A5062417379- Drug Transport and Resistance Mechanisms
- Amino Acid Enzymes and Metabolism
- Metabolism and Genetic Disorders
- Pharmacological Effects and Toxicity Studies
- Neuroscience and Neuropharmacology Research
- Barrier Structure and Function Studies
- Neonatal Health and Biochemistry
- Epilepsy research and treatment
- Molecular Sensors and Ion Detection
- Folate and B Vitamins Research
- Diet and metabolism studies
- Biochemical effects in animals
- Protein Interaction Studies and Fluorescence Analysis
- Advanced biosensing and bioanalysis techniques
- Adenosine and Purinergic Signaling
- Cholesterol and Lipid Metabolism
- Aldose Reductase and Taurine
- Polyamine Metabolism and Applications
- Trace Elements in Health
- Muscle metabolism and nutrition
- Synthesis and Biological Evaluation
- Nicotinic Acetylcholine Receptors Study
- Ion Transport and Channel Regulation
- HIV/AIDS drug development and treatment
- Biomedical Research and Pathophysiology
Teikyo University
2022-2024
University of Toyama
2014-2023
Tokushima University
2022
Tohoku University
2011-2012
Kanazawa University
2005-2010
University of Electro-Communications
2010
Augusta University
2010
Aoyama Gakuin University
2008
Osaka University
2000-2005
Nagahama Institute of Bio-Science and Technology
2005
Human cerebral microvascular endothelial cell line hCMEC/D3 is an established model of the human blood–brain barrier (BBB). The purpose present study was to determine, by means quantitative targeted absolute proteomics, protein expression levels in cells multiple transporters, receptors and junction proteins for comparison with our previously reported findings isolated brain microvessels. Among 91 target molecules, 12 2 receptors, 1 membrane marker were at quantifiable plasma fraction cells....
Abstract The purpose of this study was to clarify the expression Na + ‐dependent multivitamin transporter (SLC5A6/SMVT) and its contribution supply biotin pantothenic acid human brain via blood–brain barrier. DNA microarray immunohistochemical analyses confirmed that SLC5A6 is expressed in microvessels brain. absolute levels protein isolated monkey were 1.19 0.597 fmol/μg protein, respectively, as determined by a quantitative targeted proteomics technique. Using an antibody‐free method...
The organic cation/carnitine transporter OCTN2 is responsible for renal tubular reabsorption of its endogenous substrate, carnitine, although physiological role in small intestine remains controversial. Here we present direct evidence a predominant intestinal absorption carnitine based on experiments with juvenile visceral steatosis (<i>jvs</i>) mice, which have hereditary deficiency the <i>octn2</i> gene. Uptake assessed an Ussing-type chamber system, from apical surface was saturable and...
The purpose of this study was to construct and validate an in vitro three-dimensional blood-brain barrier (3DBBB) model system equipped with brain microvascular endothelial cells derived from human induced pluripotent stem (hiPS-BMECs).The 3D-BBB constructed by seeding hiPS-BMECs onto the capillary lane a MIMETAS OrganoPlate® 3-lane coated fibronectin/collagen IV. were incubated under continuous switchback flow OrganoFlow® for 2 days. 3D structure expression tight-junction proteins...
ABCA5 is a member of the ABC transporter A subfamily, and mouse orthologue (mABCA5) in newborn brain neural cells was identified by reverse transcription-PCR.Full-length cDNA cloning revealed that mABCA5 consists 1,642 amino acid residues its putative structure full-type having two sets six transmembrane segments nucleotide binding domain.Immunohistochemical studies expressed brain, lung, heart, thyroid gland.A subcellular localization analysis showed resident lysosomes late endosomes.Abca5...
In the present study, we attempted to identify membrane permeation process(es) primarily involved in molecular-weight-dependent biliary excretion of β-lactam antibiotics. A search literature indicated that molecular weight threshold operates mainly transport process across bile canalicular membranes. We confirmed clearance model biliary-excretion-type cephalosporin cefoperazone was reduced 10% control Eisai hyperbilirubinemic rats, which are genetically deficient multidrug...
Carnitine/organic cation transporter (OCTN1/SLC22A4) accepts various therapeutic agents as substrates in vitro and is expressed ubiquitously, although its function most organs has not yet been examined. The purpose of the present study was to evaluate functional expression OCTN1 small intestine liver, using octn1 gene knockout [octn1(-/-)] mice. After oral administration [(3)H]ergothioneine ([(3)H]ERGO), a typical substrate OCTN1, amount [(3)H]ERGO remaining intestinal lumen much higher...
Gastrointestinal (GI) absorption of certain therapeutic agents is thought to be mediated by solute carrier (SLC) transporters, although minimal in vivo evidence has been reported. Here, we show key roles postsynaptic density 95/disk-large/ZO-1 (PDZ) domain-containing protein, PDZK1, as a regulatory mechanism two carriers, Slc15a1 (oligopeptide transporter PEPT1) and Slc22a5 (carnitine/organic cation OCTN2) mouse small intestine using <i>pdzk1</i> gene knockout (<i>pdzk1</i><sup>–/–</sup>)...
H<sup>+</sup>/peptide transporter, PEPT1, is functionally expressed in some human cancer cell lines and might be a candidate molecular target for detection of cancers vivo using PET. The aim the present study was to establish novel tumor-imaging technology PET tracer targeted transporter(s). We also compared with <sup>18</sup>F-FDG, focusing on specificity their accumulation between tumor inflammatory tissues. <b>Methods:</b> A dipeptide tracer, <sup>11</sup>C-glycylsarcosine...
The purpose of this study was to determine absolute protein expression levels transporters at the porcine inner blood-retinal barrier (BRB) and compare transporter quantitatively among BRB, outer blood-brain (BBB), blood-cerebrospinal fluid (BCSFB). Crude membrane fractions isolated retinal capillaries (inner BRB) pigment epithelium (RPE, were prepared from eyeballs, while plasma brain (BBB) choroid plexus Protein 32 molecules, including 16 ATP-binding-cassette (ABC) 13 solute-carrier (SLC)...