A5103044170- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Hematopoietic Stem Cell Transplantation
- Systemic Lupus Erythematosus Research
- Renal Transplantation Outcomes and Treatments
- Biosensors and Analytical Detection
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 detection and testing
- CAR-T cell therapy research
- Virus-based gene therapy research
- Helicobacter pylori-related gastroenterology studies
- Scientific Computing and Data Management
- Mobile Crowdsensing and Crowdsourcing
- Neurological Complications and Syndromes
- Nutrition, Genetics, and Disease
- COVID-19 epidemiological studies
- Acute Lymphoblastic Leukemia research
- COVID-19 diagnosis using AI
- CRISPR and Genetic Engineering
- Cancer Research and Treatments
- Human Health and Disease
- Celiac Disease Research and Management
- Cytomegalovirus and herpesvirus research
- Acute Myeloid Leukemia Research
- Biomedical and Engineering Education
North West London Pathology
2020-2025
Hammersmith Hospital
2003-2024
Imperial College Healthcare NHS Trust
2021-2023
Cancer Research UK
2003
Imperial College London
2003
University College London
2000-2002
The Royal Free Hospital
2000-2002
Abstract The SARS-CoV-2 pandemic has shown how a rapid rise in demand for patient and community sample testing can quickly overwhelm capability globally. With most diagnostic infrastructure dependent on specialized instruments, their exclusive reagent supplies become bottlenecks, creating an urgent need approaches to boost capacity. We address this challenge by refocusing the London Biofoundry onto development of alternative pipelines. Here, we present reagent-agnostic automated platform...
Oncolytic replication-selective adenoviruses constitute a rapidly expanding experimental approach to the treatment of cancer. However, due lack an immunocompetent and replication-competent efficacy model, role host immune response viral E3 immunoregulatory genes remained unknown. We screened nine murine carcinoma lines for adenovirus (Ad5) uptake, gene expression, replication, cytopathic effects. In seven these cell infectability effects were similar those seen with human lines....
Abstract A review of the British Society for Histocompatibility and Immunogenetics (BSHI) Guideline ‘HLA matching donor selection haematopoietic progenitor cell transplantation’ published in 2016 was undertaken by a BSHI appointed writing committee. Literature searches were performed data extracted presented as recommendations according to GRADE nomenclature.
Defining HLA mismatch at the molecular compared with antigen level has been shown to be superior in predicting alloimmune responses, although data from across different patient populations is lacking. Using HLA-Matchmaker, HLA-EMMA and PIRCHE-II, this study reports on association between (MolMM) de novo DSA (dnDSA) an ethnically diverse kidney transplant population receiving a steroid sparing immunosuppression protocol.51/419(12.2%) patients had dnDSA. De were seen more frequently males,...
HLA genes exhibit a high degree of polymorphism, contributing to genetic variability known influence immune responses infection. Here we investigate associations between polymorphism and serological T-lymphocyte the BNT162b2 ChAdOx1 SARS-CoV-2 vaccines within population receiving maintenance haemodialysis (HD) for End-Stage Renal Disease (ESRD). Our primary objective was identify alleles associated with diminished T-cellular responsiveness vaccination. As secondary objective, type COVID-19...
Systemic lupus erythematosus (SLE) is characterized by B cell hyperactivity and the production of autoantibodies, some which (antibodies to dsDNA) are thought be pathogenic. T helper cells drive autoantibodies aim this study characterize phenotypically a subpopulation (the CD3+ CD4- CD8-, double negative (DN) cells) previously identified as helping enhance anti-DNA antibodies in patients with SLE. Data were obtained using FACS staining DN that had been purified from PBMCs magnetic bead...
Systemic lupus erythematosus (SLE) is a chronic autoimmune rheumatic disease that may affect every organ or system in the body. We have shown previously TCR ab‡ subpopulation of CD3‡CD4 7 CD8 , DN T cells expanded patients with SLE and double negative express increased levels activation markers compared both healthy people rheumatoid arthritis, (RA) as controls. The aim this study was to characterize these terms their ability produce IL4, Th2 cytokine, spontaneously after mitogen...
Abstract Solid organ transplantation represents the best (and in many cases only) treatment option for patients with end‐stage failure. The effectiveness and functioning life of these transplants has improved each decade due to surgical clinical advances, accurate histocompatibility assessment. Patient exposure alloantigen from another individual is a common occurrence takes place through pregnancies, blood transfusions or previous transplantation. Such alloantigen's can lead formation...
Abstract Coeliac disease is a common immune‐mediated inflammatory disorder caused by dietary gluten in genetically susceptible individuals. While the diagnosis of coeliac based on serological and histological criteria, HLA‐DQ genotyping can be useful, especially excluding patients who do not carry relevant DQ heterodimers: DQA1*05 DQB1*02, DQB1*03:02 or DQA1*02 DQB1*02 (commonly referred to as DQ2.5, DQ8 DQ2.2, respectively). External quality assessment results for HLA have revealed...
<ns4:p><ns4:bold>Background:</ns4:bold> Diagnostic laboratories are currently required to provide routine testing of asymptomatic staff and patients as a part their clinical screening for SARS-CoV-2 infection. However, these cohorts display very different disease prevalence from symptomatic individuals capacity is often limited. Group frequently proposed possible solution address this; however, proposals neglect the technical operational feasibility implementation in front-line diagnostic...
Summary Some γδ T cells express a receptor for the Fc portion of immunoglobulin G (FcγRIII – CD16). The relevance this to T‐cell function is at present unclear. Our previous studies have shown that activation markers in patients with rheumatoid arthritis (RA). In study we examined relative proportions CD16 + blood and synovial fluid these compared control blood. from RA were significantly reduced circulation. That was due blocking antibody binding unlikely as treatment (known contain immune...
This analysis reports on the outcomes of two different steroid sparing immunosuppression protocols used in management 120 highly sensitised patients (HSPs) with cRF>85% receiving Alemtuzumab induction, 53 maintained tacrolimus (FK) monotherapy and 67 plus mycophenolate mofetil (FK + MMF). There was no difference median cRF or mode sensitisation between groups, although FK MMF cohort received more poorly matched grafts. one-year patient allograft survival, however rejection free survival...
Relapse is a major cause of treatment failure in haploidentical haematopoietic progenitor cell transplant (HPCT) with PTCy. Natural killer cells suppress graft versus host disease and mediate the leukaemia effect, driven by immunoglobulin‐like receptors (KIRs). Emerging research suggests that donor KIR genotype may influence outcome transplants varying impacts between patient cohorts. This study investigates whether donors greater B motifs associate outcomes such as relapse‐free survival...