A5046062964- Toxin Mechanisms and Immunotoxins
- Acute Lymphoblastic Leukemia research
- Bone Metabolism and Diseases
- Transgenic Plants and Applications
- Traumatic Brain Injury and Neurovascular Disturbances
- CAR-T cell therapy research
- Neuroscience and Neuropharmacology Research
- Bone health and treatments
- Traumatic Brain Injury Research
- Immune Cell Function and Interaction
- Osteoarthritis Treatment and Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Neuropeptides and Animal Physiology
- Adipose Tissue and Metabolism
- Periodontal Regeneration and Treatments
- Epilepsy research and treatment
- Cell death mechanisms and regulation
- Protease and Inhibitor Mechanisms
- Ion channel regulation and function
- Neuroscience and Neural Engineering
- Medical Imaging Techniques and Applications
- Receptor Mechanisms and Signaling
- Cell Adhesion Molecules Research
- Plant tissue culture and regeneration
- Trace Elements in Health
The University of Melbourne
2010-2020
Australian Institute for Musculoskeletal Science
2017-2020
Western Health
2018-2020
Victoria University
2017-2018
Sunshine Hospital
2017-2018
St Vincent's Hospital
2011-2017
Victoria School of Management
2017
The Royal Melbourne Hospital
1993-2014
Weatherford College
2011
St. Vincent's Birmingham
2011
Fat mass is an important determinant of bone density, but the mechanism this relationship uncertain. Leptin, as a circulating peptide adipocyte origin, potential contributor to relationship. Recently it was shown that intracerebroventricular administration leptin associated with loss, suggesting obesity should be low mass, opposite what actually found. Since originates in periphery, examination its direct effects on necessary address major discrepancy. Leptin (>10(-11) m) increased...
Abstract Originally, leptin was described as a product of adipocytes that acts on the hypothalamus to regulate appetite. However, subsequently, it has been shown receptors are distributed widely and diverse functions, including promotion hemopoietic osteoblastic differentiation. It recognized for some time both serum bone mass correlated positively body fat and, recently, we have direct positive relationship between in nonobese women. We now report inhibits osteoclast generation cultures...
B-cell precursor (BCP) leukemia is the most common form of childhood cancer and second acute in adults. Human BCP was treated a severe combined immunodeficient mouse model by targeting tyrosine kinase inhibitor Genistein (Gen) to B cell-specific receptor CD19 with monoclonal antibody B43. The B43-Gen immunoconjugate bound high affinity cells, selectively inhibited CD19-associated kinases, triggered rapid apoptotic cell death. At less than one-tenth maximum tolerated dose more 99.999 percent...
Very little is known regarding the effects of ionizing radiation on cytoplasmic signal transduction pathways. Here, we show that induces enhanced tyrosine phosphorylation multiple substrates in human B-lymphocyte precursors. This response to was also observed cells pretreated with vanadate, a potent protein-tyrosine-phosphatase (PTPase) inhibitor, and phosphotyrosyl [Val5]angiotensin II phosphatase assays showed no decreased PTPase activity irradiated cells. Thus, precursors not triggered by...
Absorption-contrast x-ray imaging serves to visualize the variation in attenuation within volume of a given sample, whereas phase contrast allows one variations refractive index. The former mechanism has been well known and widely utilized since time Röntgen’s Nobel prize winning work, latter mechanism—sought for, but not found, by Röntgen himself—has laid foundation for revolution which is central topic this review. We consider physical mechanisms underlying both absorption contrast,...
Summary Purpose Posttraumatic epilepsy ( PTE ) occurs in a proportion of traumatic brain injury TBI cases, significantly compounding the disability, and risk death for sufferers. To date, predictive biomarkers have not been identified. This study used lateral fluid percussion LFPI rat model to investigate whether structural, functional, behavioral changes post‐ relate later development . Methods Adult male W istar rats underwent or sham injury. Serial magnetic resonance MR positron emission...
