A5067093821- Diabetes, Cardiovascular Risks, and Lipoproteins
- Cholesterol and Lipid Metabolism
- Lipoproteins and Cardiovascular Health
- Cancer, Lipids, and Metabolism
- Lipid metabolism and disorders
- Peroxisome Proliferator-Activated Receptors
- Liver Disease Diagnosis and Treatment
- Diet and metabolism studies
- Diet, Metabolism, and Disease
- Adipose Tissue and Metabolism
- Cardiovascular Function and Risk Factors
- Drug Transport and Resistance Mechanisms
- Pancreatic function and diabetes
- Diabetes Management and Research
- Cardiovascular Disease and Adiposity
- Diabetes Treatment and Management
- Diabetes and associated disorders
- Sirtuins and Resveratrol in Medicine
- Lipid metabolism and biosynthesis
- Paraoxonase enzyme and polymorphisms
- Sphingolipid Metabolism and Signaling
- Gut microbiota and health
- Caveolin-1 and cellular processes
- Folate and B Vitamins Research
- Pancreatitis Pathology and Treatment
Hospital de Sant Pau
2016-2025
Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas
2016-2025
Instituto de Salud Carlos III
2020-2025
Institut de Recerca Sant Pau
1999-2024
Institut Universitari d'Investigació en Atenció Primària Jordi Gol
2024
Universitat Autònoma de Barcelona
2014-2023
Institut d'Investigacions Biomèdiques de Barcelona
2012-2023
Cardiff University
2022
Park Plaza Hospital
2022
Institut d'Investigació Biomédica de Bellvitge
2017
Abstract Heart failure (HF) is increasing at an alarming rate, primary due to the rising in aging, obesity and diabetes. Notably, individuals with type 1 diabetes (T1D) face a significantly elevated risk of HF, leading more hospitalizations increased case fatality rates. Several factors contribute HF T1D, including poor glycemic control, female gender, smoking, hypertension, BMI, albuminuria. However, early intensive control can mitigate long-term T1D. The pathophysiology diabetes-associated...
Background: Increased epicardial adipose tissue (EAT) volume is a common feature in type 2 diabetes (T2DM) which directly associated with heart failure and advanced atherosclerosis. We aimed to evaluate lipoprotein-related biomarkers of EAT T2DM patients before after glycemic control. Methods: This study included 36 optimization control on 14 healthy controls (HCs). was measured using computed tomography imaging indexed the body surface area (iEAT). Biochemical lipid profiles were determined...
Our understanding of apolipoprotein A-II (apoA-II) physiology is much more limited than that apoA-I. However, important and rather surprising advances have been produced, mainly through analysis genetically modified mice. These results reveal a positive association apoA-II with FFA VLDL triglyceride plasma concentrations; however, whether this due to increased synthesis or decreased catabolism remains matter controversy. As apoA-II-deficient mice present phenotype insulin hypersensitivity,...
Apolipoprotein A-II (apoA-II), the second major high-density lipoprotein (HDL) apolipoprotein, has been linked to familial combined hyperlipidemia. Human apoA-II transgenic mice constitute an animal model for this proatherogenic disease. We studied ability of human HDL protect against oxidative modification apoB-containing lipoproteins. When challenged with atherogenic diet, antigens related low-density (LDL) oxidation were markedly increased in aorta 11.1 (high expressor). from control and...
Deletion of glycine N-methyltransferase (GNMT), the main gene involved in liver S-adenosylmethionine (SAM) catabolism, leads to hepatic accumulation this molecule and development fatty fibrosis mice. To demonstrate that excess SAM is agent contributing disease GNMT knockout (KO) mice, we treated 1.5-month-old GNMT-KO mice for 6 weeks with nicotinamide (NAM), a substrate enzyme NAM N-methyltransferase. administration markedly reduced content, prevented DNA hypermethylation, normalized...
The HDL (high-density lipoprotein)-mediated stimulation of cellular cholesterol efflux initiates macrophage-specific reverse transport (m-RCT), which ends in the fecal excretion macrophage-derived unesterified (UC). Early studies established that LDL (low-density lipoprotein) particles could act as efficient intermediate acceptors cellular-derived UC, thereby preventing saturation and facilitating their capacity. However, capacity to a plasma reservoir its potential impact supporting m-RCT...
