A5101817833- Bone health and treatments
- Ion channel regulation and function
- Heart Rate Variability and Autonomic Control
- Neuroscience and Neuropharmacology Research
- Neuroscience of respiration and sleep
- Cancer Cells and Metastasis
- Ubiquitin and proteasome pathways
- Wnt/β-catenin signaling in development and cancer
- Bone Metabolism and Diseases
- Receptor Mechanisms and Signaling
- Pain Mechanisms and Treatments
- Cancer Research and Treatments
- Cellular Mechanics and Interactions
- Ion Channels and Receptors
- Metabolism, Diabetes, and Cancer
- Cancer, Hypoxia, and Metabolism
- Neuropeptides and Animal Physiology
- Cardiac electrophysiology and arrhythmias
- Cancer, Stress, Anesthesia, and Immune Response
- Neuroendocrine regulation and behavior
- Neurotransmitter Receptor Influence on Behavior
- Pancreatic and Hepatic Oncology Research
- Berberine and alkaloids research
- Adipose Tissue and Metabolism
- Osteoarthritis Treatment and Mechanisms
Harbin Medical University
2016-2025
Ministry of Education of the People's Republic of China
2024-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
University of Indianapolis
2007-2023
Indiana University – Purdue University Indianapolis
2006-2023
Xinjiang Medical University
2011-2018
First Affiliated Hospital of Xinjiang Medical University
2011-2018
Donghua University
2017
University of Michigan–Dearborn
2017
Shandong Provincial QianFoShan Hospital
2012
(1) Background: Transient receptor potential vanilloid 3 (TRPV3) is a member of the TRP channels family Ca2+-permeant channels. The proteins some are highly expressed in cancer cells. This study aimed to assess clinical significance and biological functions TRPV3 non-small cell lung (NSCLC); (2) Methods: Immunohistochemistry was used detect expression NSCLC tissues adjacent noncancerous tissues. Western blot protein expressions TRPV3, CaMKII, p-CaMKII, CyclinA, CyclinD, CyclinE1, CDK2, CDK4,...
Abstract Osteocytes are mechanosensitive bone cells, but little is known about their effects on tumor cells in response to mechanical stimulation. We treated breast cancer with osteocyte-derived conditioned medium (CM) and fluid flow-treated (FFCM) 0.25 Pa 1 shear stress. Notably, CM FFCM at induced the mesenchymal-to-epithelial transition (MET), epithelial-to-mesenchymal (EMT). This suggested that of flow media depend intensity. Fluorescence resonance energy transfer (FRET)-based evaluation...
Background: Bone is a frequent site of metastases from breast cancer, but existing therapeutic options are not satisfactory. Although osteoblasts have active roles in cancer progression by assisting the vicious bone-destructive cycle, we employed counterintuitive approach activating pro-tumorigenic Wnt signaling and examined paradoxical possibility developing osteoblast-derived tumor-suppressive, bone-protective secretomes. Methods: was activated overexpression Lrp5 β-catenin as well...
Abstract While bone is a frequent target of breast cancer–associated metastasis, little known about the effects tumor–bone interactions on efficacy tumor-suppressing agents. Here we examined effect two FDA-approved dopamine modulators, fluphenazine and trifluoperazine, mammary tumor cells, osteoclasts, osteoblasts, osteocytes. These agents suppressed proliferation migration cells chiefly by antagonizing receptor D2 reduced resorption downregulating nuclear factor activated T cytoplasmic 1...
Background: Advanced breast cancer metastasizes to many organs including bone, but few effective treatments are available.Here we report that induced tumor-suppressing (iTS) MSCs protected bone from metastases while un-induced did not.Methods: iTS were generated by overexpressing Lrp5, β-catenin, Snail, or Akt.Their tumorsuppressing capability was tested using a mouse model of mammary tumors and metastasis, human tissues cell lines.Results: In model, the MSC-derived conditioned medium (MSC...