Infrapatellar fat pad adipose stem cells (IPFP-ASCs) have been shown to harbor chondrogenic potential. When combined with 3D polymeric structures, the provide a source of engineer tissues for cartilage repair. In this study, we human IPFP-ASCs seeded onto printed chitosan scaffolds can undergo chondrogenesis using TGFβ3 and BMP6. By week 4, pearlescent, cartilage-like matrix had formed that penetrated top layers scaffold forming ‘cap’ on scaffold. Chondrocytic morphology showed typical...
Abstract Breast cancer metastasis to the bone occurs frequently, causing numerous complications including severe pain, fracture, hypercalcemia, and paralysis. Despite its prevalence severity, few effective therapies exist. To address this, we examined whether heat shock protein 90 (Hsp90) inhibitor, 17-allylamino-17-demethoxygeldanamycin (17-AAG), would be efficacious in inhibiting breast bone. Utilizing human subline, MDA-MB-231SA, previously vivo selected for enhanced ability generate...
Abstract —Modification of low density lipoprotein (LDL), eg, by oxidation, has been proposed as being important for the formation foam cells and therefore development atherosclerotic plaques. There are a number reports showing that macrophage-derived can proliferate in both human animal lesions, particularly early phase disease possibly involving macrophage-colony stimulating factor (M-CSF, or CSF-1). We studied vitro effects oxidized LDL (ox-LDL) on murine bone marrow–derived macrophages...
Mood disturbances, including depression and anxiety disorders, are common disabling long-term sequelae of traumatic brain injury (TBI). These psychiatric conditions have generally been considered psychosocial consequences the trauma, but neurobiological alterations causes also implicated. Using a rat model TBI (lateral fluid-percussion injury), this longitudinal study seeks to assess depression-like behaviors following experimental TBI. Male Wistar rats (n = 20) received severe...
Ionizing radiation triggers a signal in human B-lymphocyte precursors that is intimately linked to an active protein-tyrosine kinase regulatory pathway. We show precursors, irradiation with gamma-rays leads (i) stimulation of phosphatidylinositol turnover; (ii) downstream activation by covalent modification multiple serine-specific protein kinases, including C; and (iii) nuclear factor kappa B. All the radiation-induced signals were effectively prevented inhibitors genistein herbimycin A....
We present evidence that the CD19 receptor is functionally operative and transmits pleiotropic signals throughout pro-B, pre-pre-B, pre-B, early B, mature B cell stages of human B-cell ontogeny. The signaling ability does not depend on existence a functional antigen complex (ARC). In precursors (BCP) lacking ARC, physically associated with Src family protein tyrosine kinases (PTK). engagement BCP high affinity anti-CD19 monoclonal antibody (mAb) or its homoconjugate rapidly activates PTK...
Traumatic brain injury (TBI) has a high incidence of long-term neurologic and neuropsychiatric morbidity. Metabolic structural changes in rat brains were assessed after TBI using serial <sup>18</sup>F-FDG PET 3-dimensional MRI vivo. <b>Methods:</b> Rats underwent lateral fluid percussion (FPI; <i>n</i> = 16) or sham procedure (<i>n</i> 11). MR images acquired at 1 wk 1, 3, 6 mo injury. Morphologic MRI-based regions interest, hippocampal shape with large-deformation high-dimensional mapping....
AMP-activated protein kinase (AMPK) has been extensively studied in whole muscle biopsy samples of humans, yet the fiber type-specific expression and/or activation AMPK is unknown. We examined basal and exercise AMPK-alpha Thr(172) phosphorylation subunit (alpha(1), alpha(2), gamma(3)) type I, IIa, IIx fibers human skeletal before after 10 days training. Before training was greatest IIa (P < 0.05 vs. I IIx), while an acute bout increased all 0.05), with increase occurring fibers. Exercise...
Studies report that <sup>11</sup>C-flumazenil (FMZ) PET more specifically localizes the epileptogenic zone in patients with medically refractory focal epilepsy than <sup>18</sup>F-FDG PET. However, practical aspects of <sup>11</sup>C use limit clinical application. We a phase I/IIa study assessing <sup>18</sup>F-FMZ for localization drug-resistant temporal lobe (TLE). Receptor binding was quantified using kinetic modeling did not require arterial sampling. <b>Methods:</b> Dynamic and static...