A5015416028- Postharvest Quality and Shelf Life Management
- Plant Surface Properties and Treatments
- Genetic Mapping and Diversity in Plants and Animals
- Genetic and phenotypic traits in livestock
- Genetics and Plant Breeding
- Polysaccharides and Plant Cell Walls
- Plant Physiology and Cultivation Studies
- Plant Reproductive Biology
- Lipid metabolism and biosynthesis
- Horticultural and Viticultural Research
- Plant Molecular Biology Research
- Plant biochemistry and biosynthesis
- Plant nutrient uptake and metabolism
- Insect and Pesticide Research
- Tree Root and Stability Studies
- Genetic and Environmental Crop Studies
- Food composition and properties
- Legume Nitrogen Fixing Symbiosis
- Antioxidant Activity and Oxidative Stress
- Biofuel production and bioconversion
- Insect Resistance and Genetics
- Biological Control of Invasive Species
- Plant Gene Expression Analysis
- Isotope Analysis in Ecology
- Advanced Cellulose Research Studies
Ithaca College
2024
Cornell University
2009-2021
Robert W. Holley Center for Agriculture & Health
2018-2019
United States Department of Agriculture
2019
Energy Biosciences Institute
2016
University of California, Berkeley
2014-2016
University of British Columbia
2006-2010
Abstract Plant epidermal cells dedicate more than half of their lipid metabolism to the synthesis cuticular lipids, which seal and protect plant shoot. The cuticle is made up a cutin polymer waxes, diverse hydrophobic compounds including very-long-chain fatty acids derivatives. How such are exported cuticle, especially through hydrophilic cell wall, not known. By performing reverse genetic screen, we have identified LTPG, glycosylphosphatidylinositol-anchored transfer protein that highly...
Horizontal gene transfer (HGT) involves the nonsexual transmission of genetic material across species boundaries. Although often detected in prokaryotes, examples HGT involving animals are relatively rare, and any evolutionary advantage conferred to recipient is typically obscure. We identified a ( HhMAN1 ) from coffee berry borer beetle, Hypothenemus hampei , devastating pest coffee, which shows clear evidence bacteria. encodes mannanase, representing class glycosyl hydrolases that has not...
Tomato (Solanum lycopersicum) is the primary model for study of fleshy fruits, and research in this species has elucidated many aspects fruit physiology, development, metabolism. However, most these studies have involved homogenization pericarp, with its constituent cell types. Here, we describe coupling pyrosequencing technology laser capture microdissection to characterize transcriptomes five principal tissues pericarp from tomato fruits (outer inner epidermal layers, collenchyma,...
The majority of terrestrial biomass accumulates as plant cell walls, the main structural component leaves, stems, roots, fruits, and seeds. constituents walls are lignin polysaccharides, which can be transformed into liquid fuel molecules through chemical transformation or microbial fermentation. Because large scale demand for fuel, it is essential that biomass-to-fuel conversion processes maximize conservation energy in products. Here, we summarize some challenges posed to these by complexity walls.
The plant cuticle is thought to be a critical evolutionary adaptation that allowed the first plants colonize land, because of its key roles in regulating water status and providing protection from biotic abiotic stresses. Much has been learned about composition structure through genetic biochemical studies angiosperms, as well underlying pathways, but little known cuticles early diverging lineages. Here, we demonstrate moss Physcomitrella patens, an extant relative earliest terrestrial...
Summary The aerial epidermis of all land plants is covered with a hydrophobic cuticle that provides essential protection from desiccation, and so its evolution believed to have been prerequisite for terrestrial colonization. A major structural component apparently plant cuticles cutin, polyester hydroxy fatty acids; however, despite ubiquity, the details cutin polymeric structure mechanisms formation remodeling are not well understood. We recently reported polymerization in tomato ( S olanum...
The first committed step in triterpenoid biosynthesis is the cyclization of epoxysqualene into various triterpene alcohol isomers, a reaction catalyzed by oxidosqualene cyclases (OSCs). different OSCs have characteristic product specificities, which are mainly due to differences numbers high-energy intermediates enzymes can stabilize. goal this investigation was clone and characterize from tomato (Solanum lycopersicum), species known accumulate δ-amyrin its fruit cuticular wax, order gain...
