play an important role regulating physiological processes, assess their potential mitigate both species, stress...

Metabolomics is a method that provides an overview of the physiological and cellular state specific organism or tissue. This particularly useful for studying influence environment can have on organisms, especially those used as bio-indicators, e.g., Mytilus galloprovincialis. Nevertheless, scarcity data complete metabolic baseline mussel tissues still exists, but more importantly, effect exposure to certain heavy metals spermatozoa unknown, also considering that, in recent years,...

Nickel is associated with reproductive toxicity, but little known about the molecular mechanisms of nickel-induced effects on sperm chromatin and protamine-like proteins (PLs). In present work, we analyzed PLs from Mytilus galloprovincialis by urea-acetic acid polyacrylamide gel electrophoresis (AU-PAGE) SDS-PAGE assessed their binding to DNA Electrophoretic Mobility Shift Assay (EMSA) after exposing mussels 5, 15, 35 µM NiCl2 for 24 h. addition, a time course digestion MNase release nuclei...

Here we report the industrial pollution effects due to cadmium on reproductive health of Mytilus galloprovincialis. Mussels were removed from biofouling a Conatex panel after one year exposition at polluted site near disposal metallurgical factory. A high bioaccumulation was observed in testis mussels housed site, with respect control as determined by inductively coupled plasma-mass spectrometry, along 10 fold increase metallothionein 20 kDa gene (mt20) expression levels qPCR. Furthermore,...

Plant polyphenols, with broadly known antioxidant properties, represent very effective agents against environmental oxidative stressors, including mercury. This heavy metal irreversibly binds thiol groups, sequestering endogenous antioxidants, such as glutathione. Increased incidence of food-derived mercury is cause for concern, given the many severe downstream effects, ranging from kidney to cardiovascular diseases. Therefore, possible beneficial properties Feijoa sellowiana toxicity were...

Mercury (Hg) is a global environmental pollutant that affects human and ecosystem health. With the aim of exploring Hg-induced protein modifications, intact erythrocytes were exposed to HgCl

Mercury (Hg) is an environmental pollutant that impacts human and ecosystem health. In our previous works, we reported alterations in the properties of Mytilus galloprovincialis protamine-like (PL) proteins after 24 h exposure to subtoxic doses toxic metals such as copper cadmium. The present work aims assess effects 1, 10, 100 pM HgCl2 on spermatozoa PL galloprovincialis. Inductively coupled plasma–mass spectrometry indicated accumulation this metal gonads exposed mussels. Further, RT-qPCR...

Mercury is one of the most dangerous environmental pollutants. In this work, we analysed effects exposure

In this paper we report for the first time a method extraction of protein fraction from Feijoa sellowiana Berg. fruit and its electrophoretic analysis. addition analyzed antioxidant activity effectiveness against different Gram-positive Gram-negative bacteria both as American type culture collection (ATCC) standard clinically isolated strains. Both antimicrobial resulted stronger respect to that previously obtained acetonic extract same fruit. Further, study activities also on product in...

Mercury (Hg) is a highly toxic and widespread pollutant. We previously reported that the exposure of Mytilus galloprovincialis for 24 h to doses HgCl2 similar those found in seawater (range 1-100 pM) produced alterations properties protamine-like (PL) proteins rendered them unable bind protect DNA from oxidative damage. In present work, deepen our studies, we analyzed PL by turbidimetry fluorescence spectroscopy performed salt-induced release analyses these sperm nuclei after mussels at same...

Phosphatidylserine (PS) translocation to the external membrane leaflet represents a key mechanism in pathophysiology of human erythrocytes (RBC) acting as an “eat me” signal for removal aged/stressed cells. Loss physiological asymmetry, however, can lead adverse effects on cardiovascular system, activating prothrombotic activity. The data presented indicate that structurally related olive oil phenols prevent cell alterations induced intact RBC exposed HgCl2 (5–40 µM) or Ca2+ ionophore (5...

Folk medicine uses wild herbs, especially from the Lamiaceae family, such as oregano and lavender, in treatment of many diseases. In present study, we investigated antibacterial activity essential oils Origanum glandulosum Desf. Lavandula dentata L. against multidrug-resistant Klebsiella pneumoniae strains. The chemical composition their effect on ultrastructure tested bacteria release cellular components that absorb at 260 nm were studied. Furthermore, cytotoxicity production reactive...

The major acid-soluble protein components of the mussel Mytilus galloprovincialis sperm chromatin consist protamine-like proteins PL-II, PL-III and PL-IV, an intermediate group nuclear basic between histones protamines. aim this study was to investigate bactericidal activity these since, date, there are reports on protamines histones, but not proteins. We tested against Gram-positive bacteria: Enterococcus faecalis two different strains Staphylococcus aureus, as well Gram-negative Proteus...

Background/Aims: Mercury (Hg) is a heavy metal widespread in all environmental compartments as one of the most hazardous pollutants. Human exposure to this natural element detrimental for several cellular types including erythrocytes (RBC) that accumulate Hg mainly bound SH groups different components, protein cysteine residues. The membrane represents major target Hg-induced damage RBC with loss physiological phospholipid asymmetry, due phosphatidylserine (PS) external leaflet. To...

In several physiopathological processes, phosphatidylserine (PS), normally sequestered to the inner leaflet of plasma membrane, becomes exposed cell surface. erythrocytes (RBC), PS externalization is a crucial event for removal aged/damaged cells but can also be associated with increased prothrombotic activity. Structurally related olive oil antioxidants, including hydroxytyrosol (HT), are able significantly reduce percentage PS-exposing RBC, when toxic compounds such as heavy metal mercury...

The functioning of the immune and reproductive systems is crucial for fitness survival species strongly influenced by environment. To evaluate effects short-term heat stress (HS) on these systems, confirming deepening previous studies, female sea urchin Paracentrotus lividus were exposed 7 days to 17 °C, 23 28 °C. Several biomarkers detected such as ferric reducing power (FRAP), ABTS-based total antioxidant capacity (TAC-ABTS), nitric oxide metabolites (NOx), thiol levels (TTL),...

Background/Aims: Lysophosphatidic acid (LPA) is a phospholipid signal molecule that regulates many cellular processes both physiological and pathological.Moreover, its high plasma concentrations are toxic for several types, including erythrocytes (RBC), as it acts pro-thrombotic pro-atherogenic agent.It therefore essential to explore the potential protective role of nutrition in protecting cells from possible effects LPA by testing bioactive nutrients.In particular, our focus was on...

The consumption of seafood has increased in recent years, especially coastal regions.The mussels provides proteins, essential minerals and vitamins, thus, some protection from certain diseases but the risks benefits their are still hard to assess because metals bioaccumulated marine environment, with toxicity.Mussels accumulate a wide range metals, included cadmium, soft tissue.Cadmium is heavy metal particularly hazardous for human health an important pollutant estuarine environments.Thus,...