A5089072161- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Plant Disease Resistance and Genetics
- Protist diversity and phylogeny
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Insect symbiosis and bacterial influences
- Microbial Community Ecology and Physiology
- Evolution and Genetic Dynamics
- Toxoplasma gondii Research Studies
- Insect-Plant Interactions and Control
- Parasitic Infections and Diagnostics
- Legume Nitrogen Fixing Symbiosis
- Plant Taxonomy and Phylogenetics
- Bat Biology and Ecology Studies
- T-cell and B-cell Immunology
- Amoebic Infections and Treatments
- Study of Mite Species
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Diatoms and Algae Research
- Insect and Arachnid Ecology and Behavior
- Invertebrate Immune Response Mechanisms
- Mosquito-borne diseases and control
The University of Texas at Arlington
2005-2023
University of Utah
2014-2021
Cornell University
2020
University of Georgia
2005-2007
Science Oxford
2005
Universidad de Salamanca
2005
Pasadena City College
2005
Bethesda University
2005
Seattle University
2005
United States Department of Justice
2005
We describe the draft genome of microcrustacean Daphnia pulex, which is only 200 megabases and contains at least 30,907 genes. The high gene count a consequence an elevated rate duplication resulting in tandem clusters. More than third Daphnia's genes have no detectable homologs any other available proteome, most amplified families are specific to lineage. coexpansion interacting within metabolic pathways suggests that maintenance duplicated not random, analysis expression under different...
Culex quinquefasciatus (the southern house mosquito) is an important mosquito vector of viruses such as West Nile virus and St. Louis encephalitis virus, well nematodes that cause lymphatic filariasis. C. one species within the pipiens complex can be found throughout tropical temperate climates world. The ability to take blood meals from birds, livestock, humans contributes its pathogens between species. Here, we describe genomic sequence quinquefasciatus: Its repertoire 18,883...
Cryptophyte and chlorarachniophyte algae are transitional forms in the widespread secondary endosymbiotic acquisition of photosynthesis by engulfment eukaryotic algae. Unlike most plastid-bearing algae, miniaturized versions endosymbiont nuclei (nucleomorphs) persist cryptophytes chlorarachniophytes. To determine why, to address other fundamental questions about eukaryote-eukaryote endosymbiosis, we sequenced nuclear genomes cryptophyte Guillardia theta Bigelowiella natans. Both have >21,000...
A recipe for new genes Most lineages contain evolutionarily novel genes, but their origin is not always clear. Cosby et al. investigated the of families lineage-specific vertebrate (see Perspective by Wacholder and Carvunis). Fusion between transposable elements (TEs) host gene exons, once incorporated into genome, could generate functional genes. Examination KARABINER , a bat that arose through this process, shows how retention part TE within allows transcribed protein to bind throughout...
Rolling-circle (RC) transposons, or Helitrons, are a newly recognized group of eukaryotic transposable elements abundant in the genomes plants, invertebrates, and zebrafish. We provide evidence for colonization mammalian genome by which has not been reported previously. identified characterized two families Helitrons little brown bat Myotis lucifugus. The consensus sequence first family, HeliBat1, displays hallmarks an autonomous Helitron, including coding capacity approximately 1,500-aa...
DNA transposons, or class 2 transposable elements, have successfully propagated in a wide variety of genomes. However, it is widely believed that transposon activity has ceased mammalian genomes for at least the last 40 million years. We recently reported evidence relatively recent hAT and Helitron two distinct groups lineage vespertilionid bat Myotis lucifugus . Here, we describe seven additional families also been active lineage. Early genome evolution was dominated by s, mariner -like Tc2...
The germline genome of the binucleated ciliate Tetrahymena thermophila undergoes programmed chromosome breakage and massive DNA elimination to generate somatic genome. Here, we present a complete sequence assembly analyze multiple features its structure relationship genome, shedding light on mechanisms rearrangement as well evolutionary history this remarkable germline/soma differentiation. Our results strengthen notion that complex, dynamic, ongoing interplay between mobile elements host...
