A5103213891- Genetic Mapping and Diversity in Plants and Animals
- Chromosomal and Genetic Variations
- Plant Reproductive Biology
- Plant Surface Properties and Treatments
- Genomics and Phylogenetic Studies
- HER2/EGFR in Cancer Research
- Click Chemistry and Applications
- Estrogen and related hormone effects
- Postharvest Quality and Shelf Life Management
- Plant nutrient uptake and metabolism
- Plant Virus Research Studies
- Gastrointestinal Bleeding Diagnosis and Treatment
- Food composition and properties
- Nutrition, Genetics, and Disease
- Plant Molecular Biology Research
- Biofuel production and bioconversion
- Genomic variations and chromosomal abnormalities
- Plant Disease Resistance and Genetics
Air Force Medical University
2024
Xijing Hospital
2024
Rutgers, The State University of New Jersey
2016-2020
Northeast Agricultural University
2014
Grain starch and protein are synthesized during endosperm development, prompting the question of what regulatory mechanism underlies synchronization accumulation secondary primary gene products. We found that two endosperm-specific NAC transcription factors, ZmNAC128 ZmNAC130, have such a function. Knockdown expression ZmNAC130 with RNA interference (RNAi) caused shrunken kernel phenotype significant reduction protein. could show regulate Bt2 then reduce its level, rate-limiting step in...
Abstract Mutation of o2 doubles maize endosperm lysine content, but it causes an inferior kernel phenotype. Developing quality protein (QPM) by introgressing modifier s ( Mo2 s) into the mutant benefits millions people in developing countries where is a primary source. Here, we report genome sequence and annotation South African QPM line K0326Y, which assembled from single-molecule, real-time shotgun sequencing reads collinear with optical map. We achieve N50 contig length 7.7 million bases...
The chicken (Gallus gallus) is an important model organism that bridges the evolutionary gap between mammals and other vertebrates. Copy number variations (CNVs) are a form of genomic structural variation widely distributed in genome. CNV analysis has recently gained greater attention momentum, as identification CNVs can contribute to better understanding traits both humans animals. To detect CNVs, we genotyped 475 animals derived from two broiler lines divergently selected for abdominal fat...
Haplotype variation not only involves SNPs but also insertions and deletions, in particular gene copy number variations. However, comparisons of individual genomes have been difficult because traditional sequencing methods give too short reads to unambiguously reconstruct chromosomal regions containing repetitive DNA sequences. An example such a case is the protein family maize that acts as sink for reduced nitrogen seed. Previously, 41-48 copies alpha zein spread over six loci spanning...
All aerial epidermal cells in land plants are covered by the cuticle, an extracellular hydrophobic layer that provides protection against abiotic and biotic stresses prevents organ fusion during development. Genetic morphological analysis of classic maize adherent1 (ad1) mutant was combined with genome-wide binding MYB transcription factor FUSED LEAVES1 (FDL1), coupled transcriptional profiling fdl1 mutants. We show AD1 encodes epidermally-expressed 3-KETOACYL-CoA SYNTHASE (KCS) belonging to...
Abstract Serine functions as both a substrate for protein biosynthesis and signaling molecule growth development. However, the mechanism remains poorly understood. Here, we cloned functionally characterized maize (Zea mays) gene Dek20, which encodes phosphoglycerate dehydrogenase1 (PGDH1), rate-limiting enzyme in phosphorylated pathway of serine (PPSB). The dek20(Ser282Leu) mutation disrupts interaction between residues Ser282 His284, leading to release subsequently binds NAD+/NADH inhibit...
Summary Bulked segregant analysis ( BSA ) is used to identify existing or induced variants that are linked phenotypes. Although it widely in Arabidopsis and rice, remains challenging for crops with large genomes, such as maize. Moreover, of huge data sets can present a bottleneck linking phenotypes their molecular basis, especially geneticists without programming experience. Here, we identified two genes maize defective kernel mutants newly developed pipelines require no programing skills...
ABSTRACT In land plants all aerial epidermal cells are covered by the cuticle, an extracellular hydrophobic layer. The cuticle represents a primary barrier between and external environment, provides protection against abiotic biotic stresses, prevents organ fusion during development. Here we report cloning characterization of classic mutant maize called adherent1 ( ad1 ), first described century ago, show that AD1 encodes 3-KETOACYL-CoA SYNTHASE involved in deposition cuticular wax on...