A high-saturated fatty acid- and cholesterol-containing (HFHC) diet is considered to be a major risk factor for cardiovascular disease. The present study aimed determine the effects of this Western-type on high-density lipoprotein (HDL) metabolism reverse cholesterol transport (RCT) from macrophages feces.Experiments were carried out in mice fed low-fat, low-cholesterol diet, an HFHC or without added (high-saturated acid [HFLC]). caused significant increase plasma cholesterol, HDL liver...
Introduction— Apolipoprotein (apo) A-II is the second most abundant high-density lipoprotein (HDL) apolipoprotein. We assessed mechanism involved in altered postprandial triglyceride-rich metabolism of female human apoA-II-transgenic mice (hapoA-II-Tg mice), which results up to an 11-fold increase plasma triglyceride concentration. The relationships between apoA-II, HDL composition, and lipase (LPL) activity were also analyzed a group normolipidemic women. Methods Results— Triglyceride-rich...
Low density lipoprotein is a heterogeneous group of lipoproteins that differs in lipid and protein composition. One copy apolipoprotein (apo)B accounts for over 95% the LDL protein, but presence minor proteins could disturb its biological behavior. Our aim was to study content subfractions separated by anion exchange chromatography. Electropositive [LDL(+)] native form, whereas electronegative [LDL⁻] atherogenic fraction present blood. LC-ESI MS/MS analysis both fractions identified up 28...
Objective To investigate longitudinal changes in the liver stiffness measurement (LSM) general adult population without known disease and to describe its association with metabolic risk factors, a special focus on subjects non-alcoholic fatty (NAFLD) dysglycemia. Material Methods A population-based cohort study was conducted Catalonia. LSM measured by transient elastography (TE) at baseline follow-up (median: 4.2 years). Subgroup NAFLD dysglycemia were analyzed. Moderate-to-advanced fibrosis...
The prevalence of metabolic dysfunction-associated steatotic liver disease (MASLD), a leading cause chronic disease, has increased worldwide along with the epidemics obesity and related dysmetabolic conditions characterized by impaired glucose metabolism insulin signaling, such as type 2 diabetes mellitus (T2D). MASLD can be defined an excessive accumulation lipid droplets in hepatocytes that occurs when hepatic is totally surpassed. This inflexibility constitutes central node pathogenesis...
The low number of postnatal endothelial progenitor cells (EPC) in the circulation limits their therapeutic application cardiovascular medicine. Processed lipoaspirate (PLA) differentiate into osteoid, adipose, muscle, and cartilaginous cells. This study examines potential PLA as a source EPCs.PLA obtained from human lipoaspirates were cultured for 1 week serum-depleted medium to form three-dimensional cell clusters (3DCC). phenotype 3DCC-derived was assessed by immunofluorescence staining...
Liver X receptors (LXRs) exert key functions in lipid homeostasis and control of inflammation. In this study we have explored the impact LXR activation on macrophage response to endogenous inflammatory cytokine IFN-γ. Transcriptional profiling studies demonstrate that ∼38% IFN-γ-induced transcriptional is repressed by macrophages. LXRs also mediated inhibitory effects selected genes primary microglia a model neuroinflammation vivo. resulted reduced STAT1 recruitment promoters tested without...
The concentration of high density lipoproteins (HDL) is inversely related to the risk atherosclerosis. two major protein components HDL are apolipoprotein (apo) A-I and apoA-II. To study role apoA-II in lipoprotein metabolism atherosclerosis, we have developed three lines C57BL/6 transgenic mice expressing human (lines 25.3, 21.5, 11.1). Northern blot experiments showed that mRNA was present only liver mice. SDS-polyacrylamide gel electrophoresis Western analysis demonstrated a 17.4-kDa...
We report on the effect of human apolipoprotein (apo) A-II transgene expression atherosclerosis susceptibility in two transgenic lines (25.3 and 11.1) whose plasma apoA-II concentrations (∼23 96 mg/dl, respectively) span normal range humans. After 9 months an atherogenic diet, 25.3 11.1 mice developed aortic atherosclerotic lesions that were ∼1.7- 7-fold, respectively, more extensive than those non-transgenic control mice. However, there was no difference area when fed a regular chow diet....