A5059830381- Cancer Research and Treatments
- Metabolomics and Mass Spectrometry Studies
- Photosynthetic Processes and Mechanisms
- Bladder and Urothelial Cancer Treatments
- Advanced Proteomics Techniques and Applications
- Amino Acid Enzymes and Metabolism
- Legume Nitrogen Fixing Symbiosis
- Ubiquitin and proteasome pathways
- Cancer Cells and Metastasis
- Veterinary Oncology Research
- Algal biology and biofuel production
- Glycosylation and Glycoproteins Research
- Agronomic Practices and Intercropping Systems
- Mitochondrial Function and Pathology
- Immunotherapy and Immune Responses
- Mass Spectrometry Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Mycorrhizal Fungi and Plant Interactions
- Cancer Genomics and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Microbial Inactivation Methods
- Cancer Immunotherapy and Biomarkers
- Adipose Tissue and Metabolism
- interferon and immune responses
- Protein Degradation and Inhibitors
Purdue University West Lafayette
2015-2025
State Street (United States)
2018-2025
Icahn School of Medicine at Mount Sinai
2024
Boston University
2024
Georgia State University
2024
Washington University in St. Louis
2024
Joint Center for Structural Genomics
2020
Pacific Northwest National Laboratory
2011-2014
Environmental Molecular Sciences Laboratory
2011-2013
Plant Biotechnology Institute
2008-2011
The objective of this study was to characterize the major proteomes and metabolites in beef exudate determine their relationship color oxidative quality muscles. Beef loin (LD) tenderloin (PM) muscles were cut into sections, individually vacuum-packaged, aged for 9, 16 23 days at 2 °C. Following aging, exudates collected analyzed both proteomics metabolomics profiles. Proteome analysis indicated clustering by muscle types, while profiling further clustered samples based on aging periods. PM...
Lipids are critical to brain structure and function, accounting for approximately 50% of its dry weight. However, the impact aging on lipids remains poorly characterized. To address this, here we applied three complementary mass spectrometry techniques: multiple reaction monitoring (MRM) profiling, untargeted liquid chromatography tandem (LC-MS/MS), desorption electrospray ionization-MS imaging (DESI-MSI). We used brains from mice age groups: adult (3–4 months), middle-aged (10 old (19–21...
Titanium dioxide metal oxide affinity chromatography (TiO(2)-MOAC) is widely regarded as being more selective than immobilized metal-ion (IMAC) for phosphopeptide enrichment. However, the widespread application of TiO(2)-MOAC to biological samples hampered by conflicting reports which experimental conditions are optimal. We have evaluated performance under a wide range loading and elution conditions. Loading stringent washing peptides with strongly acidic solutions ensured highly enrichment...
Protein glycosylation (e.g., N-linked glycosylation) is known to play an essential role in both cellular functions and secretory pathways; however, our knowledge of vivo N-glycosylated sites very limited for the majority fungal organisms including Aspergillus niger. Herein, we present first extensive mapping A. niger by applying optimized solid phase glycopeptide enrichment protocol using hydrazide-modified magnetic beads. The was initially mouse blood plasma secretome samples, it...
Abstract Global analyses of protein complex assembly, composition, and location are needed to fully understand how cells coordinate diverse metabolic, mechanical, developmental activities. The most common methods for proteome-wide analysis complexes rely on affinity purification-mass spectrometry or yeast two-hybrid approaches. These time consuming not suitable many plant species that refractory transformation genome-wide cloning open reading frames. Here, we describe the proof concept a...
Abstract Breast cancer brain metastases (BCBM) have a 5-20 year latency and account for 30% of mortality; however, mechanisms governing adaptation to the microenvironment remain poorly defined. We combine time-course RNA-sequencing BCBM development with Drosophila melanogaster genetic screen, identify Rab11b as functional mediator metastatic adaptation. Proteomic analysis reveals that controls cell surface proteome, recycling proteins required successful interaction microenvironment,...
Abstract Obesity is a growing epidemic worldwide and major risk factor for several chronic diseases, including diabetes, kidney disease, heart cancer. often leads to type 2 diabetes mellitus, via the increased production of proinflammatory cytokines such as tumor necrosis factor-α (TNFα). Our study combines different proteomic techniques investigate changes in global proteome, secretome phosphoproteome adipocytes under inflammation condition, well fundamental cross-talks between cellular...
