A5008630157- Microbial Inactivation Methods
- Microfluidic and Bio-sensing Technologies
- Transgenic Plants and Applications
- Sphingolipid Metabolism and Signaling
- RNA Interference and Gene Delivery
- Click Chemistry and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Nanoparticle-Based Drug Delivery
- Peptidase Inhibition and Analysis
- CRISPR and Genetic Engineering
- Immune cells in cancer
- Toxin Mechanisms and Immunotoxins
- Magnetic and Electromagnetic Effects
- Protein Degradation and Inhibitors
- Nanoplatforms for cancer theranostics
- Virus-based gene therapy research
- Cancer, Lipids, and Metabolism
- Plasma Applications and Diagnostics
- 3D Printing in Biomedical Research
- Advanced biosensing and bioanalysis techniques
- Cancer Research and Treatments
- Metabolism, Diabetes, and Cancer
- Plant Virus Research Studies
- Cancer therapeutics and mechanisms
- Lipid Membrane Structure and Behavior
Université de Toulouse
2016-2025
Université Toulouse III - Paul Sabatier
2015-2025
Centre National de la Recherche Scientifique
2016-2025
Institut de Pharmacologie et de Biologie Structurale
2016-2025
Institut Claudius Regaud
2011
Hôpital Rangueil
2010
Imperial College London
2010
Inserm
2010
Weatherford College
2009
Centre National pour la Recherche Scientifique et Technique (CNRST)
2008
Electropermeabilization is one of the nonviral methods successfully used to transfer genes into living cells in vitro and vivo . Although this approach shows promise field gene therapy, very little known about basic processes supporting DNA transfer. The present investigation studies process at single-cell level by using digitized fluorescence microscopy. Permeabilization a prerequisite for Its assay propidium-iodide (PI) penetration that it occurs sides cell membrane facing two electrodes,...
Abstract Malignant progression results from a dynamic cross-talk between stromal and cancer cells. Recent evidence suggests that this is mediated to significant extent by exosomes, nanovesicles secreted most cell types which allow the transfer of proteins, lipids, nucleic acids Adipocytes are major component several tumor microenvironments, including invasive melanoma, where cells have migrated adipocyte-rich hypodermic layer skin. We show adipocytes secrete exosomes in abundance, then taken...
Obesity favours the occurrence of locally disseminated prostate cancer in periprostatic adipose tissue (PPAT) surrounding gland. Here we show that adipocytes from PPAT support directed migration cells and this event is strongly promoted by obesity. This process dependent on secretion chemokine CCL7 adipocytes, which diffuses to peripheral zone prostate, stimulating CCR3 expressing tumour cells. In obesity, higher facilitates extraprostatic extension. The observed increase associated with...
Abstract Systemic chemotherapy was considered of modest efficacy in prostate cancer until the recent introduction taxanes. We took advantage known differential effect camptothecin and docetaxel on human PC-3 LNCaP cells to determine their sphingosine kinase-1 (SphK1) activity subsequent ceramide/sphingosine 1-phosphate (S1P) balance relation with cell survival. In vitro, induced strong inhibition SphK1 elevation ceramide/S1P ratio only lines sensitive these drugs. overexpression both...
Gene electrotransfer is gaining momentum as an efficient methodology for nonviral gene transfer. In skeletal muscle, data suggest that electric pulses play two roles: structurally permeabilizing the muscle fibers and electrophoretically supporting migration of DNA toward or across permeabilized membrane. To investigate this further, combinations short high-voltage (HV; hundreds V/cm) mainly electrophoretic long low-voltage (LV; tens were investigated in liver, tumor, skin rodent models. The...
Gene electrotransfer is gaining momentum as an efficient methodology for nonviral gene transfer. In skeletal muscle, data suggest that electric pulses play two roles: structurally permeabilizing the muscle fibers and electrophoretically supporting migration of DNA toward or across permeabilized membrane. To investigate this further, combinations short high-voltage (HV; hundreds V/cm) mainly electrophoretic long low-voltage (LV; tens were investigated in liver, tumor, skin rodent models. The...
Radiotherapy is widely used as a radical treatment for prostate cancer, but curative treatments are elusive poorly differentiated tumors where survival just 15% at 15 years. Dose escalation improves local response rates limited by tolerance in normal tissues. A sphingosine analogue, FTY720 (fingolimod), drug currently phase III studies of multiple sclerosis, has been found to be potent apoptosis inducer cancer cells. Using vitro and vivo approaches, we analyzed the impact on sphingolipid...
The RNA interference-mediated gene silencing approach is promising for therapies based on the targeted inhibition of disease-relevant genes. Electropermeabilization one nonviral methods successfully used to transfer siRNA into living cells in vitro and vivo. Although this effective field by interference, very little known about basic processes supporting transfer. In study, we investigated, direct visualization at single-cell level, delivery Alexa Fluor 546-labeled murine melanoma stably...
Abstract We have previously reported that, in prostate cancer, inhibition of the oncogenic sphingosine kinase-1/sphingosine 1-phosphate (SphK1/S1P) pathway is a key element chemotherapy-induced apoptosis. Here, we show that selective pharmacologic SphK1 triggers apoptosis LNCaP and PC-3 cancer cells, an effect reversed by enforced expression. More importantly, for first time up-regulation SphK1/S1P plays crucial role resistance cells to chemotherapy. Importantly, with B-5354c compound...
Electroporation is a physical method used to transfer molecules into cells and tissues. Clinical applications have been developed for antitumor drug delivery. trials of gene electrotransfer are under investigation. However, knowledge about how DNA enters not complete. By contrast small that direct access the cytoplasm, forms long lived complex with plasma membrane transferred cytoplasm considerable delay.To increase our understanding key step DNA/membrane formation, we investigated...
Summary Reasons for performing study: Sarcoids are the commonest form of equine skin tumour. Several therapeutic measures have been described but none is considered to be universally effective. Electrochemotherapy (ECT) a new anticancer therapy that utilises electrical field pulses induce increased cell membrane permeability antitumour hydrophilic drugs, such as cisplatin. The intracellular concentration drugs has significant benefit. procedure not previously reported in large number horses....
Cold atmospheric plasma-exposed culture medium may efficiently kill cancer cells in vitro. Due to the complexity of obtained after plasma exposure, less complex physiological liquids, such as saline solutions and buffers, are gathering momentum. Among plethora reactive oxygen nitrogen species (RONS) that produced these plasma-activated hydrogen peroxide, nitrite nitrate appear be mainly responsible for cytotoxic genotoxic effects. Here, we evaluated anti-cancer potential phosphate-buffered...