A5078174999- Viral Infections and Outbreaks Research
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Hepatitis B Virus Studies
- Disaster Response and Management
- Virology and Viral Diseases
- Influenza Virus Research Studies
- Animal Disease Management and Epidemiology
- Hepatitis Viruses Studies and Epidemiology
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Hepatitis C virus research
- COVID-19 epidemiological studies
- Vaccine Coverage and Hesitancy
- Liver Disease Diagnosis and Treatment
- Vector-Borne Animal Diseases
- Cytomegalovirus and herpesvirus research
- Zoonotic diseases and public health
- Animal Virus Infections Studies
- Parvovirus B19 Infection Studies
- Immune responses and vaccinations
- Immunodeficiency and Autoimmune Disorders
- Bacillus and Francisella bacterial research
- COVID-19 diagnosis using AI
University of Lagos
2015-2025
Nigerian Institute of Medical Research
2020-2024
Lagos University Teaching Hospital
1999-2024
Federal Medical Centre
2020
Lagos State Health Service Commission
2020
Lagos State University
2018-2020
Federal University of Technology Owerri
2016
University of Ibadan
1988-1990
Lassa virus (LASV) causes a deadly haemorrhagic fever in humans, killing several thousand people West Africa annually. For 40 years, the Natal multimammate rat, Mastomys natalensis, has been assumed to be sole host of LASV. We found evidence that LASV is also hosted by other rodent species: African wood mouse Hylomyscus pamfi Nigeria, and Guinea erythroleucus both Nigeria Guinea. Virus strains from these animals were isolated BSL-4 laboratory fully sequenced. Phylogenetic analyses viral...
Introduction: success in curtailing the pandemic coronavirus disease (COVID-19) depends largely on a sound understanding of epidemiologic and clinical profile cases population as well case management approach. This study documents presenting characteristics, treatment modalities outcomes first 32 COVID-19 patients Nigeria.
In vitro studies have shown the efficacy of Ivermectin (IV) to inhibit SARS-CoV-2 viral replication, but questions remained as in-vivo applications. We set out explore and safety in persons infected with COVID19.We conducted a translational proof concept randomized, double blind placebo controlled, dose response parallel group study IV RT-polymerase chain reaction proven COVID 19 positive patients. Sixty-two patients were randomized three treatment groups. (A) 6 mg regime, (B) 12 regime...
Sub-Saharan Africa suffers from an excessively high endemicity of hepatitis B virus (HBV), but little is known about the prevalent genotypes. In this study, we investigated PreS1/PreS2/S genes 127 viruses obtained 12 locations in Mali, Burkina Faso, Togo, Benin, Nigeria, Cameroon, and Democratic Republic Congo. Except for those Cameroon HIV cohort (18/22 HBV genotype A), 96 105 sequences belonged to E (HBV/E), viral DNA was very similar (1.67% diversity) throughout vast HBV/E crescent, which...
One hundred and twenty-two new hepatitis B virus (HBV) preC/C sequences three complete genomes from major countries in West Africa were analysed. The majority of genotype E the only other found was A. Although for sequences, genetic diversity gene about two to times higher than that preS/S gene, it still considerably lower A sequences. HBV/E related most closely subgenotype D1 D2 Evidence recombination strains gene. Cameroon, Mali Nigeria could be divided phylogenetically into subtypes, A3...
This study describes an RT-PCR assay targeting the L RNA segment of arenaviruses. Conserved regions were identified in polymerase domain gene on basis published sequences for Lassa virus, lymphocytic choriomeningitis virus (LCMV), Pichinde and Tacaribe as well 15 novel LCMV, Ippy Mobala Mopeia determined this study. Using these target sites, a PCR detection all known Old World arenaviruses was developed optimized. The concentration that yields 95% positive results set replicate tests (95%...
The method of choice for the detection Lassa virus is reverse transcription (RT)-PCR. However, high degree genetic variability poses a problem with design RT-PCR assays that will reliably detect all strains. Recently, we encountered difficulties in detecting some strains from Liberia and Nigeria commonly used glycoprotein precursor (GPC) gene-specific assay (A. H. Demby, J. Chamberlain, D. W. Brown, C. S. Clegg, Clin. Microbiol. 32:2898-2903, 1994), which prompted us to revise protocol. new...
In 2014, Ebola virus (EBOV) was identified as the etiological agent of a large and still expanding outbreak disease (EVD) in West Africa much more confined EVD Middle Africa. Epidemiological evolutionary analyses confirmed that all cases both outbreaks are connected to single introduction each EBOV into human populations not directly connected. Coding-complete genomic sequence isolates revealed two were caused by novel variants, initial clinical observations suggest neither them should be...
