A5003508708- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Advanced Chemical Sensor Technologies
- Reproductive Biology and Fertility
- Mass Spectrometry Techniques and Applications
- Diet and metabolism studies
- Reproductive Physiology in Livestock
- Gut microbiota and health
- Advanced Proteomics Techniques and Applications
- Genetic Mapping and Diversity in Plants and Animals
- Allelopathy and phytotoxic interactions
- Insect and Pesticide Research
- Plant-Microbe Interactions and Immunity
- Photosynthetic Processes and Mechanisms
- Meat and Animal Product Quality
- Plant Parasitism and Resistance
- Wheat and Barley Genetics and Pathology
- Genetic and phenotypic traits in livestock
- Genetics and Plant Breeding
- Fermentation and Sensory Analysis
- Plant Stress Responses and Tolerance
- Bioinformatics and Genomic Networks
- Microbial Community Ecology and Physiology
- Liver Disease Diagnosis and Treatment
- Insect-Plant Interactions and Control
Colorado State University
2016-2025
Proteogenomics Research Institute for Systems Medicine
2010
Noble Research Institute
2004-2007
Collins College
2006
Virginia Tech
2002-2003
ABSTRACT Plants are in constant contact with a community of soil biota that contains fungi ranging from pathogenic to symbiotic. A few studies have demonstrated critical role chemical communication establishing highly specialized relationships, but the general for root exudates structuring fungal is poorly described. This study demonstrates two model plant species ( Arabidopsis thaliana and Medicago truncatula ) able maintain resident populations unable nonresident populations. mediated...
Summary The identification of leaf wax genes involved in stress tolerance is expected to have great potential for crop improvement. Here we report the characterization a novel AP2 domain‐containing putative transcription factor gene from model legume Medicago truncatula. gene, designated WXP1 , able activate w x p roduction and confer drought alfalfa ( sativa ), most important forage species world close relative M. truncatula . predicted protein has 371 aa; it one longest peptides all single...
GC-MS-based metabolite profiling was used to analyse the response of Medicago truncatula cell cultures elicitation with methyl jasmonate (MeJa), yeast elicitor (YE), or ultraviolet light (UV). Marked changes in levels primary metabolites, including several amino acids, organic and carbohydrates, were observed following MeJa. A similar, but attenuated YE elicitation, whereas little UV elicitation. MeJa induced accumulation triterpene beta-amyrin, a precursor saponins, LC-MS analysis confirmed...
Analyses of circulating metabolites in large prospective epidemiological studies could lead to improved prediction and better biological understanding coronary heart disease (CHD). We performed a mass spectrometry-based non-targeted metabolomics study for association with incident CHD events 1,028 individuals (131 events; 10 y. median follow-up) validation 1,670 (282 3.9 follow-up). Four were replicated independent main cardiovascular risk factors [lysophosphatidylcholine 18∶1 (hazard ratio...
Metabolomic data are frequently acquired using chromatographically coupled mass spectrometry (MS) platforms. For such datasets, the first step in analysis relies on feature detection, where a is defined by and retention time. While typically derived from single compound, spectrum of signals more more-accurate representation spectrometric signal for given metabolite. Here, we report novel grouping method that operates an unsupervised manner to group MS into spectra without relying...
A current and significant limitation to metabolomics is the large-scale, high-throughput conversion of raw chromatographically coupled mass spectrometry datasets into organized data matrices necessary for further statistical processing visualization. This article describes a new extraction tool, MET-IDEA (Metabolomics Ion-based Data Extraction Algorithm) which surmounts this void. compatible with diversity systems, generates an output similar traditional quantification methods, utilizes...
Abstract Following recent indirect evidence suggesting a role for ATP-binding cassette (ABC) transporters in root exudation of phytochemicals, we identified 25 ABC transporter genes highly expressed the cells most likely to be involved secretion processes. Of these genes, also selected six full-length and half-size in-depth molecular biochemical analyses. We compared exuded phytochemical profiles seven mutants those wild type. There were three nonpolar phytochemicals missing various type...
