Summary Terminal restriction fragment length polymorphism (T‐RFLP) analysis of PCR‐amplified nitrogenase gene ( nifH ) fragments is a rapid technique for profiling diazotrophic microbial communities without the necessity cultures study. Here, we examined impact N‐fertilization, plant genotype and environmental conditions on populations in association with roots rice Oryza species) by T‐RFLP community found marked effects composition community. We change population structure within 15 days...

Accumulating evidence has indicated that the multiple drug resistant Vibrio parahaemolyticus may pose a serious threat to public health and economic concerns for humans globally. Here, two lytic bacteriophages, namely vB_VpS_BA3 vB_VpS_CA8, were isolated from sewage collected in Guangzhou, China. Electron microscopy studies revealed both virions taxonomically belonged Siphoviridae family with icosahedral head long non-contractile tail. The double-stranded DNA genome of phage BA3 was composed...

Fifteen bacterial strains isolated from molasses grass ( Melinis minutiflora Beauv.) were identified as nitrogen-fixers by using the acetylene-reduction assay and PCR amplification of nifH gene fragments. These classified a unique group insertion sequence-PCR fingerprinting, SDS-PAGE protein patterns, DNA–DNA hybridization, 16S rRNA sequencing morphological characterization. Phylogenetic analysis indicated that these diazotrophic belonged to genus Azospirillum closely related lipoferum (with...

Nodulation is an essential process for biological nitrogen (N2) fixation in legumes, but its regulation remains poorly understood. Here, a β-expansin gene, GmEXPB2, was found to be critical soybean (Glycine max) nodulation. GmEXPB2 preferentially expressed at the early stage of nodule development. β-Glucuronidase staining further showed that mainly localized vascular trace and bundles, as well cortical parenchyma cells, suggesting might involved cell wall modification extension during...

Using a combination of phenotypic screening, genetic and statistical analyses, high-throughput genome-wide sequencing, we have finely mapped dominant Phytophthora resistance gene in soybean cultivar Wayao. root rot (PRR) caused by sojae is one the most important soil-borne diseases many soybean-production regions world. Identification resistant gene(s) incorporating them into elite varieties are an effective way for breeding to prevent from being harmed this disease. Two populations 191 F2...

Abstract Perovskite light‐emitting diodes (PeLEDs) have demonstrated significant potential in the display sector, attributed to their wide color gamut, narrow emission spectra, and cost‐effectiveness. Despite rapid advancements red green PeLEDs, attainment of high brightness external quantum efficiency (EQE) for blue PeLEDs remains a considerable challenge, which substantially limits practical applications white lighting optical communication. In this study, method passivation quasi‐2D...

ABSTRACT In addition to forming symbiotic nodules on legumes, rhizobial strains are members of soil or rhizosphere communities occur as endophytes, e.g., in rice. Two which have been isolated from root the aquatic legumes Aeschynomene fluminensis (IRBG271) and Sesbania aculeata (IRBG74) were previously found promote rice growth. analyzing their phylogenetic positions, we assessed suitability 16S-23S ribosomal DNA (rDNA) intergenic spacer (IGS) sequences for differentiation closely related...

Forty-eight rhizobial isolates from root nodules of Indigofera and Kummerowia, two genera annual or perennial wild legumes growing in the Loess Plateau north-western China, were characterized by a polyphasic approach. Two main groups, cluster 1 2, defined based upon results numerical taxonomy, SDS-PAGE whole-cell proteins DNA relatedness. All within isolated they identified as Rhizobium strains 16S rRNA gene analysis. relatedness 29.5-48.9% was obtained among reference for species. Cluster 2...

Ninety-five rhizobial strains isolated from Astragalus adsurgens growing in the northern regions of China were classified into three main groups, candidate species I, II and III, based on a polyphasic approach. Comparative analysis full-length 16S rRNA gene sequences representative showed that I Mesorhizobium , while which consisted non-nodulating strains, was closely related to Agrobacterium tumefaciens . The phylogenetic relationships some also confirmed by sequencing glnA genes, used as...

