A5087415977- Aluminum toxicity and tolerance in plants and animals
- Plant Micronutrient Interactions and Effects
- Plant Stress Responses and Tolerance
- Genetic Mapping and Diversity in Plants and Animals
- Silicon Effects in Agriculture
- Genetics and Plant Breeding
- Phytase and its Applications
- Genetic and phenotypic traits in livestock
- Plant nutrient uptake and metabolism
- Bioinformatics and Genomic Networks
- Iron Metabolism and Disorders
- Plant Molecular Biology Research
- Genetically Modified Organisms Research
- Plant Genetic and Mutation Studies
- Gene expression and cancer classification
- Crop Yield and Soil Fertility
- Food composition and properties
- Wheat and Barley Genetics and Pathology
- Lipid metabolism and biosynthesis
- Seed and Plant Biochemistry
- Plant biochemistry and biosynthesis
- Plant Disease Resistance and Genetics
- Potato Plant Research
- Plant Pathogens and Resistance
- Metabolomics and Mass Spectrometry Studies
Ithaca College
2014-2024
Agricultural Research Service
2003-2017
United States Department of Agriculture
2003-2017
University of Illinois Urbana-Champaign
2017
Cornell University
2004-2015
Robert W. Holley Center for Agriculture & Health
2010-2015
Gifu University
2007
Okayama University
2006
Iowa State University
2003
Seoul Women's University
2003
Aluminum (Al) tolerance in Arabidopsis is a genetically complex trait, yet it mediated by single physiological mechanism based on Al-activated root malate efflux. We investigated possible molecular determinant for Al involving homolog of the wheat transporter, ALMT1 . This gene, named AtALMT1 (At1g08430), was best candidate from 14-member AtALMT family to be involved with expression patterns and genomic location. Physiological analysis transferred DNA knockout mutant as well...
Abstract Malate transporters play a critical role in aluminum (Al) tolerance responses for some plant species, such as Arabidopsis (Arabidopsis thaliana). Here, we further characterize AtALMT1, an aluminum-activated malate transporter, to clarify its specific release and Al stress responses. excretion from the roots of accession Columbia was sharply induced by Al, which is concomitant with induction AtALMT1 gene expression. The among rhizotoxic stressors, namely cadmium, copper, erbium,...
Background Advances in "omics" technologies have revolutionized the collection of biological data. A matching revolution our understanding systems, however, will only be realized when similar advances are made informatic analysis resulting "big data." Here, we compare capabilities three conventional and novel statistical approaches to summarize decipher tomato metabolome. Methodology Principal component (PCA), batch learning self-organizing maps (BL-SOM) weighted gene co-expression network...
Genome-wide association studies (GWAS) have identified loci linked to hundreds of traits in many different species. Yet, because linkage equilibrium implicates a broad region surrounding each locus, the causal genes often remain unknown. This problem is especially pronounced nonhuman, nonmodel species, where functional annotations are sparse and there frequently little information available for prioritizing candidate genes. We developed computational approach, Camoco, that integrates by GWAS...
Abstract Aluminum (Al) toxicity, which is caused by the solubilization of Al3+ in acid soils resulting inhibition root growth and nutrient/water acquisition, a serious limitation to crop production, because up one-half world's potentially arable land acidic. To date, however, no Al tolerance genes have yet been cloned. The physiological mechanisms are somewhat better understood; major documented mechanism involves Al-activated release Al-binding organic acids from tip, preventing uptake into...
Background Aluminum (Al) toxicity is a major worldwide constraint to crop productivity on acidic soils. Al becomes soluble at low pH, inhibiting root growth and severely reducing yields. Maize an important staple food commodity in soil regions, especially South America Africa where these soils are very common. exclusion intracellular tolerance have been suggested as two mechanisms for maize, but little known about the underlying genetics. Methodology An association panel of 282 diverse maize...
Background Maize is a major cereal crop widely consumed in developing countries, which have high prevalence of iron (Fe) deficiency anemia. The cause Fe these countries inadequate intake bioavailable Fe, where poverty factor. Therefore, biofortification maize by increasing concentration and or bioavailability has great potential to alleviate this deficiency. also model system for genomic research thus allows the opportunity gene discovery. Here we describe an integrated genetic physiological...
