A5016165669- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Neonatal Respiratory Health Research
- Chronic Myeloid Leukemia Treatments
- Occupational and environmental lung diseases
- Kruppel-like factors research
- Extracellular vesicles in disease
- Cancer-related gene regulation
- Tissue Engineering and Regenerative Medicine
- Telomeres, Telomerase, and Senescence
- Radiation Therapy and Dosimetry
- Helicobacter pylori-related gastroenterology studies
- Respiratory Support and Mechanisms
- Galectins and Cancer Biology
- Effects of Radiation Exposure
- Transplantation: Methods and Outcomes
- Macrophage Migration Inhibitory Factor
- Tracheal and airway disorders
- Congenital Diaphragmatic Hernia Studies
- Mesenchymal stem cell research
- Health, Environment, Cognitive Aging
- Advanced Radiotherapy Techniques
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Eosinophilic Disorders and Syndromes
- Pulmonary Hypertension Research and Treatments
German Center for Lung Research
2022-2025
Helmholtz Zentrum München
2019-2024
Ludwig-Maximilians-Universität München
2020-2021
LMU Klinikum
2021
Center for Environmental Health
2019
Universidad de Los Andes
2018
Idiopathic pulmonary fibrosis (IPF) is a lethal chronic lung disease characterized by aberrant intercellular communication, extracellular matrix deposition, and destruction of functional tissue. While vesicles (EVs) accumulate in the IPF lung, their cargo biological effects remain unclear. We interrogated proteome EV non-EV fractions during contribution to fibrosis. EVs accumulated 14 days after bleomycin challenge, correlating with decreased function initiated fibrogenesis healthy...
Aging is the main risk factor for chronic lung diseases (CLDs) including idiopathic pulmonary fibrosis (IPF) and obstructive disease (COPD). Accordingly, hallmarks of aging like cellular senescence are increased in these patients different cell types fibroblasts. However, little known about triggers that induce a phenotype backgrounds its role CLD pathogenesis. Therefore, we characterized primary human fibroblasts (phLF) from control, IPF, or COPD at baseline after exposure to...
Abstract Background Invasiveness is a major factor contributing to metastasis of tumour cells. Given the broad variety and plasticity invasion mechanisms, assessing potential metastasis-promoting effects irradiation for specific mechanisms important further understanding adverse radiotherapy. In fibroblast-led fibroblasts produce tracks in extracellular matrix which cancer cells with epithelial traits can follow. So far, influence on this type has not been assessed. Methods By...
Clinically relevant methods are not available that prioritize and validate potential therapeutic targets for individual tumors, from the vast amount of tumor descriptive expression data. We established inducible transgene in clinically patient-derived xenograft (PDX) models vivo to fill this gap. With technique at hand, we analyzed role transcription factor Krüppel-like 4 (KLF4) B-cell acute lymphoblastic leukemia (B-ALL) PDX different disease stages. In competitive preclinical trials, found...
Abstract Aging is the main risk factor for chronic lung diseases including idiopathic pulmonary fibrosis (IPF) and obstructive disease (COPD). Accordingly, hallmarks of aging such as cellular senescence are increased in different cell types fibroblasts lungs these patients. However, whether senescent phenotype derived from IPF or COPD differs still unknown. Therefore, we characterized at baseline after exposure to disease-relevant insults (H 2 O , bleomycin, TGF-β1) cultured primary human...
Abstract Aging is the main risk factor for chronic lung diseases (CLDs) including idiopathic pulmonary fibrosis (IPF) and obstructive disease (COPD). Accordingly, hallmarks of aging such as cellular senescence are present in different cell types fibroblasts these patients. However, whether senescent phenotype derived from IPF or COPD patients differs still unknown. Therefore, we characterized at baseline after exposure to disease-relevant insults (H 2 O , bleomycin, TGF-β1) cultured primary...
Cellular senescence is a major hallmark of aging. Senescence defined as an irreversible growth arrest observed when cells are exposed to variety stressors including DNA damage, oxidative stress, or nutrient deprivation. While well-established driver aging and age-related diseases, it highly heterogeneous process with significant variations across organisms, tissues, cell types. The relatively low abundance in healthy aged tissues represents challenge studying given organ, the human lung. To...
Objetivo: este estudio caracteriza la diversidad de los genes virulencia cagA (gen asociado con citotoxina A) y vacA (citotoxina vacuolizante) en pacientes colombianos para determinar posibles asociaciones entre estos 2 severidad hallazgos endoscópicos teniendo cuenta todos genotipos reportados el gen (s, m e i).Materiales métodos: Helicobacter pylori fue detectado por cultivo métodos moleculares biopsias 62 pacientes. Los (m/i/s) se determinaron reacción cadena polimerasa (PCR)...
Aging is a major risk factor for lung fibrosis. While bleomycin injury in young mice leads to resolving fibrosis, aged develop non-resolving resembling human disease. Here, we conducted longitudinal single-cell RNA profiling of (3 mo) and (18 after bleomycin-induced lung (days 0/3/10/20/30/37 p.i., n=55 mice), coupled multiplexed immunofluorescence uncover spatially-resolved cellular programs responsible this impaired regeneration. Single-cell analyses over 160,000 cells across 69 cell...
Clinic-close methods are not available that prioritize and validate potential therapeutic targets in individual tumors from the vast bulk of descriptive expression data. We developed a novel technique to express transgenes established patient-derived xenograft (PDX) models vivo fill this gap. With at hand, we analyzed role transcription factor Krüppel-like 4 (KLF4) B-cell acute lymphoblastic leukemia (B-ALL) PDX different disease stages. In competitive pre-clinical trials, found...