A5100777754- Acute Myeloid Leukemia Research
- Retinoids in leukemia and cellular processes
- Cholesterol and Lipid Metabolism
- Cell death mechanisms and regulation
- Lipid metabolism and biosynthesis
- Hematopoietic Stem Cell Transplantation
- Lymphoma Diagnosis and Treatment
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Genomics, phytochemicals, and oxidative stress
- Endoplasmic Reticulum Stress and Disease
- Antioxidant Activity and Oxidative Stress
- Chronic Lymphocytic Leukemia Research
- Cancer, Lipids, and Metabolism
- Aldose Reductase and Taurine
- Hematological disorders and diagnostics
- Heat shock proteins research
- Plant biochemistry and biosynthesis
- Acute Lymphoblastic Leukemia research
- Hepatitis C virus research
- Chemotherapy-induced organ toxicity mitigation
- Urticaria and Related Conditions
- Sphingolipid Metabolism and Signaling
- Acute Kidney Injury Research
- Gastric Cancer Management and Outcomes
- Forensic Toxicology and Drug Analysis
Universidad de Salamanca
2008-2024
Centro de Investigación del Cáncer
2008-2024
Instituto de Investigación Biomédica de Salamanca
2012-2024
Centro de Investigación Biomédica en Red de Cáncer
2019-2024
Spanish Multiple Sclerosis Network
2021
Hospital Universitari Arnau de Vilanova
2017
Hospital Universitario Ramón y Cajal
2002-2014
Instituto de Biologia Molecular e Celular
2008-2013
Hospital Clínico Universitario de Caracas
2013
Consejo Superior de Investigaciones Científicas
2001-2010
Multiple myeloma remains largely incurable. However, a few patients experience more than 10 years of relapse-free survival and can be considered as operationally cured. Interestingly, long-term disease control in multiple is not restricted to with complete response, since some revert having profile monoclonal gammopathy undetermined significance. We compared the distribution compartments lymphocytes dendritic cells bone marrow peripheral blood (n=28), newly diagnosed significance (n=23),...
Abstract A heterogeneous spectrum of immunophenotypic abnormalities have been reported in myelodysplastic syndromes (MDS). However, most studies are restricted to the analysis CD34 + cells and/or other major subsets − cells, frequently not exploring diagnostic and prognostic impact immunophenotyping. Methods: We propose for first time an score (IS) based on altered distribution features maturing/mature compartments bone marrow (BM) hematopoietic 56 patients with MDS that could contribute a...
Cholesterol is a major lipid component of the plasma membrane in animal cells. In addition to its structural requirement, cholesterol essential cell proliferation and other processes. The aim present study was elucidate stringency requirement for as regulator cycle progression, compared with sterols biosynthesis pathway. Human promyelocytic HL-60 cells were cultured cholesterol-free medium treated different distal inhibitors (zaragozic acid, SKF 104976, SR 31747, BM 15766, AY 9944), which...
The observation that genistein may behave as a pro-oxidant agent lead us to examine the capacity of this isoflavone modulate toxicity oxidation-sensitive anti-leukemic arsenic trioxide (ATO), and for comparison other anti-tumor drugs. Co-treatment with increased ATO-provoked apoptosis activated regulatory events (Bcl-X(L) down-regulation, cytochrome c Omi/HtrA2 release from mitochondria, XIAP decrease caspase-8/Bid caspase-3 activation) in U937 promonocytes human leukemia cell lines (HL60,...
Multiparameter flow cytometry has become an essential tool for monitoring response to therapy in hematological malignancies, including B-cell chronic lymphoproliferative disorders (B-CLPD). However, depending on the expertise of operator minimal residual disease (MRD) can be misidentified, given that data analysis is based definition expert-based bidimensional plots, where selects subpopulations interest. Here, we propose and evaluate a probabilistic approach pattern classification tools...
