A5100766888- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Cancer-related molecular mechanisms research
- T-cell and B-cell Immunology
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Helicobacter pylori-related gastroenterology studies
- Ferroptosis and cancer prognosis
- Lung Cancer Treatments and Mutations
- Immune cells in cancer
- Psoriasis: Treatment and Pathogenesis
- Immunodeficiency and Autoimmune Disorders
- Immune Response and Inflammation
- Galectins and Cancer Biology
- Cancer, Hypoxia, and Metabolism
- Cell Adhesion Molecules Research
- CAR-T cell therapy research
- Lung Cancer Diagnosis and Treatment
- Epigenetics and DNA Methylation
- interferon and immune responses
- Biomarkers in Disease Mechanisms
- Estrogen and related hormone effects
- Inflammatory mediators and NSAID effects
- Lung Cancer Research Studies
Huazhong University of Science and Technology
2016-2025
Tongji Hospital
2016-2025
Guangxi Medical University
2021
Thoracic Surgery Foundation
2020
Ministry of Education of the People's Republic of China
2017
Tumor Hospital of Guangxi Medical University
2017
Beth Israel Deaconess Medical Center
2008-2016
Harvard University
2004-2016
Cancer Research Center
2012
Massachusetts General Hospital
2006
During activation, T cells undergo metabolic reprogramming, which imprints distinct functional fates. We determined that on PD-1 ligation, activated are unable to engage in glycolysis or amino acid metabolism but have an increased rate of fatty β-oxidation (FAO). promotes FAO endogenous lipids by increasing expression CPT1A, and inducing lipolysis as indicated elevation the lipase ATGL, marker glycerol release acids. Conversely, CTLA-4 inhibits without augmenting FAO, suggesting sustains...
Rational: LDCT screening can identify early-stage lung cancers yet introduces excessive false positives and it remains a great challenge to differentiate malignant tumors from benign solitary pulmonary nodules, which calls for better non-invasive diagnostic tools.Methods: We performed DNA methylation profiling by high throughput bisulfite sequencing in tissue samples (nodule size < 3 cm diameter) learn patterns that cancerous lesions.Then we filtered out exhibiting background circulating...
The outbreak of corona virus disease 2019 (Covid-19) imposes a major challenge in managing patients undergoing surgical operation. In this study, we analyzed clinical and transmission features 25 cases Covid-19 from single thoracic department, including 13 12 health care staff. There were males females. median age the was 61 (range: 51 to 69) years. staff 35 22 51) By end follow-up date (Mar. 3, 2020), there 16 non-severe (64%) 9 severe (36%), 5 dead (20%). Nineteen (76%) infected confirmed...
IFN‐γ plays a crucial role in anti‐tumor responses and also induces expression of PD‐L1, well‐established inhibitor immune function. Understanding how molecular signaling regulates the function might improve its efficacy. Here, we show that tumor alone has no significant prognostic value patients with locally advanced lung adenocarcinoma. Surprisingly, tumors expressing both PD‐L1 have best prognosis compared to those or alone. Transcriptome analysis demonstrated tissues display gene...
Interferon-gamma (IFN-γ) is a major effector molecule of immunity and common feature tumors responding to immunotherapy. Active IFN-γ signaling can directly trigger apoptosis cell cycle arrest in human cancer cells. However, the mechanisms underlying these actions remain unclear. Here, we report that rapidly increases protein synthesis causes unfolded response (UPR), as evidenced by increased expression glucose-regulated 78, activating transcription factor-4, c/EBP homologous (CHOP) cells...
Glutamine metabolism is emerging as a target for improving immunotherapy efficacy. However, the outcomes remain inconclusive. Given that tumor-intrinsic response to interferon-γ (IFN-γ) key determinant of efficacy, we investigated whether and how glutamine deprivation in cancer cells affects their IFN-γ. By using human lung cell lines, patient-derived tumor explants, syngeneic mouse model cancer, demonstrated reduced IFN-γ-driven by promoting autophagy-dependent IFN-γ receptor (IFNGR1)...
Recently, it has become clear that dendritic cells (DCs) are essential for the priming of T cell responses. However, their role in maintenance peripheral tolerance remains largely undefined. Herein, an antigen-presenting (APC) transfer system was devised and applied to experimental allergic encephalomyelitis (EAE), evaluate contribution DCs play tolerance. The CD8alpha(-)CD4(+) subset, a minor population among splenic DCs, found mediate both bystander suppression against diverse...
Abstract Regulatory T cells (Tregs) are committed to suppressive functions. Recently, it was proposed that Tregs could produce IL-17 under proinflammatory, polarizing conditions. We studied the role of on production in absence exogenous cytokines and insults. Using vitro vivo approaches, we determined neutral conditions, simultaneous activation naive CD4+ conventional presence APCs resulted conversion into IL-17–producing cells, endogenous IL-1β mandatory this process. Mechanistic analysis...
Stimulator of interferon genes (STING) pathway activation predicts the effectiveness targeting PD-1/PD-L1 axis in lung cancer. Active IFN-γ signaling is a common feature tumors that respond to blockade. The connection between and STING cancer cells has not been documented. We showed caused DNA damage accumulation cytosolic dsDNA, leading cGAS- IFI16-dependent adenocarcinoma cells. IFN-γ-induced iNOS expression nitric oxide production were responsible for activation. Additional etoposide...
