A5010880471- Cancer-related Molecular Pathways
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Glioma Diagnosis and Treatment
- Virus-based gene therapy research
- DNA Repair Mechanisms
- Platelet Disorders and Treatments
- CAR-T cell therapy research
- Blood properties and coagulation
- Retinoids in leukemia and cellular processes
- Cell death mechanisms and regulation
- Autophagy in Disease and Therapy
- Chromium effects and bioremediation
- CRISPR and Genetic Engineering
- Nuclear Structure and Function
- Viral Infectious Diseases and Gene Expression in Insects
- Curcumin's Biomedical Applications
- Epigenetics and DNA Methylation
- FOXO transcription factor regulation
- S100 Proteins and Annexins
- Neuroblastoma Research and Treatments
- Viral-associated cancers and disorders
- Redox biology and oxidative stress
- RNA Research and Splicing
- Endoplasmic Reticulum Stress and Disease
University of Portsmouth
2013-2023
University of Algarve
2013-2023
Brain Tumour Research
2014-2023
Dalhousie University
2007-2019
Institute for Biotechnology and Bioengineering
2013-2015
Algarve Biomedical Center
2015
Hospital for Sick Children
2014
SickKids Foundation
2014
First Hospital of Jilin University
2013
Jilin University
2013
New cytotoxic agents are urgently needed for the treatment of advanced ovarian cancer because poor long-term response this disease to conventional chemotherapy. Curcumin, obtained from rhizome Curcuma longa, has potent anticancer activity; however, mechanism curcumin-induced cytotoxicity in cells remains a mystery. In study we show that curcumin exhibited time- and dose-dependent against monolayer cultures carcinoma cell lines with differing p53 status (wild-type p53: HEY, OVCA429; mutant...
To date, testing of various cytokines for the stimulation blood cell production has not demonstrated a consistent effect on peripheral platelet levels. In this report, we provide evidence that human recombinant IL-6 increased in mice, as measured by both levels and [75Se]selenomethionine (75SeM) incorporation into newly forming platelets. Peripheral white counts also were increased, but only to modest extent, hematocrit values unchanged. A dose-response relationship between amount...
Patricia Alexandra Madureira 1 , Richard Hill 2 Victoria Ann Miller Carman Giacomantonio 3 Patrick Wing Kwong Lee and David Morton Waisman Departments of Biochemistry & Molecular Biology Pathology Department Microbiology Immunology Medicine, Dalhousie University Halifax, Nova Scotia, B3H 4R2, Canada Received: December 1, 2011; Accepted: 9, Published: 20, Keywords: ANXA2, reactive oxygen species (ROS), redox regulation, oxidative stress, tumorigenesis Correspondence: Waisman, email: //...
Abstract Intrinsic and acquired resistance to chemotherapy is the fundamental reason for treatment failure many cancer patients. The identification of molecular mechanisms involved in drug or sensitization imperative. Here we report that tribbles homologue 2 (TRIB2) ablates forkhead box O activation disrupts p53/MDM2 regulatory axis, conferring various chemotherapeutics. TRIB2 suppression exerted via direct interaction with AKT a key signalling protein cell proliferation, survival metabolism...
Oncolytic replication-selective adenoviruses constitute a rapidly expanding experimental approach to the treatment of cancer. However, due lack an immunocompetent and replication-competent efficacy model, role host immune response viral E3 immunoregulatory genes remained unknown. We screened nine murine carcinoma lines for adenovirus (Ad5) uptake, gene expression, replication, cytopathic effects. In seven these cell infectability effects were similar those seen with human lines....
p21(Cip1/WAF1) is the principle mediator of cell cycle arrest in response to DNA damage. p21 primarily mediates G(1) by inactivating G(1)-associated cyclin A- and E-containing cyclin/cdk complexes. In present study we investigate role damage-induced G(2) arrest, particularly with respect G(2)-associated cyclin, B1. We demonstrate that cells lacking or deficient their ability upregulate are unable mediate downregulation B1 damage as compared wild-type cells. Decreased levels seen due...
Gemcitabine is a chemotherapeutic that widely used for the treatment of variety haematological malignancies and has become standard chemotherapy advanced pancreatic cancer. Combinational gemcitabine regimes (e.g.with doxorubicin) are being tested in clinical trials to treat cancers, including colon The limited success these prompted us pursue better understanding gemcitabine's mechanism cell killing, which could dramatically improve therapeutic potential this agent. For comparison, we...
Glioblastoma (GB) is the most common and deadly type of primary malignant brain tumor with an average patient survival only 15–17 months. GBs typically have hypoxic regions associated aggressiveness chemoresistance. Using derived GB cells, we characterized how responds to hypoxia. We noted a hypoxia-dependent glycolytic switch by up-regulation HK2, PFKFB3, PFKFB4, LDHA, PDK1, SLC2A1/GLUT-1, CA9/CAIX, SLC16A3/MCT-4. Moreover, many proangiogenic genes proteins, including VEGFA, VEGFC, VEGFD,...
Annexin A2 is an abundant cellular protein that mainly localized in the cytoplasm and plasma membrane, however a small population has been found nucleus, suggesting nuclear function for protein. possesses export sequence (NES) inhibition of NES sufficient to cause accumulation. Here we show annexin accumulates nucleus response genotoxic agents including gamma-radiation, UV radiation, etoposide chromium VI this event mediated by A2. Nuclear accumulation blocked antioxidant agent N-acetyl...
Abstractp53 is a tumor suppressor that integrates signals from different stress induced signalling pathways, regulates cell cycle arrest, senescence, apoptosis, and DNA repair. How p53 dictates fate unclear. As major transcriptional target of in response to cellular stress, p21 key component control directing an anti-apoptotic following damage. It therefore likely p53-dependent regulation contributes, at least part, how influences outcome upon Here we compare the damage by ultraviolet (UV)...
Naturally oncolytic reovirus preferentially kills cancer cells, making it a promising therapeutic. Mutations in tumour suppressor p53 are prevalent cancers, yet the role of oncolysis is relatively unexplored. Human cell lines were exposed to Nutlin-3a, or combination two and cells processed for titration, western blot, real-time PCR apoptosis assay using Annexin V 7-AAD staining. Confocal microscopy was used determine translocation NF-κB p65 subunit. We show that despite similar replication...
Richard Hill 1 , Patricia A. Madureira 2 David M. Waisman and Patrick W.K. Lee 1,3 Department of Microbiology & Immunology, Dalhousie University, Halifax, Nova Scotia, Canada Biochemistry Molecular Biology, 3 Pathology, Received: December 8, 2011; Accepted: 9, Published: 20, Keywords: DNA-PKCs, p53, p21 transcription suppression Correspondence: Lee, email: // Abstract A key determinant p53-mediated cell fate following various DNA damage modalities is WAF1/CIP1 expression, with elevated...
The activation of the phosphoinositide 3-kinase (PI3K)/AKT signalling pathway is one most frequent genetic events in breast cancer, consequently development PI3K inhibitors has attracted much attention. Here we evaluate effect inhibition on global gene expression cancer cells. We used a range methodologies that include silico compound analysis, vitro kinase assays, cell invasion proliferation genome-wide transcription studies (Agilent Technologies full genome arrays), set enrichment...
Malignant melanoma is the most deadly form of skin cancer. There a critical need to identify patients that could be successfully treated by surgery alone and those require adjuvant treatment. In this study, we demonstrate expression tribbles2 (TRIB2) strongly correlates with both presence progression melanocyte-derived malignancies. We examined TRIB2 in addition 12 previously described biomarkers across three independent full genome microarray studies. was consistently significantly...