A5088485440- HIV Research and Treatment
- RNA modifications and cancer
- HIV/AIDS drug development and treatment
- T-cell and B-cell Immunology
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- DNA Repair Mechanisms
- Diffusion and Search Dynamics
- RNA Interference and Gene Delivery
- Advanced Battery Materials and Technologies
- RNA Research and Splicing
- Nanoparticles: synthesis and applications
- DNA and Nucleic Acid Chemistry
- Advanced Nanomaterials in Catalysis
- Advancements in Battery Materials
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Immune Cell Function and Interaction
- Cancer-related molecular mechanisms research
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- Cancer Genomics and Diagnostics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Monoclonal and Polyclonal Antibodies Research
- Alzheimer's disease research and treatments
South China Agricultural University
2025
University of Cambridge
2021-2025
Hunan University
2022-2024
Shenzhen University
2021-2024
Jinan University
2022-2024
University of Toronto
2020-2024
York University
2024
Second Affiliated Hospital of Xi'an Jiaotong University
2024
State Key Laboratory of Chemobiosensing and Chemometrics
2023
University of Saskatchewan
2011-2021
Cytosine mutations within TCA/T motifs are common in cancer. A likely cause is the DNA cytosine deaminase APOBEC3B (A3B). However, A3B-null breast tumours still have this mutational bias. Here we show that APOBEC3H haplotype I (A3H-I) provides a solution to paradox. with bias at least one copy of A3H-I despite little genetic linkage between these genes. Although deemed inactive previously, has robust activity biochemical and cellular assays, similar A3H-II after compensation for lower...
METTL3, a primary methyltransferase catalyzing the RNA N6-methyladenosine (m6A) modification, has been identified as an oncogene in several cancer types and thus nominated potentially effective target for therapeutic inhibition. However, current options using this strategy are limited. In study, we targeted protein-protein interactions at METTL3-METTL14 binding interface to inhibit complex formation subsequent catalysis of m6A modification. Among candidate peptides, RM3 exhibited highest...
Abstract Background Salmonella enteritidis is a prevalent foodborne pathogen causing diseases in humans and poultry globally. While clove extract known for its anti-inflammatory properties, specific effects on gut injury underlying mechanisms are not well understood. Methods A total of 432 one-day-old male fast-growing yellow-feathered broilers with similar body weight were randomly assigned to 6 groups, the CON S.E fed basal diet; CE + received 300 mg/kg their diets; EUG had 180 eugenol...
The {101}–{001} surface heterojunction constructed on polyhedral titanium dioxide (TiO2) nanocrystals has recently been proposed to be favorable for the efficient electron–hole spatial separation due preferential accumulation of electron and hole {101} {001} facets, respectively. formed free can promote reactive oxygen species (ROS) production, which potentially used inactivation bacteria. In present study, a series truncated octahedral bipyramid TiO2 (T1, T2, T3, T4) coexposed with facets...
Simulated sunlight has promise as a light source able to alleviate the severe pain associated with patients during photodynamic therapy (PDT); however, low utilization efficiency of traditional photosensitizers dramatically limits its application. Titanium‐dioxide‐nanoparticle–gold‐nanocluster–graphene (TAG) heterogeneous nanocomposites are designed efficiently utilize simulated for melanoma skin cancer PDT. The narrow band gap in gold nanoclusters (Au NCs), and staggered energy bands...
The magnetization and magnetic anisotropy of FeCo/MgO(001) thin film under electric field were investigated by the first-principles calculations. Three different interface configurations considered: Co/Fe/MgO, Fe/Co/MgO, FeCo/FeCo/MgO. It was found that perpendicular preferred for all enhanced with increasing field, which consistent experimental results. Furthermore, our calculations indicated FeCo/FeCo/MgO most stable configuration had largest energy. results also showed Fe/Co/MgO larger...
APOBEC3G is a single-stranded (ss) DNA deaminase that restricts replication of HIV-1 by inducing viral genome mutagenesis through deamination cytosine to uracil on cDNA. has polydisperse oligomeric states and deaminates ssDNA processively jumping sliding. catalytically inactive N-terminal CD1 domain mediates processivity an active C-terminal CD2 catalyzes deaminations. Here, we assess the determinants efficiency mediated comparing native two mutants, monomeric mutant (F126A/W127A) clinical...