Dietary phytosterols are cholesterol-lowering agents that interfere with the intestinal absorption of cholesterol. In present study, we have studied their effects on cholesterol biosynthesis in human cells, particularly sterol-conversion pathway. For this, both Caco-2 (intestinal mucosa) and HL-60 (promyelocytic) cell lines were incubated [14C]acetate, incorporation radioactivity into sterols was determined using HPLC detection online. Sterols containing a double bond at C-22 side chain...
Endometrial carcinoma (EC) shows intertumor heterogeneity, with several different histologic types differing in their morphologic and molecular features. There is also intratumoral neoplastic cell components within the same tumor, In this article, we discuss consequences of tumor heterogeneity EC at levels, by describing some illustrative examples produced research team. They are (1) conventional mixed tumors, (2) microsatellite instability, (3) branched evolution as shown exome sequencing...
Arsenic trioxide (As<sub>2</sub>O<sub>3</sub>) caused apoptosis in U-937 human promonocytic cells. This effect was potentiated by the simultaneous addition of glutathione (GSH) synthesis inhibitor dl-buthionine-(<i>R,S</i>)-sulfoximine or protein kinase C activators 12-<i>O</i>-tetradecanoylphorbol-13-acetate (TPA) and bryostatin 1. In TPA decreased intracellular GSH content, ERK activation, As<sub>2</sub>O<sub>3</sub>-provoked activation p38 JNK. The <i>N</i>-acetyl-l-cysteine, PKC...
Abstract Treatment with 1–4 µM As 2 O 3 slightly induced apoptosis in U‐937 human promonocitic leukemia cells. This effect was potentiated by co‐treatment MEK/ERK (PD98059, U0126) and JNK (SP600125, AS601245) inhibitors, but not p38 (SB203580, SB220025) inhibitors. However, no potentiation obtained using lonidamine, doxorubicin, or cisplatin instead of . Apoptosis mitogen‐activated protein kinase (MAPK) inhibitors involved both the intrinsic extrinsic executionary pathways, as demonstrated...
Cholesterol is essential for cell growth and division, but whether this just a consequence of its use in membrane formation or it also elicits regulatory actions cycle machinery remains to be established. Here, we report on the specificity action cholesterol human cells by comparing effects with those ergosterol, yeast sterol structurally similar cholesterol. Inhibition synthesis means SKF 104976 incubated cholesterol‐free medium resulted proliferation inhibition arrest at G2/M phase. These...
The response of anandamide is terminated by a carrier‐mediated transport followed degradation catalyzed the cloned enzyme fatty acid amidohydrolase (FAAH). In this study, we provide biochemical data showing an uptake process and expression FAAH in human prostate. Anandamide was accumulated PC‐3 cells saturable temperature‐dependent process. Kinetic studies uptake, determined presence cannabinoid vanilloid antagonists, revealed apparent parameters K M =4.7±0.2 μ V max =3.3±0.3 pmol min −1 (10...
Abstract Limited information is currently available about the proliferation activity and cell‐cycle distribution of different bone marrow (BM) cell subsets defined according to their lineage maturation stage in normal versus cytopenia‐associated reactive BM samples. Here, we report a three‐color flow cytometry approach investigate compartments—CD34 + hematopoietic progenitor precursor cells (HPC), maturing neutrophils monocytic cells, mature lymphocytes, eosinophils, nucleated red blood...
The administration of the H<sub>2</sub>O<sub>2</sub>-specific scavenger catalase attenuated generation apoptosis by antitumor drugs etoposide, camptothecin, doxorubicin, and cisplatin in U-937 human promonocytic cells. By contrast, antioxidant potentiated inducers stress response, heat shock cadmium, this other myeloid cell types. Catalase also increased shock-provoked stimulation caspase-3 -9 activities, as well release cytochrome c from mitochondria to cytosol. potentiation death...
Myelodysplastic syndromes (MDS) are clonal stem cell disorders which frequently show a hypercellular dysplastic bone marrow (BM) associated with inefficient hematopoiesis and peripheral cytopenias due to increased apoptosis maturation blockades. Currently, little is known about the role of proliferation in compensating for BM failure syndrome determining patient outcome. Here, we analyzed index (PI) different compartments hematopoietic cells 106 MDS patients compared both normal/reactive (n...