A5039513096- Microbial Inactivation Methods
- Microfluidic and Bio-sensing Technologies
- Magnetic and Electromagnetic Effects
- Transgenic Plants and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Toxin Mechanisms and Immunotoxins
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Lipid Membrane Structure and Behavior
- Plasma Applications and Diagnostics
- Electrohydrodynamics and Fluid Dynamics
- Electromagnetic Fields and Biological Effects
- Bacteriophages and microbial interactions
- Nanopore and Nanochannel Transport Studies
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Planarian Biology and Electrostimulation
- Microwave and Dielectric Measurement Techniques
- Photodynamic Therapy Research Studies
- Nanoplatforms for cancer theranostics
- bioluminescence and chemiluminescence research
- Ultrasound and Cavitation Phenomena
- Plant Genetic and Mutation Studies
Institut de Pharmacologie et de Biologie Structurale
2016-2025
Université Toulouse III - Paul Sabatier
2015-2025
Centre National de la Recherche Scientifique
2015-2025
Université de Toulouse
2015-2025
Laboratoire d'Analyse et d'Architecture des Systèmes
2013-2014
Centre National pour la Recherche Scientifique et Technique (CNRST)
1989-2009
Bielefeld University
1994
Institut de Biochimie et Génétique Cellulaires
1986-1992
Laboratoire de Microbiologie et Génétique Moléculaires
1989
Electropermeabilization is one of the nonviral methods successfully used to transfer genes into living cells in vitro and vivo . Although this approach shows promise field gene therapy, very little known about basic processes supporting DNA transfer. The present investigation studies process at single-cell level by using digitized fluorescence microscopy. Permeabilization a prerequisite for Its assay propidium-iodide (PI) penetration that it occurs sides cell membrane facing two electrodes,...
Abstract The integrity and morphology of bacteria is sustained by the cell wall, target main microbial inactivation processes. One promising approach to based on use pulsed electric fields (PEF). current dogma that irreversible membrane electro-permeabilisation causes death bacteria. However, actual effect cell-wall architecture has been poorly explored. Here we combine atomic force microscopy electron study organization living Bacillus pumilus at nanoscale. For vegetative bacteria, exposure...
Gene electrotransfer is gaining momentum as an efficient methodology for nonviral gene transfer. In skeletal muscle, data suggest that electric pulses play two roles: structurally permeabilizing the muscle fibers and electrophoretically supporting migration of DNA toward or across permeabilized membrane. To investigate this further, combinations short high-voltage (HV; hundreds V/cm) mainly electrophoretic long low-voltage (LV; tens were investigated in liver, tumor, skin rodent models. The...
Gene electrotransfer is gaining momentum as an efficient methodology for nonviral gene transfer. In skeletal muscle, data suggest that electric pulses play two roles: structurally permeabilizing the muscle fibers and electrophoretically supporting migration of DNA toward or across permeabilized membrane. To investigate this further, combinations short high-voltage (HV; hundreds V/cm) mainly electrophoretic long low-voltage (LV; tens were investigated in liver, tumor, skin rodent models. The...
The RNA interference-mediated gene silencing approach is promising for therapies based on the targeted inhibition of disease-relevant genes. Electropermeabilization one nonviral methods successfully used to transfer siRNA into living cells in vitro and vivo. Although this effective field by interference, very little known about basic processes supporting transfer. In study, we investigated, direct visualization at single-cell level, delivery Alexa Fluor 546-labeled murine melanoma stably...
Background Calcium electroporation describes the use of high voltage electric pulses to introduce supraphysiological calcium concentrations into cells. This promising method is currently in clinical trial as an anti-cancer treatment. One very important issue relation between tumor cell kill efficacy–and normal sensitivity. Methods Using a 3D spheroid culture model we have tested effect and electrochemotherapy using bleomycin on three different human cancer lines: colorectal adenocarcinoma...
Chinese hamster ovary (CHO) cells were reversibly permeabilized by submitting them to short, high-intensity, square wave pulses (1.8 kV/cm, 100 microseconds). The remained in a permeable state without loss of viability for several hours at 4 degrees C. A new anisotropic peak with respect control was observed on 31 P NMR spectroscopic analysis the phospholipid components. This is only present when are permeable, and normal anisotropy recovered after resealing. Taking into account fusogenicity...