Metaseiulus occidentalis is an eyeless phytoseiid predatory mite employed for the biological control of agricultural pests including spider mites. Despite appearances, these predator and prey mites are separated by some 400 Myr evolution radically different lifestyles. We present a 152-Mb draft assembly M. genome: Larger than that its favored prey, Tetranychus urticae, but considerably smaller those many other chelicerates, enabling extremely contiguous complete to be built-the best arachnid...
Abstract Helitron transposons capture and mobilize gene fragments in eukaryotes, but experimental evidence for their transposition is lacking the absence of an isolated active element. Here we reconstruct Helraiser , ancient element from bat genome, use this transposon as tool to unravel mechanism transposition. A hairpin close 3′-end functions a terminator. However, can be bypassed by transposase, resulting transduction flanking sequences new genomic locations. generates covalently closed...
Horizontal transfer (HT) of genes is known to be an important mechanism genetic innovation, especially in prokaryotes. The impact HT transposable elements (TEs), however, has only recently begun receive widespread attention and may significant due their mutagenic potential, inherent mobility, abundance. Helitrons, also as rolling-circle transposons, are a distinctive subclass TE with unique transposition mechanism. Here, we describe the first evidence for repeated four different families...
The largest component of plant and animal genomes characterized to date is transposable elements (TEs). availability a significant amount Lotus japonicus genome sequence has permitted for the first time comprehensive study TE landscape in legume species. Here we report results combined computer-assisted experimental analysis TEs 32.4 Mb finished TAC clones. While facilitated determination abundance diversity, complete sequences identification full-length TEs, which design tools genomewide...
Rolling-circle transposons (Helitrons) are a newly discovered group of mobile DNA widespread in plant and invertebrate genomes but limited to the bat family Vespertilionidae among mammals. Little is known about long-term impact Helitron activity because where has been extensively studied predominated by young families. Here, we report comprehensive catalog vetted Helitrons from 7× Myotis lucifugus genome assembly. To estimate timing transposition, scored presence/absence across related...
The tomato russet mite, Aculops lycopersici, is among the smallest animals on earth. It a worldwide pest and can potently suppress host’s natural resistance. We sequenced its genome, first of an eriophyoid, explored whether there are genomic features associated with mite’s minute size lifestyle. At only 32.5 Mb, genome yet reported for any arthropod and, reminiscent microbial eukaryotes, exceptionally streamlined. has few transposable elements, tiny intergenic regions, remarkably...
We report the first comprehensive analysis of transposable element content in compact genomes (∼20 Mb) four species Entamoeba unicellular protozoans for which draft sequences are now available. histolytica and dispar, two human parasites, have many retrotransposons, but few DNA transposons. In contrast, reptile parasite invadens free-living moshkovskii contain long interspersed elements harbor diverse recently amplified populations Representatives three transposase superfamilies...
Abstract Transposons of the Mutator (Mu) superfamily have been shown to play a critical role in evolution plant genomes. However, identification transposons other eukaryotes has quite limited. Here we describe previously uncharacterized group DNA designated Phantom identified genomes wide range eukaryotic taxa, including many animals, and provide evidence for its inclusion within superfamily. Interestingly three proteins were also two insect viruses phylogenetic analysis suggests horizontal...
The Drosophila INterspersed Elements-1 (DINE-1/INE1) transposable elements (TEs) are the most abundant component of melanogaster genome and have been associated with functional gene duplications. DINE-1 TEs do not encode any proteins (non-autonomous) thus moved by autonomous partners. identity partners has a mystery. They allied to Helitrons (rolling-circle transposons), MITEs (DNA non-LTR retrotransposons different authors.We report multiple lines bioinformatic evidence that illustrate...
We investigate the role of recombination in transposable element (TE) proliferation cyclical parthenogen, Daphnia pulex . Recombination provides a mechanism by which rate both TE gain and loss can be accelerated, duality that has long intrigued many biologists interested influence sex on mutation accumulation. compared loads among populations D. where occurs regularly (cyclical parthenogens or ‘sexuals’) with those ability to reproduce sexually been completely lost (obligate ‘asexuals’) for...