The presence of nerve growth factor (NGF) mRNA and protein in the rat central nervous system is documented. Blot-hybridization analysis showed an abundance NGF hippocampus, cerebral cortex, olfactory bulb. Enzyme immunoassay confirmed significant levels a NGF-like hippocampus cortex. Bioassay immunoaffinity-purified from these regions was physiologically indistinguishable NGF. Immunohistochemistry revealed widespread distribution reactivity adult brain, preferentially fiber tracts....

Abstract The neurotrophin gene family includes four structurally related proteins with neurotrophic activities. Two of them, nerve growth factor and brain‐derived (BDNF), have been studied in detail information has recently emerged on the expression function third member, neurotrophin‐3. In contrast, little is available neurotrophin‐4 (NT‐4), most isolated member this family. report we used a sensitive RNAase protection assay to analyse developmental NT‐4 mRNA rat brain 12 different...

A rat cell line (A2T2C4) transformed with adenovirus type 2 elicited cytotoxic T lymphocytes in syngeneic rats. Cytotoxicity was abolished by a rabbit antiserum directed against the major histocompatibility (AgB) antigens and raised virus-transformed line. The immunoprecipitated surface proteins apparent molecular weights of 45,000, 19,000, 17,000, 12,000 from A2T2C4 cells but it displayed no reactivity primary fibroblasts spleen cells. AgB precipitated 19,000-dalton component which not...

Northern blot analysis was used to demonstrate high levels of hippocampus-derived neurotrophic factor/neurotrophin-3 (HDNF/NT-3) mRNA in the embryonic day (E) 13 - 14 and 15 16 spinal cord. The level decreased at E18 19 remained same until postnatal (P) 1, after which it further a below detection limit adult. In situ hybridization revealed that NT-3 detected developing cord derived from motoneurons decrease seen caused by reduction number expressing mRNA. distribution mRNA-expressing cells...

Auto-ignition with SI-compression ratio can be achieved by replacing some of the fresh charge hot residuals. In this work an engine is run a negative valve overlap (NVO) trapping By increasing NVO, thus raising initial temperature it possible to investigate intermediate zone between SI and HCCI as amount residuals increased. Recent research has shown potential using spark assistance aid gasoline combustion at operating conditions, even extend regime into regions where unsupported impossible....

Early region 3 of the adenovirus type 2 genome encodes three proteins with molecular weights 16,000, 14,500, and 14,000 (E2/16, E3/14.5, E3/14). The E3/16 protein is precursor to E3/19 glycoprotein around 1500 daltons larger than unglycosylated E3/19O protein. E3/14.5 E3/14 are structurally related each other but different from E3/16. Three mRNA species were identified for E3/16; all have common 5' ends same spliced 3' ends. was translated a 13S structure as followed by second end. A partial...

A flow visualization study of instabilities caused by Coriolis effects in plane rotating Poiseuille has been carried out. The primary instability takes the form regularly spaced roll cells aligned direction. They may occur at Reynolds numbers as low 100, i.e. almost two orders magnitude lower than critical number for Tollmien-Schlichting waves channel without rotation. development such was studied a function both and rotation rate their properties compared with results from linear spatial...

Complementary strand-specific adenovirus DNA of full length or from endonuclease BamHI fragments was used as a probe to estimate the fractional representation and abundance viral sequences in five hamster cell lines (Ad2HE1-5) transformed with UV-inactivated type 2. The fraction genome present varied 44% Ad2HE5 line 84% Ad2HE3 line. number copies per diploid equivalent ranged 1.8 Ad2HE1 7.1 Ad2HE4 In vivo labeling [35S]methionine followed by immunoprecipitation an antiserum against 2 early...

The gene encoding the human vasoactive intestinal peptide (VIP) and histidine-methionine amide (PHM-27) hormone was isolated from lambda phage libraries. found to be composed of seven exons spanning approximately 9 kilobase pairs. first exon codes for an untranslated leader sequence, second a putative signal peptide. DNA sequences coding VIP PHM-27 hormones are located in two different exons. Southern blot analysis with genomic suggested that single copy VIP/PHM-27 is present haploid genome....

Summary. Fasting is generally accompanied by a decrease in energy metabolism and hormones. On the other hand, indirect evidence has suggested that response to adrenergic agonists may be maintained or even increased malnutrition. The present study evaluated whether weight‐losing patients with without cancer have plasma concentrations of catecholamines different responses intravenously infused adrenaline compared weight‐stable individuals. Eight malnourished 10 non‐cancer (11% weight loss)...

An adenovirus type 5 host range mutant (hr-1) located in region E1A and phenotypically defective expressing viral messenger ribonucleic acid (RNA) from other early regions (Berk et al., Cell 17:935-944, 1979) was analyzed for accumulation of RNA the presence protein synthesis inhibitors. Nuclear transcribed all at same rate, regardless whether drug present or absent. As expected, low undetectable levels were found cytoplasm hr-1-infected cells compared with wild-type absence drug. When...

A 15,000-dalton protein was purified from HeLa cells infected with adenovirus type 2. Proteins solubilized a membrane fraction of lytically used as the starting material for purification. Subsequent purification steps involved lentil-lectin, phosphocellulose, hydroxyapatite, DEAE-cellulose, and aminohexyl-Sepharose chromatographies. monospecific antiserum, raised against protein, immunoprecipitated encoded in early-region E1B (E1B/15K protein) 2 DNA. Tryptic finger print analysis revealed...

We present a 1,040-base-pair-long sequences of adenoviruses type 2 DNA which encodes the complete gene for precursor polypeptide VI (pVI). pVI consists 250 amino acids amounting to molecular weight 26,990. The proteolytic cleavage maturing virion removes 33 from amino-terminal end polypeptide, thus giving mature 23,400. UAA stop codon terminating translation is separated by 84 nucleotides initiator triplet hexon gene. Both polypeptides are encoded same translational reading frame, suggesting...

An adenovirus type 2 early glycoprotein with an apparent molecular weight of 19,000 (E19K) in sodium dodecyl sulfate-polyacrylamide gels has been extensively purified. Purification involved detergent solubilization membrane fractions from infected cells, followed by affinity chromatography on a lectin column and DEAE-Sephadex chromatography. The purified material contained three polypeptides (E40K, E19K, E17.5K), approximately 90% the E19K moiety. All yielded identical tryptic peptide maps....

Heat transfer losses are one of the largest loss contributions in a modern internal combustion engine. The aim this study is to evaluate contribution piston bowl type and swirl ratio heat performance. A commercial CFD tool used carry out simulations four different geometries, at three engine loads with two ratios each load point. One geometries as reference point, where results validated test data. All other scaled same compression make use fuel injection, variation spray target between...