The cuticle covers the aerial epidermis of land plants and plays a primary role in water regulation protection from external stresses. Remarkable species diversity structure composition its components, cutin wax, have been catalogued, but few functional or genetic correlations emerged. Tomato (Solanum lycopersicum) is part complex closely related wild endemic to northern Andes Galapagos Islands Sect. Lycopersicon). Although sharing an ancestor <7 million years ago, these are found diverse...
Approximately 1% of plant proteins are predicted to be post-translationally modified with a glycosylphosphatidylinositol (GPI) anchor that tethers the polypeptide outer leaflet plasma membrane. Whereas synthesis and structure GPI anchors is largely conserved across eukaryotes, repertoire functional domains present in GPI-anchored proteome has diverged substantially. In plants, this includes large fraction being further plant-specific arabinogalactan (AG) O-glycans. The importance development...
Softening is a hallmark of ripening in fleshy fruits, and has both desirable undesirable implications for texture postharvest stability. Accordingly, the timing extent pre-harvest associated textural changes following harvest are key targets improving fruit quality through breeding. Previously, we identified large effect locus with date firmness apple ( Malus domestica ) using genome-wide association studies (GWAS). Here, present additional evidence that polymorphisms or around transcription...
The aerial organs of plants are covered by the cuticle, a polyester matrix cutin and organic solvent-soluble waxes that is contiguous with polysaccharide cell wall epidermis. cuticle an important surface barrier between plant its environment, providing protection against desiccation, disease, pests. However, many aspects mechanisms biosynthesis, assembly, restructuring entirely unknown. To identify candidate proteins role in biogenesis, protein extract was obtained from tomato (Solanum...
The first committed step in triterpenoid biosynthesis is the cyclization of oxidosqualene to polycyclic alcohols or ketones C30H50O. It catalyzed by single cyclase (OSC) enzymes that can carry out varying numbers carbocation rearrangements and, thus, generate triterpenoids with diverse carbon skeletons. OSCs from plant species have been cloned and characterized, large majority them catalyzing relatively few rearrangement steps. was recently predicted special must exist form friedelin,...
Abstract Oat (Avena sativa L.) has a high concentration of oils, comprised primarily healthful unsaturated oleic and linoleic fatty acids. To accelerate oat plant breeding efforts, we sought to identify loci associated with variation in acid composition, defined as the types quantities We genotyped panel 500 cultivars genotyping-by-sequencing measured concentrations ten acids these grown two environments. Measurements individual were highly correlated across samples, consistent participating...
In order to understand factors controlling the synthesis and deposition of cellulose, we have studied Arabidopsis (Arabidopsis thaliana) double mutant shaven3 shaven3-like1 (shv3svl1), which was shown previously exhibit a marked cellulose deficiency. We discovered that exogenous sucrose (Suc) in growth medium greatly enhances reduction hypocotyl elongation content shv3svl1. This effect specific Suc not observed with other sugars or osmoticum. Live-cell imaging fluorescently labeled synthase...
Cell adhesion in plants is mediated predominantly by pectins, a group of complex cell wall associated polysaccharides. An Arabidopsis mutant, friable1 (frb1), was identified through screen T-DNA insertion lines that exhibited defective adhesion. Interestingly, the frb1 displayed both and organ dissociations also ectopic defects separation. The FRB1 gene encodes Golgi-localized, plant specific protein with only weak sequence similarities to known proteins (DUF246). Unlike other deficient...
Integration of multi-omics data improved prediction accuracies oat agronomic and seed nutritional traits in multi-environment trials distantly related populations addition to the single-environment prediction. Multi-omics has been shown be superior genomic with genome-wide DNA-based genetic markers (G) for predicting phenotypes. However, most existing studies were based on historical datasets from one environment; therefore, they unable evaluate efficiency populations. To fill those gaps, we...
Oat ranks sixth in world cereal production and has a higher content of health-promoting compounds compared with other cereals. However, there is neither robust oat reference genome nor transcriptome. Using deeply sequenced full-length mRNA libraries cultivar Ogle-C, de novo high-quality comprehensive seed transcriptome was assembled. With this QuantSeq 3' sequencing, gene expression quantified during development from 22 diverse lines across six time points. Transcript showed correlations...
The observable phenotype is the manifestation of information that passed along different organization levels (transcriptional, translational, and metabolic) a biological system. widespread use various omic technologies (RNA-sequencing, metabolomics, etc.) has provided plant genetics breeders with wealth on pertinent intermediate molecular processes may help explain variation in conventional traits such as yield, seed quality, fitness, among others. A major challenge effectively using these...