A5102002771- Mesenchymal stem cell research
- Endodontics and Root Canal Treatments
- Periodontal Regeneration and Treatments
- Dental Radiography and Imaging
- Dental Trauma and Treatments
- 3D Printing in Biomedical Research
- Pulmonary Hypertension Research and Treatments
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Drilling and Well Engineering
- Dental Anxiety and Anesthesia Techniques
- Pluripotent Stem Cells Research
- Oral microbiology and periodontitis research
- Urological Disorders and Treatments
- Organ Transplantation Techniques and Outcomes
- Organ Donation and Transplantation
- dental development and anomalies
- Laser Applications in Dentistry and Medicine
- Dental Health and Care Utilization
- Oral Health Pathology and Treatment
- Health disparities and outcomes
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- Bone and Dental Protein Studies
Stomatological Hospital of Chongqing Medical University
2012-2025
Chongqing Medical University
2016-2025
The Affiliated Yongchuan Hospital of Chongqing Medical University
2018-2024
China Medical University
2024
First Hospital of China Medical University
2024
Southern University of Science and Technology
2023-2024
Wake Forest University
2019-2020
Forest Institute
2019-2020
University of Pittsburgh
2014
Sichuan University
2013
Dental pulp stem cell-derived exosomes (DPSC-EXO), which have biological characteristics similar to those of metrocytes, been found be closely associated with tissue regeneration. Periodontitis is an immune inflammation and destructive disease caused by plaque, resulting in alveolar bone loss periodontal epithelial destruction. It not clear whether DPSC-EXO can used as effective therapy for The purpose this study was only verify the effect on reducing periodontitis promoting regeneration,...
The aim is to investigate the prevalence of dental anxiety and its association with pain other related factors in adult patients irreversible pulpitis. One hundred thirty pulpitis were included this cross-sectional study. Participants asked fill out an information table a battery questionnaires assess their level anxiety, at first most recent experience, intensity before/during present endodontic treatment. that participants displayed during treatment was also evaluated by dentists using...
To evaluate root canal morphology of permanent mandibular incisor teeth in a Chinese population using cone-beam computed tomography (CBCT).CBCT images patients were collected and 1553 incisors the included. The following observations on included to determine number roots, configuration. configurations classified. effect gender incidence second was investigated.Of incisors, 86.8% had single with canal. Mandibular lateral (17.5%) higher compared central (8.9%) (p = 0.000). A slightly...
This study was to investigate root anatomy and canal morphology of mandibular first premolars in a Chinese population. 178 human permanent extracted from native population were collected, scanned using micro-computed tomography reconstructed three-dimensionally. The number roots canals, configuration radicular grooves investigated. categorized according Vertucci's criteria. scored the Arizona State University dental anthropology scoring system (ASUDAS), correlation between scores for...
The development of human cell-based systems to replace the use rodents or two-dimensional culture cells for studying nephrotoxicity is urgently needed. Human urine-derived stem were differentiated into renal tubular epithelial in three-dimensional (3-D) after being induced by a kidney extracellular matrix. Levels CYP2E1 and KIM-1 3-D organoids significantly increased response acetone cisplatin. This system provides an alternative tool screening research.
Thanks to the advantages of easy harvesting and escape from immune rejection, autologous bone marrow-derived mesenchymal stem cells (BMSCs) are promising candidates for immunosuppressive therapy against inflammation autoimmune diseases. However, is still challenging because immunomodulatory properties BMSCs always impaired by immunopathogenesis in patients. Because its reliable extensive biological activities, osthole has received increased clinical attention. In this study, we found that...
To establish a 3D model for screening the biocompatibility of dental materials/drugs on pulp cells and tissue.Human (hDPC) endothelial (EC) were mixed with or without human derived extracellular matrix (hDP-ECM) according to several protocols cultured in plates fabricate organoids. Cell viability proliferation organoids evaluated using Live/Dead cell assay ATPase assay. Organoids fixed, cut stained H&E staining kit. The expressions DSPP, DMP-1, CD31, vWF COL1A immunofluorescence. assess...