Abstract Background Chemical and biological processes dictate an individual organism's ability to recognize respond other organisms. A small but growing body of evidence suggests that plants may be capable recognizing responding neighboring in a species specific fashion. Here we tested whether or not individuals the invasive exotic weed, Centaurea maculosa , would modulate their defensive strategy response different plant neighbors. Results In greenhouse, C. were paired with either...
Emerging evidence supporting chronic disease fighting properties of rice bran has advanced the development stabilized for human use as a functional food and dietary supplement. A global targeted metabolomic investigation fermented with Saccharomyces boulardii was performed in three varieties. Metabolites from S. boulardii-fermented were detected by gas chromatography−mass spectrometry (GC−MS) assessed bioactivity compared to nonfermented normal malignant lymphocytes. Global metabolite...
Heat-stabilized rice bran (SRB) and cooked navy bean powder (NBP) contain a variety of phytochemicals that are fermented by colonic microbiota may influence intestinal health. Dietary interventions with these foods should be explored for modulating colorectal cancer risk.A randomized-controlled pilot clinical trial investigated the effects eating SRB (30 g/day) or (35 on gut metabolites (NCT01929122). Twenty-nine overweight/obese volunteers prior history consumed study-provided meal snack...
Metabolomics is an emerging method to improve our understanding of how genetic diversity affects phenotypic variation in plants. Recent studies have demonstrated that genotype has a major influence on biochemical several types plant tissues, however, the association between metabolic and morphological physiological traits largely unknown. Sorghum bicolor (L.) important food fuel crop with extensive variation. lines been bred for differing phenotypes beneficial production grain (food), stem...
Liquid chromatography coupled to electrospray ionization-mass spectrometry (LC-ESI-MS) is a versatile and robust platform for metabolomic analysis. However, while ESI soft ionization technique, in-source phenomena including multimerization, nonproton cation adduction, fragmentation complicate interpretation of MS data. Here, we report chromatographic mass spectrometric behavior 904 authentic standards collected under conditions identical typical nontargeted profiling experiment. The data...
Abstract Ambient mass spectrometry is an analytical approach that enables ionization of molecules under open-air conditions with no sample preparation and very fast sampling times. Rapid evaporative (REIMS) a relatively new type ambient has demonstrated applications in both human health food science. Here, we present evaluation REIMS as tool to generate molecular scale information objective measure for the assessment beef quality attributes. Eight different machine learning algorithms were...
Global wildfire activity has increased since the 1970s and is projected to intensify throughout 21st century. Wildfires change composition biodegradability of soil organic matter (SOM) which contains nutrients that fuel microbial metabolism. Though persistent forms SOM often increase postfire, response more biodegradable remains unclear. Here we simulated severe wildfires through a controlled "pyrocosm" approach identify sources characterize metabolome immediately postfire. Using amplicon...
• Gene expression in response to signaling molecules has been well studied the leaves of model plant species Arabidopsis thaliana. However, knowledge gene and metabolic regulation at root level is limited. Here, compounds salicylic acid (SA), methyl jasmonate (MeJA) nitric oxide (NO) were applied exogenously induce various defense responses roots, their effect was using a combination genomic, molecular biochemical approaches. Genes involved signaling/activation, cellular redox state,...
ABSTRACT Multidrug-resistant tuberculosis has emerged as a major threat to control. Phylogenetically related rifampin-resistant actinomycetes with mutations mapping clinically dominant Mycobacterium in the rpoB gene show upregulation of networks encoding secondary metabolites. We compared expressed proteomes and metabolomes two fully drug-susceptible clinical strains M. (wild type) those their respective rifampin-resistant, mutant progeny confirmed rifampin monoresistance following...
Heat-stabilized rice bran (SRB) has been shown to regulate blood lipids and glucose, modulate gut mucosal immunity inhibit colorectal cancer in animal human studies. However, SRB’s effects on microbial composition metabolism the resulting implications for health remain largely unknown. A pilot, randomized-controlled trial was developed investigate of eating 30 g/day SRB stool microbiome metabolome. Seven healthy participants consumed a study meal snack daily 28 days. The metabolome were...