A5081940051- Bone Metabolism and Diseases
- Bone health and treatments
- Osteoarthritis Treatment and Mechanisms
- Bone health and osteoporosis research
- Cellular Mechanics and Interactions
- RNA Research and Splicing
- Bone fractures and treatments
- Knee injuries and reconstruction techniques
- Genomics and Chromatin Dynamics
- Genetics, Bioinformatics, and Biomedical Research
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- Cell Adhesion Molecules Research
- Spaceflight effects on biology
- Endoplasmic Reticulum Stress and Disease
- Ubiquitin and proteasome pathways
- Protease and Inhibitor Mechanisms
- Wnt/β-catenin signaling in development and cancer
- Cancer Cells and Metastasis
- interferon and immune responses
- Semantic Web and Ontologies
- RNA regulation and disease
- Animal Genetics and Reproduction
- Cancer Research and Treatments
- Connective tissue disorders research
Indiana University – Purdue University Indianapolis
2016-2025
Indiana University School of Medicine
2015-2025
University of Indianapolis
2016-2025
Purdue University in Indianapolis
2025
Indiana University Health
2019-2025
Indiana University Melvin and Bren Simon Comprehensive Cancer Center
2023-2025
Indiana Hemophilia and Thrombosis Center
2020-2025
Toyo University
2024
Indiana University Indianapolis
2024
Indiana University
2008-2023
Fluorescence in situ hybridization data on distances between defined genomic sequences are used to construct a quantitative model for the overall geometric structure of human chromosome. We suggest that large-scale geometry during G0/G1 part cell cycle may consist flexible chromatin loops, averaging approximately 3 million bp, with random-walk backbone. A fully explicit, three-parametric polymer this random-walk/giant-loop can account well data. More general models consistent briefly discussed.
We determined the folding of chromosomes in interphase nuclei by measuring distance between points on same chromosome. Over 25,000 measurements were made G0/G1 DNA sequences separated 0.15-190 megabase pairs (Mbp) three human chromosomes. The specifically labeled fluorescence situ hybridization. relationship mean-square and genomic separation has two linear phases, with a transition at approximately 2 Mbp. This biphasic indicates existence organizational levels scales > 100 kbp. On...
Abstract Bone loss in postmenopausal osteoporosis is induced chiefly by an imbalance of bone-forming osteoblasts and bone-resorbing osteoclasts. Salubrinal a synthetic compound that inhibits de-phosphorylation eukaryotic translation initiation factor 2 alpha (eIF2α). Phosphorylation eIF2α alleviates endoplasmic reticulum (ER) stress, which may activate autophagy. We hypothesized signaling regulates bone homeostasis promoting autophagy inhibiting osteoclast development. To test the...
Abstract Breast cancer is the most common detected in women and current screening methods for disease are not sensitive. Volatile organic compounds (VOCs) include endogenous metabolites that provide information about health which might be useful to develop a better method breast cancer. The goal of this study was classify mice with without tumors compare localized mammary pad tumor cells injected into iliac artery by differences VOCs urine. After 4T1.2 were BALB/c either or artery, urine...
Osteoarthritis (OA) is a disease characterized by cartilage damage and abnormal remodeling of subchondral bone. Our previous study showed that in the early stage OA, knee loading exerts protective effects suppressing osteoclastogenesis through Wnt signaling, but little known about at late OA stage. Endoplasmic reticulum (ER) stress autophagy are to be involved We determined mechanical on ER mice. One hundred seventy-four mice were used for surgery-induced model. In first set experiments, 60...
Abstract Osteocytes are mechanosensitive bone cells, but little is known about their effects on tumor cells in response to mechanical stimulation. We treated breast cancer with osteocyte-derived conditioned medium (CM) and fluid flow-treated (FFCM) 0.25 Pa 1 shear stress. Notably, CM FFCM at induced the mesenchymal-to-epithelial transition (MET), epithelial-to-mesenchymal (EMT). This suggested that of flow media depend intensity. Fluorescence resonance energy transfer (FRET)-based evaluation...
Exosomes are important transporters of miRNAs, which play varying roles in the healing bone fracture.Angiogenesis is one such critical events healing, and we previously reported stimulatory effect mechanical loading vessel remodeling.Focusing on type H vessels exosomal miR-214-3p, this study examined mechanism loading-driven angiogenesis.MiRNA sequencing qRT-PCR revealed that miR-214-3p was increased exosomes bone-losing ovariectomized (OVX) mice, while it significantly decreased by knee...
Background: Bone is a frequent site of metastases from breast cancer, but existing therapeutic options are not satisfactory. Although osteoblasts have active roles in cancer progression by assisting the vicious bone-destructive cycle, we employed counterintuitive approach activating pro-tumorigenic Wnt signaling and examined paradoxical possibility developing osteoblast-derived tumor-suppressive, bone-protective secretomes. Methods: was activated overexpression Lrp5 β-catenin as well...
To investigate the therapeutic effects of static magnetic field (SMF) and its regulatory mechanism in repair osteoarthritic cartilage.Fourteen-week-old female C57BL/6 mice were randomly divided into sham operation group osteoarthritis (OA) groups with without SMF application. was applied at 200 mT for two consecutive weeks. Changes knee cartilage examined by histomorphometry, chondrogenesis migration endogenous stem cells assessed. The expression SRY-related protein 9 (SOX9), Collagen type...
CITED2 (CBP/p300-interacting transactivator with ED-rich tail 2) is a member of the Cited family nuclear regulators, previously known as mrg1 (melanocyte-specific gene-related gene 1). inducible by varying stimuli including lipopolysaccharide, hypoxia, and cytokines such interleukin 9 interferon γ. Using immortalized human chondrocyte cell line, C-28/I2, we investigated whether could be responsive to mechanical stimuli, if so, mediate shear-driven regulation matrix metalloproteinase (MMP)...
s t r y , N a ~~~n € Cancer Instltut~, ~e ~h e ~, ~a ~L n d 20892 and I ' T h ~o -~o v i -I ~t ~u fuer ~~o w ~s c P g Chemie I, Am b l L)-97074 W u ~~u Germany The pig human dihydropyrimidine dehydrogenase (DPD) cDNAs were cloned sequenced.m enzyme, expressed in Escherichia coli, catalyzed the reduction of uracil, thymine, 5-fluorouracil with kinetics approximating those published fox enzyme purified from mammalian liver.DPD could be significant quantities only when uracil was added to...