Programmed death-ligand 1 (PD-L1) is thought to play a critical role in immune escape by cancer, but whether PD-L1 expression can influence prognosis of patients with solid tumors controversial. Therefore, we meta-analyzed available data on correlates overall survival (OS) such patients. PubMed, EMBASE and other databases were systematically searched for cohort or case-control studies examining the possible correlation between OS tumors. was compared positive negative using scatter plots,...
The repetitive antigen stimulation during chronic infection often leads to the accumulation of CD8+CD57+ T cells. These cells express high levels interferon-γ, granzyme B and perforin with elevated cytolytic effect, are considered as most potent for combating chronical viral infection. status in non-small cell lung cancer (NSCLC) has not been well defined. We used flow cytometry undertook a systemic approach examine frequency, immunophenotyping functional properties peripheral blood, tumor...
Abstract Background IL-15 plays a vital role in enhancing NK cell- and T-cell-mediated antitumor immune responses; however, the direct effect of on tumor cells has not been fully elucidated. Herein, we investigated lung adenocarcinoma cells. Methods Silencing overexpression techniques were used to modify endogenous expression Transwell assays assess cell migration invasion; live-cell analysis system was evaluate motility; cellular morphological changes quantified by confocal fluorescence...
Several immune-based approaches are being considered for modulation of inflammatory T cells and amelioration autoimmune diseases. The most recent strategies include simulation peripheral self-tolerance by injection adjuvant free antigen, local delivery cytokines genetically altered cells, interference with the function costimulatory molecules. Although promising results have been obtained from these studies that define mechanisms cell modulation, efficacy, practicality, toxicity, concerns...
CD4+Foxp3+ T regulatory cells (Tregs) display phenotypic and functional plasticity that is regulated by cytokines other immune cells. Previously, we determined during co-culture with CD4+CD25− antigen presenting cells, Tregs produced IL-17. Here, investigated the mechanisms underlying differentiation of IL-17-producing Treg (Tr17) their molecular properties. We stimulation via TCR/CD3 CD28, combination IL-1β IL-2 was necessary sufficient for generation Tr17 expressed Runx1 transcription...
Background/Aims: Increasing evidence suggests that T-UCRs are involved in the development of cancer. In this study, we evaluated role a macrophage-derived T-UCR, uc.306, prognosis hepatitis B (HBV)-related hepatocellular carcinoma (HCC). Methods: The uc.306 was obtained by screening microassay data during polarization U937 cells from M2 to M1 phenotype. Uc.306 and macrophage molecule markers were detected qPCR. Immunohistochemical (IHC) assays used examine M1/M2 status 90 paired HCC tissues....
The prognosis of ground glass opacity featured lung adenocarcinomas (GGO-LUAD) is significantly better than that solid nodule (SN-LUAD), but the specific reasons behind their indolent tumor behavior are still unclear. purpose this study to investigate differences in intratumoral microvessels, related angiogenic factors and important stromal cells. Thirty patients (15 paired only with GGO or SN) diagnosed pathological stage 0-I adenocarcinoma who underwent surgical treatment were included...
Background A subset of CD4 + T cells with cytotoxic activity has been identified, and these exert their effects by expressing perforin granzymes. Despite the progress made in characterizing various diseases, status non-small cell lung cancer (NSCLC) underlying mechanisms involved promoting intratumoral T-cell activation remain unclear. Methods We used flow cytometry to examine phenotypic functional properties GzmB peripheral blood tumor tissues patients NSCLC. Loss-of-function analyses RNA...
The prognosis of ground glass opacity featured lung adenocarcinomas (GGO-LUAD) is significantly better than that solid nodule (SN-LUAD), but the underlying reasons remain unclear. Ki-67 and cell cycle regulator proteins are highly expressed in many cancers linked to prognosis. This study aims investigate their differential expression LUAD with different radiological subtypes. Patients resected pathological stage 0-III our department between July 2019 March 2022 were retrospectively reviewed....
Tumor angiogenesis is a critical biological hallmark of cancer, which involves multiple molecularly regulated signaling pathways, including the angiopoietin (ANGPT)-Tie2 and vascular endothelial growth factor (VEGF) pathways. Despite initial optimism, targeting tumor in treatment lung adenocarcinoma (LUAD) has been unsatisfactory. Currently, monotherapy with PD-1/PD-L1 inhibitors, or their combination bevacizumab, considered standard therapeutic approach for LUAD. Recent studies have shown...
IFNγ-mediated signaling in tumor cells can induce immunosuppressive responses and cause resistance to immunotherapy. Blocking TGFβ promotes T lymphocyte infiltration turns immunologically cold tumors into hot tumors, thereby improving the efficacy of Several studies have shown that inhibits IFNγ immune cells. We thus sought determine whether affects plays a role development acquired stimulation increased SHP1 phosphatase activity an AKT-Smad3-dependent manner, decreased tyrosine...
Abstract Lymph nodes (LNs) are the staging grounds for anti-tumor immunity, therefore their high susceptibility to metastatic colonization is a paradox. Previous studies have suggested that extrinsic tumor-derived factors precondition draining LN enable tumor cell survival by promoting state of immune suppression. Here, we investigate whether properties itself may impede its ability clear metastasizing cells. Using multiple immunocompetent transplant models, show LNs possess intrinsic...