A5039806950- Oral microbiology and periodontitis research
- Antimicrobial Peptides and Activities
- Oral Health Pathology and Treatment
- Salivary Gland Disorders and Functions
- Dental Health and Care Utilization
- Fatty Acid Research and Health
- Gut microbiota and health
- Infective Endocarditis Diagnosis and Management
- Dental Erosion and Treatment
- Bacterial biofilms and quorum sensing
- Probiotics and Fermented Foods
- Rheumatoid Arthritis Research and Therapies
- Bacterial Identification and Susceptibility Testing
- Streptococcal Infections and Treatments
- Bone and Dental Protein Studies
- Endodontics and Root Canal Treatments
- Immune Response and Inflammation
- dental development and anomalies
- Antibiotic Resistance in Bacteria
- Oral and gingival health research
- HIV/AIDS oral health manifestations
- Dental Research and COVID-19
- Breastfeeding Practices and Influences
- Diet and metabolism studies
- Neonatal Health and Biochemistry
University of Leeds
2015-2024
Forsyth Technical Community College
2022
Temple College
2022
Temple University
2022
Wellcome Trust
2015-2021
St James's University Hospital
2015-2021
Oregon Health & Science University
2018
Leeds Dental Hospital
2001-2016
Tufts Medical Center
2006-2009
The Honourable Society of Lincoln's Inn
2000
Objectives: The purpose of this study was to evaluate the clinical plaque disclosing agent erythrosine as a photosensitizer in photodynamic killing oral bacterium Streptococcus mutans grown biofilm. Methods: S. biofilms 200 μm thickness were constant-depth film fermenter. In addition determining localization within using confocal laser scanning microscopy (CLSM), we compared bacterial efficacy with that two well-characterized photosensitizers, methylene blue (MB) and photofrin. Incubations...
ABSTRACT Streptococcus salivarius is an early colonizer of human oral and nasopharyngeal epithelia, strain K12 has reported probiotic effects. An emerging paradigm indicates that commensal bacteria downregulate immune responses through the action on NF-κB signaling pathways, but additional mechanisms underlying actions are not well understood. Our objective here was to identify host genes specifically targeted by comparing their with elicited pathogens determine if S. modulates epithelial...
The lytic peptides, cecropins, were originally isolated from the haemolymph of giant silk moth, Hyalophora cecropia and possess antibacterial anticancer activity in vitro. This study investigated antimicrobial these peptides against human pathogens using standardised assay techniques, cecropin B on outer inner bacterial membranes. From a panel 15 organisms, Gram-negative bacteria generally more sensitive to cecropins than Gram-positive especially lipopolysaccharide defective mutant,...
Immunomodulatory commensal bacteria are proposed to be essential for maintaining healthy tissues, having multiple roles including priming immune responses ensure rapid and efficient defences against pathogens. The default state of oral like the gut, is one inflammation which may balanced by regulatory mechanisms activities anti-inflammatory resident that modulate Toll-like receptor (TLR) signalling or NF-κB activation, influence development cells. However, widespread ability normal organisms...
The prevalence of periodontitis is increased in patients with rheumatoid arthritis (RA) and periodontopathic bacteria can citrullinate proteins. Periodontitis may, therefore, be an initiator RA a target for prevention. Periodontal disease periodontal have not been investigated at-risk individuals autoimmunity but no arthritis.To examine anti-cyclic citrullinated protein (anti-CCP) antibody-positive without arthritis.This cross-sectional study took place at teaching hospital from April 27,...
Abstract Periodontitis is associated with shifts in the balance of subgingival microbiome. Many species that predominate disease have not been isolated from healthy sites, raising questions as to origin these putative pathogens. The study aim was determine whether periodontal pathogens could be enriched pooled saliva, plaque and tongue samples dentally-healthy adult volunteers using growth media simulate nutritional aspects inflamed environment. microbiome characterised before after...
An increased prevalence of periodontitis and perturbation the oral microbiome has been identified in patients with rheumatoid arthritis (RA). The periodontal pathogen Porphyromonas gingivalis may cause local citrullination proteins, potentially triggering anti-citrullinated protein antibody production. However, it is not known if dysbiosis precedes onset clinical arthritis. This study comprehensively characterised anti-cyclic citrullinated peptide (anti-CCP) positive at-risk individuals...
T he occurrence and surface properties of Prevotella intermedia P. nigrescens in healthy sites periodontic endodontic infections were studied among 73 strains, tentatively identified as . Fifteen strains from necrotic root canal infections, 41 periodontal samples, 17 isolates obtained gingival sites. Identification either or was based on differences malate glutamate dehydrogenase electrophoretic mobilities which allowed unambiguous separation The majority samples (29 strains). In only 4 out...
Caries occurs at inaccessible stagnation sites where plaque removal is difficult. Here, the penetration through of protective components, such as fluoride, likely to be crucial in caries inhibition. We hypothesized that topically applied fluoride would readily penetrate deposits. In this study, biofilms generated vivo on natural enamel surfaces were exposed NaF (1000 ppm F − ) for 30 or 120 sec (equivalent toothbrushing) min. Biofilms then sectioned throughout their depth, and content each...
There has been a paradigm shift towards an ecological and microbial community-based approach to understanding oral diseases. This significant implications for approaches therapy raised the possibility of developing novel strategies through manipulation resident microbiota modulation host immune responses. The increased popularity using probiotic bacteria and/or prebiotic supplements improve gastrointestinal health prompted interest in utility this applications. Evidence now suggests that...
The aim of this study was to determine if cocoa polyphenols could interfere with biofilm formation by Streptococcus mutans or sanguinis, and reduce acid production from sucrose S. mutans. antimicrobial activity assessed against cariogenic (S. mutans) health-associated sanguinis) species minimum inhibitory concentration assays. Cocoa polyphenol dimer, tetramer, pentamer inhibited the growth whereas unaffected. However, pretreatment surfaces (35 microM) reduced at 4 24 h, effects on sanguinis...