ABSTRACT Hepatitis D virus (HDV) is a satellite of hepatitis B (HBV), and infection with this aggravates acute chronic liver disease. While HBV seroprevalence very high across sub-Saharan Africa, much less known about HDV in the region. In study, almost 2,300 blood serum samples from Burkina Faso ( n = 1,131), Nigeria 974), Chad 50), Central African Republic 118) were screened for HDV. Among 743 HBsAg-positive samples, 74 positive antibodies and/or RNA, considerable differences prevalence,...
ABSTRACT Recent Lassa virus strains from Nigeria were completely or partially sequenced. Phylogenetic analysis revealed the predominance of lineage II and III strains, existence a previously undescribed (sub)lineage in Nigeria, directional spread southern part country. The Bayesian also provided estimates for divergence times within clade.
Nigeria, with a population of over 190 million people, is rated among the 10 countries highest burden infectious and zoonotic diseases globally. In there exist sub-optimal surveillance system to monitor track priority zoonoses. We therefore conducted prioritization for first time in Nigeria guide prevention control efforts. Towards this, two-day in-country consultative meeting involving experts from human, animal, environmental health backgrounds prioritized using modified semi-quantitative...
Lagos state is the industrial nerve centre of Nigeria and was epicentre 2014 Ebola outbreak in as it now for current Coronavirus Disease (COVID-19) outbreak. This paper describes how lessons learned from informed emergency preparedness State ahead COVID-19 guided response.Following 2014, government provided governance by developing a policy on biosecurity oversight coordination strategies. Capacities response were strengthened training key staff, robust surveillance system, setting up...
Abstract Objectives To estimate the burden of Lassa fever in northern and central Edo, a state south Nigeria where has been reported. Methods Blood samples were obtained from 60 patients hospitalised at Irrua Specialist Teaching Hospital (ISTH), Irrua, with clinical suspicion 451 febrile outpatients seen ISTH hospitals Ekpoma, Iruekpen, Uromi, Auchi Igarra. All tested retrospectively by virus‐specific RT‐PCR. Outpatients additionally screened for antibodies indirect immunofluorescent...
Plasmodium falciparum-resistance to sulphadoxine-pyrimethamine (SP) has been largely reported among pregnant women. However, the profile of resistance markers SP dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) in general population are varied not frequently monitored. Currently, is used as partner drug for artemisinin combination therapy (SP-artesunate) some sub-Saharan African countries or a prophylactic intermittent preventive treatment malaria during pregnancy infants...
Sub-Saharan Africa is one of the regions globe with highest measles-related morbidity and mortality. Yet only seven virus isolates from this vast region have been phylogenetically characterized on basis their nucleoprotein, last in 1991. To characterize prevalent wild-type viruses to understand circulation pattern, a large panel (n = 45) was collected Ghana Nigeria 1997 1998. On nucleoprotein sequence, clearly belong clade B but reshuffling structure proposed, tentatively extending number...
Containment limited the 2014 Nigerian Ebola virus (EBOV) disease outbreak to 20 reported cases and 8 fatalities. We present here clinical data contact information for at least 19 case patients, full-length EBOV genome sequences 12 of 20. The detailed permits nearly complete reconstruction transmission tree outbreak. genomic are consistent with that tree. It confirms there was a single source infections, shows lineage nests within previously seen in Liberia but is genetically distinct from...
In an outbreak, effective detection of the aetiological agent(s) involved using molecular techniques is key to efficient diagnosis, early prevention and management spread. However, sequencing necessary for mutation monitoring tracking clusters transmission, development diagnostics vaccines drug development. Many methods are fast evolving reduce test turn-around-time increase through-put compared Sanger method; however, remains gold standard clinical research with its 99.99% accuracy This...
Diarrhoea disease is an important cause of morbidity and mortality in Nigerian children rotavirus has been identified as causative agent among below 5 years. We determined the prevalence rotavirus-induced diarrhoea under-5 s by electrophenotyping. Stool samples were collected from eligible subjects who presented with acute diarrhoea. The processed for viral studies Among 299 recruited, 55.9% positive rotavirus. Eighty percent recruited patients 24 months. There was a significant decrease...
ABSTRACT Introduction In vitro studies have shown the efficacy of Ivermectin (IV) to inhibit SARS - CoV-2 viral replication, but questions remained as In-vivo applications. We set out explore and safety in persons infected with COVID19. Methods conducted a translational proof concept (PoC) randomized, double blind placebo controlled, dose response, parallel group study IV RT PCR proven COVID 19 positive patients. 62 patients were randomized 3 treatment groups. (A) 6mg regime, (B)IV 12 mg...