The aim was to investigate the angiogenic effects of concentrated growth factors on human dental pulp cells and umbilical vein endothelial cells. Cells were treated with factor extracts. CCK-8 assay cell cycle conducted evaluate growth. Cell migration evaluated by Transwell assay. Angiogenesis-associated mRNA protein expression levels determined using quantitative real-time PCR Western blotting, respectively. A tube formation capacity in vitro. data showed that compared control, extracts...
The aim of the present study was to investigate effect calcium hydroxide [Ca(OH)2], mineral trioxide aggregate (MTA), iRoot BP, platelet-rich fibrin (PRF) and concentrated growth factors (CGF) on proliferation, viability, apoptosis mineralization human dental pulp cells (HDPCs). HDPCs were treated with Ca(OH)2, MTA, PRF CGF exudates. Cell apoptosis, cell cycle alkaline phosphatase (ALP) activity evaluated in vitro. significantly increased proliferation as compared that MTA BP groups day 3....
To investigate the effect of supplemental lingual infiltration (LI) mandibular molars following an inferior alveolar nerve block (IANB) plus buccal (BI) in patients with irreversible pulpitis.Eighty adult diagnosed pulpitis participated this prospective study. All received standard IANB via injection 4 mL 2% lidocaine 1 : 100,000 epinephrine. Ten minutes after IANB, numbness lower lip were randomly divided into two groups. In BI group, 40 0.9 4% articaine (BLI) epinephrine and, subsequently,...
Abstract Both diabetes and periodontal disease are prevalent in China. Poor oral hygiene practice is the major cause of disease. An association between blood glucose level was reported individuals with diabetes, but not general population. We examined a population-based random survey recruiting 2,105 adults without previously diagnosed Chongqing city, Plasma hemoglobin A1c (HbA1c) were measured, 2-hour tolerance test conducted for each respondent. Self-reported toothbrushing frequency used...
Abstract Hypoxic preconditioning is commonly applied to enhance mesenchymal stem cells (MSCs) therapeutic effect before transplantation. Elucidating the of hypoxic would be beneficial for improved application. However, influence hypoxia on dental tissue derived MSCs cultured in 3D was unknown. Thus, present study investigate gene expression and proteome pulp (DPSCs) after preconditioning. DPSCs were isolated, a system under normoxic conditions. The examined with reverse transcription...
Human placental stem cells (PSCs) enhance histological and functional recovery in a rodent erectile dysfunction (ED) model. We tested the hypothesis that bioactive factors secreted by PSC (i.e., secretome) mediate acellular-conditioned media (CM) from culture (PSC-CM) could be used independently to facilitate recovery. To identify relative efficacy of PSC, comparison CM three additional human cell populations was performed. amniotic fluid (AFSCs), adipose-derived (ADSC), umbilical vein...
Secretomes produced by mesenchymal stromal cells (MSCs) were considered to be therapeutic potential. However, harvesting enough primary MSCs from tissue was time-consuming and costly, which impeded the application of secretomes. This study immortalize compare secretomes profile immortalized original MSCs. Human dental follicle (DFCs) isolated using pMPH86. The secretome DFCs (iDFCs) investigated compared iTRAQ labeling combined with mass spectrometry (MS) quantitative proteomics. MS data...
This study investigated the safety of a novel cell-labeling technology with mKATE and Renilla reniformis luciferase (mKATE-renLUC) assessed efficacy on tracking implanted human placental stromal cells (PSC) in an erectile dysfunction (ED) animal model. Human PSC were labeled mKATE-renLUC using lentivirus. Cell viability, apoptosis, proliferation, migration, surface marker expression differentiation potential evaluated compared non-labeled PSC. The paracrine profile was examined angiogenesis...
To compare the efficiency of conventional needle irrigation, passive ultrasonic sonic irrigation and XP-endo Finisher in removing Vitapex paste from root canals. The canals human single-rooted teeth were prepared obturated with paste. After 2 weeks, 48 randomly allocated into four groups: (CNI) group, (PUI) (SI) group (XP) group. specimens groups scanned using a micro-computed tomography after pastes was removed. results showed that more residual left CNI than other three (p < 0.05). There...