Background: Advanced breast cancer metastasizes to many organs including bone, but few effective treatments are available.Here we report that induced tumor-suppressing (iTS) MSCs protected bone from metastases while un-induced did not.Methods: iTS were generated by overexpressing Lrp5, β-catenin, Snail, or Akt.Their tumorsuppressing capability was tested using a mouse model of mammary tumors and metastasis, human tissues cell lines.Results: In model, the MSC-derived conditioned medium (MSC...
Obesity caused by overnutrition is a major risk factor for non-alcoholic fatty liver disease (NAFLD). Several lipid intermediates such as acids, glycerophospholipids and sphingolipids are implicated in NAFLD, but detailed characterization of lipids their functional links to proteome phosphoproteome remain be elucidated. To characterize this complex molecular relationship, we used multi-omics approach conducting comparative proteomic, phoshoproteomic lipidomic analyses high fat (HFD) low...
Reversible protein phosphorylation is a key regulatory mechanism in cells. Identification and characterization of phosphoproteins requires specialized enrichment methods, due to the relatively low abundance these proteins, further complicated plants by high Rubisco green tissues. We present novel method for plant phosphoproteome analysis that depletes using polyethylene glycol fractionation utilizes immobilized metal-ion affinity chromatography enrich phosphoproteins. Subsequent separation...
The deubiquitinase (DUB) ubiquitin C-terminal hydrolase L1 (UCHL1) is expressed primarily in the central nervous system under normal physiological conditions. However, UCHL1 overexpressed various aggressive forms of cancer with strong evidence supporting as an oncogene lung, glioma, and blood cancers. In particular, level expression these cancers correlates increased invasiveness metastatic behavior, well poor patient prognosis. Although considered potential a therapeutic target, there...
Mammalian cells possess intrinsic mechanisms to prevent tumorigenesis upon deleterious mutations, including oncogene-induced senescence (OIS). The molecular underlying OIS are, however, complex and remain be fully characterized. In this study, we analyzed the changes in nuclear proteome phosphoproteome of human lung fibroblast IMR90 during progression induced by oncogenic RAS
Abstract Background Unicellular cyanobacteria of the genus Cyanothece are recognized for their ability to execute nitrogen (N 2 )-fixation in dark and photosynthesis light. An understanding these mechanistic processes an integrated systems context should provide insights into how might be optimized specialized environments and/or industrial purposes. Systems-wide dynamic proteomic profiling with mass spectrometry (MS) analysis reveal fundamental control regulation functions. Results To...
Information on the composition of protein complexes can accelerate mechanistic analyses cellular systems. Protein complex identifies genes that function together and provides clues about regulation within between pathways. Cytosolic control metabolic flux, signal transduction, abundance, activities cytoskeletal endomembrane It has been estimated one third all cytosolic proteins in leaves exist an oligomeric state, yet nearly remain unknown. Subunits stable copurify, combinations...
The plant endoplasmic reticulum-Golgi apparatus is the site of synthesis, assembly, and trafficking all noncellulosic polysaccharides, proteoglycans, proteins destined for cell wall. As grass species make walls distinct from those dicots noncommelinid monocots, it has been assumed that differences in cell-wall composition stem biosynthetic capacities their respective Golgi. However, immunosorbence-based screens carbohydrate linkage analysis polysaccharides Golgi membranes, enriched by...
Rationale:The progression of cancer cells depends on the soil and building an inhibitory might be a therapeutic option.We previously created tumor-suppressive secretomes by activating Wnt signaling in MSCs.Here, we examined whether anti-tumor can produced from tumor cells. Methods:Wnt was activated overexpressing β-catenin or administering BML284, activator.Their conditioned medium (CM) applied to tissues, effects CM were evaluated.Tumor growth mammary fat pad tibia C57BL/6 female mice also...
The brain is a common site of metastasis from advanced breast cancer but few effective treatments are available. We examined therapeutic option with conditioned medium (CM), focusing on the role Lrp5 and β-catenin in Wnt signaling, IL1ra osteocytes. Osteocytes presented innate anti-tumor effect overexpression above genes strengthened their action. In mouse model, injection CM inhibited mammary tumors tumor-driven osteolysis. Importantly, Lrp5- and/or IL1ra-overexpressing osteocytes or local...