A5033108538- Lysosomal Storage Disorders Research
- Calcium signaling and nucleotide metabolism
- 3D Printing in Biomedical Research
- Autophagy in Disease and Therapy
- Alzheimer's disease research and treatments
- Pregnancy and preeclampsia studies
- Electrospun Nanofibers in Biomedical Applications
- Erythrocyte Function and Pathophysiology
- Regulation of Appetite and Obesity
- Birth, Development, and Health
- Cellular transport and secretion
- Wound Healing and Treatments
- Pancreatic function and diabetes
- Cellular and Composite Structures
- Aluminum toxicity and tolerance in plants and animals
- Seaweed-derived Bioactive Compounds
- Breast Implant and Reconstruction
- Curcumin's Biomedical Applications
- Prion Diseases and Protein Misfolding
- Sarcoma Diagnosis and Treatment
- Cholesterol and Lipid Metabolism
- PARP inhibition in cancer therapy
- Protein Kinase Regulation and GTPase Signaling
- Adenosine and Purinergic Signaling
- Metabolism, Diabetes, and Cancer
San Sebastián University
2021-2024
Pontificia Universidad Católica de Chile
2015-2021
Universidad de Santiago de Chile
2008-2020
University of South Carolina
2017-2019
University of Concepción
2010-2014
The University of Texas at El Paso
2014
Chronic wounds such as diabetic foot ulcers and venous leg are common problems in people suffering from type 2 diabetes. These can cause pain, nerve damage, eventually leading to or amputation. types of very difficult treat sometimes take months even years heal because many possible complications during the process. Allogeneic skin grafting has been used improve wound healing, but majority grafts do not survive several days after being implanted. We have studying behavior fibroblasts...
Resveratrol (RSV) and nicotinamide (NAM) have garnered considerable attention due to their anti-inflammatory anti-aging properties. NAM is a transient inhibitor of class III histone deacetylase SIRTs (silent mating type information regulation 2 homologs) SIRT1 an poly-ADP-ribose polymerase-1 (PARP1). The debate on the relationship between RSV has precluded use as therapeutic drug. Recent work demonstrated that facilitates tyrosyl-tRNA synthetase (TyrRS)-dependent activation PARP1. Moreover,...
Abstract Preeclampsia (PE) poses a considerable risk to the long‐term cardiovascular health of both mothers and their offspring due hypoxic environment in placenta leading reduced fetal oxygen supply. Cholesterol is vital for development by influencing placental function. Recent findings suggest an association between hypoxia, disturbed cholesterol homeostasis, PE. This study investigates influence hypoxia on homeostasis. Using primary human trophoblast cells placentae from women with PE,...
Purpose The purpose of this paper is to present results an investigation, where the elastic tensor based on engineering constants sinterized Nylon 12 characterized and modeled considering a transversely isotropic behavior as function apparent density (relative mass density). Design/methodology/approach ultrasound propagation velocity measurement through material in specific directions by means pulse transmission method was used, relating elements phase magnitude Christoffel's equation. In...
It has recently been proposed that hypothalamic glial cells sense glucose levels and release lactate as a signal to activate adjacent neurons. GK (glucokinase), the hexokinase involved in sensing pancreatic β-cells, is also expressed hypothalamus. However, it not clearly determined if and/or neuronal express this protein. Interestingly, tanycytes, glia cover ventricular walls of hypothalamus, are contact with CSF (cerebrospinal fluid), capillaries arcuate nucleus neurons; would be expected...
Osteosarcoma is the most common type of malignant bone cancer, accounting for 35% primary malignancies. Because cancer cells utilize glucose as their energy substrate, expression and regulation transporters (GLUT) may be important in tumor development progression. GLUT has not been studied previously human osteosarcoma cell lines. Furthermore, although insulin insulin-like growth factor (IGF-I) play an role proliferation progression, these hormones on uptake, possible relation to...
Niemann–Pick type C disease (NPCD) is a lysosomal storage (LSD) characterized by abnormal cholesterol accumulation in lysosomes, impaired autophagy flux, and dysfunction. The activation of transcription factor EB (TFEB), master function regulator, reduces the substrates LSDs where degradative capacity cells compromised. Genistein can pass blood–brain barrier activate TFEB. Hence, we investigated effect TFEB genistein toward correcting NPC phenotype. We show that promotes translocation to...
Glucokinase (GK), the hexokinase involved in glucose sensing pancreatic β cells, is also expressed hypothalamic tanycytes, which cover ventricular walls of basal hypothalamus and are implicated an indirect control neuronal activity by glucose. Previously, we demonstrated that GK was preferentially localized tanycyte nuclei euglycemic rats, has been reported hepatocytes suggestive presence regulatory protein, GKRP. In present study, intracellular localization hepatic tissues same rats under...
One of the pathological hallmarks Alzheimer's disease (AD) is presence amyloid plaques, which are deposits misfolded and aggregated amyloid-beta peptide (Aβ). The role c-Abl tyrosine kinase in Aβ-mediated neurodegeneration has been previously reported. Here, we investigated therapeutic potential inhibiting using imatinib. We developed a novel method, based on technique used to detect prions (PMCA), measure minute amounts misfolded-Aβ blood AD transgenic mice. found that imatinib reduces...
Niemann-Pick type A (NPA) disease is a fatal lysosomal neurodegenerative disorder caused by the deficiency in acid sphingomyelinase (ASM) activity. NPA patients present severe and progressive neurodegeneration starting at an early age. Currently, there no effective treatment for this die between 2 3 years of characterized accumulation sphingomyelin lysosomes dysfunction autophagy-lysosomal pathway. Recent studies show that c-Abl tyrosine kinase activity downregulates autophagy Interestingly,...
Targeted therapies have changed the treatment of cancer, giving new hope to many patients in recent years. The shortcomings targeted including acquired resistance, limited susceptible patients, high cost, and toxicities, led necessity combining these with other or chemotherapeutic treatments. Natural products are uniquely capable synergizing non-targeted anticancer regimens due their ability affect multiple cellular pathways simultaneously. Compounds which provide an additive effect often...
The current engineered skin substitutes for diabetic foot ulcer treatment lack effective host integration. goal of this research is to create a wound care material that promotes integration with tissue. We have been investigating printable biodegradable scaffold composed gelatin and oxidized alginate, both materials very high biocompatibility low toxicity. investigated the printability alginate its use as an ‘ink’ drop-on-demand crosslinking gelatin. was characterized by...
We present two studies that assessed vascularization of implanted bioprinted grafts. The first study involves vascular networks build into skin A modified inkjet printer allowed depositing human microvascular endothelial cells fibrin networked channels. Neonatal dermal fibroblast and neonatal epidermal keratinocytes were manually mixed a collagen matrix, which sandwiched the networks. full thickness wound was created at top back athymic nude mice graft covered this wound. As control,...