The single-stranded DNA cytidine deaminases APOBEC3B, APOBEC3H haplotype I, and APOBEC3A can contribute to cancer through deamination of cytosine form promutagenic uracil in genomic DNA. enzymes must access during the dynamic processes replication or transcription, but enzymatic mechanisms enabling this activity are not known. To study this, we developed a method purify full length APOBEC3B characterized it comparison on substrates relevant mutagenesis. We found that ability an APOBEC3 cycle...
APOBEC3H is a deoxycytidine deaminase that can restrict the replication of HIV-1 in absence viral protein Vif induces degradation cells. exists humans as seven haplotypes (I-VII) with different cellular stabilities. Of three stable (II, V, and VII), II V occur most frequently population. Despite being bona fide restriction factor, there has been no comparative biochemical characterization haplotypes. We characterized ssDNA scanning mechanisms use to search their substrate for...
The seven APOBEC3 (A3) enzymes in primates restrict HIV/SIV replication to differing degrees by deaminating cytosine viral (−)DNA, which forms promutagenic uracils that inactivate the virus. A polymorphism human APOBEC3C (A3C) encodes an S188I mutation increases enzymatic activity of protein and its ability HIV-1, correlates with increased propensity form dimers. However, other hominid A3C proteins only have S188, suggesting they should be less active like common A3C. Nonetheless, here we...
A ternary composite solid-state electrolyte of PVDF-HFP, LATP and flower-like CeO 2 with rich oxygen vacancies is developed for the first time, it presents enhanced ionic conductivity, high transference number excellent electrochemical performance at room temperature.
Abstract METTL3, a primary methyltransferase catalyzing the RNA N6‐methyladenosine (m6A) modification, has been identified as an oncogene in several cancer types and thus nominated potentially effective target for therapeutic inhibition. However, current options using this strategy are limited. In study, we targeted protein–protein interactions at METTL3–METTL14 binding interface to inhibit complex formation subsequent catalysis of m6A modification. Among candidate peptides, RM3 exhibited...
APOBEC3G (A3G) is a human enzyme that inhibits immunodeficiency virus type 1 (HIV-1) infectivity, in the absence of viral infectivity factor Vif, through deoxycytidine deamination and deamination-independent mechanism. A3G converts from fast to slow binding state oligomerization, which suggests large oligomers could block HIV-1 reverse transcriptase-mediated DNA synthesis, thereby inhibiting replication. However, it unclear how small number molecules found form oligomers. Here we measure...
ABSTRACT The APOBEC3 deoxycytidine deaminases can restrict the replication of HIV-1 in cell culture to differing degrees. effects enzymes are largely suppressed by Vif that interacts with host proteins form a Cullin5-Ring E3 ubiquitin ligase induces 48 K-linked polyubiquitination (poly-Ub) and proteasomal degradation enzymes. variants have abilities induce underlying biochemical mechanisms for these differences is not fully understood. We hypothesized characterizing interaction multiple we...
The APOBEC3 family of cytosine deaminase enzymes are able to restrict replication retroelements, such as LINE-1. However, each the seven have been reported act differentially prevent LINE-1 retrotransposition and mechanisms APOBEC3-mediated inhibition has not well understood. prevailing view for many years was that deamination-independent relied on APOBEC3s blocking reverse transcriptase DNA polymerization or transport RNA into nucleus. recently it shown APOBEC3A can deaminate cytosine, form...
The molecular mechanisms underpinning prostate cancer (PCa) progression are incompletely understood, and precise stratification of aggressive primary PCa (pri-PCa) from indolent ones poses a major clinical challenge. Here, we comprehensively dissect, genomically transcriptomically, the m6A (N6-methyladenosine) pathway as whole in PCa. Expression, but not genomic alteration, repertoire full set 24 regulators at population level successfully stratifies pri-PCa into three clusters with distinct...
Conventional in vitro aggregation assays often involve tagging with extrinsic fluorophores, which can interfere aggregation. We propose the use of intrinsic amyloid fluorescence lifetime probed using two-photon excitation and represented by model-free phasor plots as a label-free assay to characterize structure. Intrinsic arises from structured packing β-sheets amyloids is independent aromatic-based fluorescence. show that different [i.e., α-Synuclein (αS), β-Lactoglobulin (βLG), TasA]...
Abstract METTL3 has emerged as a promising therapeutic target in cancer treatment, although its oncogenic functions melanoma development and potential for targeting drug have not been fully explored. In this study, we define the role of progression. Building on insight, examine our recently designed peptide inhibitor RM3 , which targets binding interface METTL3/14 complex disruption subsequent ubiquitin‐mediated proteasomal degradation via E3 ligase STUB1. treatment reduces proliferation,...