During a study of endophytic nitrogen-fixing bacteria present in the wild rice species Oryza alta, eight novel isolates were obtained from surface-sterilized roots and classified genus Rhizobium on basis almost-complete 16S rRNA gene sequence analysis. These strains can nodulate Phaseolus vulgaris Glycine max. The highly similar protein patterns, DNA fingerprint patterns insertion sequence-based PCR (IS-PCR) DNA–DNA hybridizations showed that these members same species. closest phylogenetic...

Twelve facultatively anaerobic, endophytic diazotrophs were isolated from surface-sterilized roots of the wild rice species Oryza latifolia and characterized by phenotypic molecular methods. Six isolates grouped together as group A characters, this grouping was confirmed SDS-PAGE whole-cell protein patterns insertion sequence-based PCR (IS-PCR) Phylogenetic analysis 16S rRNA gene sequence indicated that A, represented strain Ola 51T, is closely related to Enterobacter radicincitans D5/23T...

One of the overarching goals soybean breeding is to develop lines that combine increased yield with improved quality characteristics. High-density-marker QTL mapping can serve as an effective strategy identify novel genomic information facilitate crop improvement. In this study, we genotyped a recombinant inbred line (RIL) population (Zhonghuang 24 × Huaxia 3) using restriction-site associated DNA sequencing (RAD-seq) approach. A high-density genetic map was constructed and used several QTLs...

Twenty-nine rhizobial isolates from root nodules of Astragalus and Lespedeza spp. growing in the Loess Plateau China were characterized by numerical taxonomy, RFLP sequencing PCR-amplified 16S rRNA genes, measurement DNA G+C content, DNA–DNA relatedness cross-nodulation with selected legume species. Based on results formed two clusters (1 2) some single at a similarity level 82 %. Cluster 1 contained six 2 consisted nine was greater than 80 % among within cluster 2. Phylogenetic analysis...

Twelve facultatively anaerobic, endophytic diazotrophs were isolated from surface-sterilized roots of the wild rice species Oryza latifolia and characterized by phenotypic molecular methods. Six isolates grouped together as group A characters, this grouping was confirmed SDS-PAGE whole-cell protein patterns insertion sequence-based PCR (IS-PCR) Phylogenetic analysis 16S rRNA gene sequence indicated that A, represented strain Ola 51(T), is closely related to Enterobacter radicincitans...

Eight fast-growing rhizobial isolates from Xinjiang soils were identified as Sinorhizobium xinjiangense by analyses of 16S rRNA gene sequences, SDS-PAGE proteins, intergenic spacer sequences and DNA-DNA hybridization. Based on all the results, these reference strains for S. a distinct genomic species, although genes closely related to that fredii.

Sinorhizobium morelense sp. nov. is described to designate a group of bacteria isolated from root nodules Leucaena leucocephala. S. shows 98% 16S rRNA gene sequence similarity some species and Ensifer adhaerens. This novel distinguished other E. adhaerens by DNA-DNA hybridization, 165 restriction fragments distinctive phenotypic features. Strains this are highly resistant antibiotics, such as carbenicillin (1 mg ml(-1)), kanamycin (500 microg ml(-1)) erythromycin (300 ml(-1)). They do not...

Three aniline-degrading bacteria, strains DN316(T), DN316-1 and DN365, were isolated from activated sludge. According to 16S rRNA gene sequence-based phylogenetic analysis, the isolates belonged genus Rhizobium, with Rhizobium ( = Agrobacterium) radiobacter LMG 140(T) as closest relative, 96.5 % sequence similarity. Phylogenetic analysis of representative strain DN316(T) using sequences glnA, thrC recA genes 16S-23S intergenic spacer region confirmed arrangement obtained gene. DNA-DNA...

One of the most concerning global environmental issues is pollution agricultural soils by heavy metals (HMs), especially cadmium, which not only affects human health through Cd-containing foods but also impacts quality rice. The soil’s nitrification and denitrification processes, coupled with release volatile organic compounds plants, raise substantial concerns. In this review, we summarize recent literature related to deleterious effects Cd on both soil processes N cycle rice quality,...