Controlling elemental composition is critical for plant growth and development as well the nutrition of humans who utilize plants food. Uncovering genetic architecture underlying mineral ion homeostasis in a first step towards understanding biochemical networks that regulate plant's (ionome). Natural accessions Arabidopsis thaliana provide rich source diversity leads to phenotypic differences. We analyzed concentrations 17 different elements 12 A. three recombinant inbred line (RIL)...
The ionome, or elemental profile, of a maize kernel can be viewed in at least two distinct ways. First, the collection elements within are food and feed for people animals. Second, ionome represents developmental end point that summarize life history plant, combining genetic programs environmental interactions. We assert single-kernel-based phenotyping is an effective method analysis, as it reasonable compromise between precision, efficiency, power. Here, we evaluate potential pitfalls this...
Dietary mineral deficiencies affect nearly half of the people on our planet, largely due to poverty. Enhancing nutritional quality—or biofortification—represents an efficient and sustainable potential solution this massive public health problem. To create biofortified crops, one must understand genetic environmental factors that influence ionome, or collection nutrients, in target organism tissue. We describe use quantitative trait loci (QTL) mapping characterize maize ( Zea mays L.) grain...
Abstract Oat (Avena sativa L.) has a high concentration of oils, comprised primarily healthful unsaturated oleic and linoleic fatty acids. To accelerate oat plant breeding efforts, we sought to identify loci associated with variation in acid composition, defined as the types quantities We genotyped panel 500 cultivars genotyping-by-sequencing measured concentrations ten acids these grown two environments. Measurements individual were highly correlated across samples, consistent participating...
Abstract Developmental responses associated with end-of-day far-red light (EOD-FR) signaling were investigated in maize (Zea mays subspecies mays) seedlings. A survey of genetically diverse inbreds temperate and tropical/semitropical origins, together teosinte parviglumis) a modern hybrid, revealed distinct elongation responses. mesocotyl response to the EOD-FR treatment was largely absent lines, but both hybrid inbred same magnitude as teosinte, suggesting that EOD-FR-mediated not lost...
Iron (Fe) deficiency is the most common micronutrient worldwide. biofortification a preventative strategy that alleviates Fe by improving amount of absorbable in crops. In present study, we used an vitro digestion/Caco 2 cell culture model as guiding tool for breeding and development two maize (Zea mays L.) lines with contrasting bioavailability (ie. Low High). Our objective was to confirm validate results approach. Also, compare capacities our hybrid varieties deliver hemoglobin (Hb)...
Plants have evolved a series of tolerance mechanisms to saline stress, which perturbs physiological processes throughout the plant. To identify genetic associated with salinity tolerance, we performed linkage analysis and genome-wide association study (GWAS) on maintenance root growth Arabidopsis thaliana in hydroponic culture weak severe NaCl toxicity. The top 200 single-nucleotide polymorphisms (SNPs) determined by GWAS could cumulatively explain approximately 70% variation observed at...
Abstract Plants obtain soil-resident elements that support growth and metabolism from the water-flow facilitated by transpiration active transport processes. The availability of in environment interacts with genetic capacity organisms to modulate element uptake through plastic adaptive responses, such as homeostasis. These interactions should cause elemental contents plants vary effects polymorphisms will be dramatically dependent on which plant is grown. To investigate genotype underlying...
Integration of multi-omics data improved prediction accuracies oat agronomic and seed nutritional traits in multi-environment trials distantly related populations addition to the single-environment prediction. Multi-omics has been shown be superior genomic with genome-wide DNA-based genetic markers (G) for predicting phenotypes. However, most existing studies were based on historical datasets from one environment; therefore, they unable evaluate efficiency populations. To fill those gaps, we...
Oat ranks sixth in world cereal production and has a higher content of health-promoting compounds compared with other cereals. However, there is neither robust oat reference genome nor transcriptome. Using deeply sequenced full-length mRNA libraries cultivar Ogle-C, de novo high-quality comprehensive seed transcriptome was assembled. With this QuantSeq 3' sequencing, gene expression quantified during development from 22 diverse lines across six time points. Transcript showed correlations...
Summary A quantitative in situ hybridization technique (quantitative whole‐mount hybridization, QISH) for plants is described. It employs direct of fluorescently labelled gene‐specific oligonucleotides large tissue pieces combined with optical sectioning. dramatically increases the throughput compared conventional antibody‐ and microtome‐based mRNA methods, while simultaneously eliminating artefact‐prone preparation steps that prevent reliable quantification methods. The key feature this...