Rationale: Osteosarcoma (OS), a common malignant bone tumor, calls for the investigation of novel treatment strategies.Low-intensity vibration (LIV) presents itself as promising option, given its potential to enhance health and decrease cancer susceptibility.This research delves into effects LIV on OS cells mesenchymal stem (MSCs), with primary focus generating induced tumor-suppressing (iTSCs) tumor-suppressive conditioned medium (CM).Methods: To ascertain influence frequency, we employed...
MicroRNA (miRNA) and mitofusin-2 (Mfn2) are important in the development of cardiac hypertrophy, but target relationship mechanism associated with Ca2+ handling between SR mitochondria under hypertrophic condition is not established. Mfn2 expression, Mfn2-mediated interorganelle cross-talk, regulation by miRNA-20b (miR-20b) were evaluated using animal/cellular models state-of-the-art techniques. The results demonstrated that was downregulated miR-20b upregulated upon binding profile...
Abstract Osteocytes are the most abundant cells in bone, which is a frequent site of breast cancer metastasis. Here, we focused on Wnt signaling and evaluated tumor-osteocyte interactions. In animal experiments, mammary tumor were inoculated into fat pad tibia. The role Lrp5-mediated was examined by overexpressing silencing Lrp5 osteocytes establishing conditional knockout mouse model. results revealed that administration or their conditioned medium (CM) inhibited progression osteolysis....
Canines can identify prostate cancer with high accuracy by smelling volatile organic compounds (VOCs) in urine. Previous studies have identified VOC biomarkers for utilizing solid phase microextraction (SPME) gas chromatography–mass spectrometry (GC-MS) but not assessed the ability of VOCs to distinguish aggressive cancers. Additionally, previous investigations utilized murine models determined if results are translatable humans. To address these challenges, urine was collected from mice and...
We recently identified a myelinated vagal afferent subpopulation (Ah type) far more prevalent in female than male rats and showed that this difference extends to functionally specific visceral sensory afferents, baroreceptors of the aortic arch. Excitability Ah-type afferents is markedly reduced after ovariectomy (OVX). Here we tested hypothesis 17beta-estradiol can selectively restore excitability these sex-specific afferents. Acutely isolated neurons (VGN) from intact OVX adult were used...
Abstract Dopaminergic signaling plays a critical role in the nervous system, but little is known about its potential breast cancer and bone metabolism. A screening of ~1,000 biologically active compounds revealed that selective agonist dopamine receptor D1 (DRD1), A77636, inhibited proliferation 4T1.2 mammary tumor cells as well MDA-MB-231 cells. Herein, we examined effect A77636 on quality using mouse model metastasis from tumor. migration DRD1-dependent fashion suppressed development...
Delivery of bone marrow-derived mesenchymal stem cells (MSCs) to myocardium protects ischemic tissue through the paracrine release beneficial angiogenic and cytoprotective factors. Platelet-derived growth factor (PDGF)-BB, a potent mitogen MSCs, is involved in pathophysiology heart disease. However, role(s) PDGF MSC-mediated cardioprotection remains unknown. Here, we found that treatment MSCs resulted rapid activation both Akt ERK (central intracellular signal mediators), upregulated VEGF,...
Rationale:The progression of cancer cells depends on the soil and building an inhibitory might be a therapeutic option.We previously created tumor-suppressive secretomes by activating Wnt signaling in MSCs.Here, we examined whether anti-tumor can produced from tumor cells. Methods:Wnt was activated overexpressing β-catenin or administering BML284, activator.Their conditioned medium (CM) applied to tissues, effects CM were evaluated.Tumor growth mammary fat pad tibia C57BL/6 female mice also...
The brain is a common site of metastasis from advanced breast cancer but few effective treatments are available. We examined therapeutic option with conditioned medium (CM), focusing on the role Lrp5 and β-catenin in Wnt signaling, IL1ra osteocytes. Osteocytes presented innate anti-tumor effect overexpression above genes strengthened their action. In mouse model, injection CM inhibited mammary tumors tumor-driven osteolysis. Importantly, Lrp5- and/or IL1ra-overexpressing osteocytes or local...
Calsequestrins (Casqs), comprising the Casq1 and Casq2 isoforms, buffer Ca2+ regulate its release in sarcoplasmic reticulum of skeletal cardiac muscle, respectively. Human inherited diseases associated with mutations CASQ1 or CASQ2 include malignant hyperthermia/environmental heat stroke (MH/EHS) catecholaminergic polymorphic ventricular tachycardia. However, patients an MH/EHS event often experience arrhythmia for which underlying mechanism remains unknown.Working hearts from conventional...
Background: Advanced breast cancer frequently metastasizes to bone, but inhibiting tumor progression in chemotherapy may occasionally enhance tumorigenesis.Here, we employed a counterintuitive approach of overexpressing Yamanaka factors (Oct4, c-Myc, Sox2, and Klf4) examined conditioned medium (CM)-based treatment option with induced tumor-suppressing cells (iTSCs).Methods: In vitro proliferation migration assays were conducted using cell lines derived from cancer, as well prostate...
Background: During a developmental process, embryos employ varying tactics to remove unwanted cells.Using procedure analogous some of the embryonic cells, we generated tumor-eliminating conditioned medium (CM) from AMPK-inhibited lymphocytes and monocytes in peripheral blood mononuclear cells (PBMCs).Methods: AMPK signaling was inhibited by application pharmacological agent, Dorsomorphin, therapeutic effects their were evaluated using vitro cell cultures, ex vivo breast cancer tissues, mouse...
Nodose ganglia are composed of A-, Ah-and C-type neurons.Despite their important roles in regulating visceral afferent function, including cardiovascular, pulmonary, and gastrointestinal homeostasis, information about subtype-specific expression, molecular identity, function individual ion transporting proteins is scarce.Although experiments utilizing the sliced ganglion preparation have provided valuable insights into electrophysiological properties nodose neuron subtypes, detailed...
Abstract Cardiac hypertrophy is a compensatory response to mechanical stimuli and neurohormonal factors, ultimately progresses heart failure. The proteins of some transient receptor potential ( TRP ) channels, Ca 2+ ‐permeable nonselective cation channel, are highly expressed in cardiomyocytes, associated with the occurrence cardiac hypertrophy. Transient vanilloid 3 TRPV 3) member , however, functional role remains unclear. was elevated pathological hypertrophy, but not swimming...
Bone is a frequent site of metastasis from breast cancer, and desirable drug could suppress tumor growth as well metastasis‐linked bone loss. Currently, no able to cure cancer‐associated metastasis. In this study, we focused on statins that are known inhibit cholesterol production act antitumor agents. After an initial potency screening 7 U.S. Food Drug Administration‐approved statins, examined pitavastatin candidate for inhibiting tumor‐induced vitro analysis revealed acted inhibitor...
This study utilized a Förster resonance energy transfer (FRET)-based molecular tension sensor and live cell imaging to evaluate the effect of osteocytes, mechanosensitive bone cell, on migratory behavior tumor cells. Two lines derived from MDA-MB-231 breast cancer cells were transfected with vinculin quantitatively force in focal adhesions cell. Tumor treated MLO-A5 osteocyte-conditioned media (CM) decreased tensile forces their potential. exposed fluid flow-driven shear stress (FFCM)...
Despite the advance in medications past decade, aggressive breast cancer such as triple-negative is difficult to treat. Here, we examined a counter-intuitive approach converting human bone marrow-derived mesenchymal stem cells (MSCs) into induced tumor-suppressing by administering YS49, PI3K/Akt activator. Notably, PI3K-activated MSCs generated tumor-suppressive proteomes, while PI3K-inactivated tumor-promotive proteomes. In mouse model, daily administration of YS49-treated MSC-derived CM...