A5058192339- Inflammatory Bowel Disease
- Helicobacter pylori-related gastroenterology studies
- Microscopic Colitis
- Adipokines, Inflammation, and Metabolic Diseases
- Cytokine Signaling Pathways and Interactions
- Reproductive System and Pregnancy
- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Psoriasis: Treatment and Pathogenesis
- Extracellular vesicles in disease
- Inflammasome and immune disorders
- Biosimilars and Bioanalytical Methods
- Nuclear Structure and Function
- Ion Channels and Receptors
- Immunodeficiency and Autoimmune Disorders
- Gastrointestinal motility and disorders
- Eosinophilic Esophagitis
- interferon and immune responses
- Lipid metabolism and disorders
- Caveolin-1 and cellular processes
- Biological and pharmacological studies of plants
- Immune Response and Inflammation
- Apelin-related biomedical research
- Ginseng Biological Effects and Applications
- Clusterin in disease pathology
Charité - Universitätsmedizin Berlin
2018-2025
Humboldt-Universität zu Berlin
2018-2025
Freie Universität Berlin
2018-2025
MSB Medical School Berlin
2022
Franklin University
2021
University of Milan
2017-2020
King's College London
2018-2020
Guy's Hospital
2020
Medical Research Council
2014-2018
Sana Klinikum Lichtenberg
2018
Abstract Regulatory T cells (Treg) are a subpopulation of that maintain tolerance to self and limit other immune responses. They achieve this through different mechanisms including the release extracellular vesicles (EVs) such as exosomes shown by us, others. One ways Treg derived EVs inhibit target effector is via transfer miRNA. Another key for immunoregulatory function Tregs dendritic (DCs). In study we demonstrate directly, first time, miRNAs transferred from DCs EVs. particular two...
Exposure to particulate matter (PM) is associated with increased incidence of cardiovascular disease and coagulation, but the molecular mechanisms underlying these associations remain unknown. Obesity may increase susceptibility adverse effects PM exposure, exacerbating on diseases. Extracellular vesicles (EVs), which travel in body fluids transfer microRNAs (miRNAs) between tissues, might play an important role PM-induced risk. We sought determine whether levels aerodynamic diameter ≤ 10 μm...
Regulatory T cells (Tregs) are a subpopulation of CD4+ with fundamental role in maintaining immune homeostasis and inhibiting unwanted responses using several different mechanisms. Recently, the intracellular transfer molecules between Tregs their target has been shown via trogocytosis release small extracellular vesicles (EVs). In this study, CD4+CD25+CD127lo human were found to produce EVs capable proliferation effector (Teffs) dose dependent manner. These also modified cytokine profile...
Article7 May 2020Open Access Homeostatic and pathogenic roles of GM3 ganglioside molecular species in TLR4 signaling obesity Hirotaka Kanoh Division Glycopathology, Institute Molecular Biomembrane Glycobiology, Tohoku Medical Pharmaceutical University, Sendai, Japan Search for more papers by this author Takahiro Nitta Shinji Go Kei-ichiro Inamori Lucas Veillon Wataru Nihei Mayu Fujii Department Chemistry, Graduate School Science, Osaka Osaka, Kazuya Kabayama Atsushi Shimoyama Koichi Fukase...
Abstract Leptin has been shown to modulate intestinal inflammation in mice. However, clinical evidence regarding its immune-stimulatory potential human Crohn’s disease remains sparse. We here describe a patient with the unique combination of acquired generalized lipodystrophy and (AGLCD) featuring lack adipose tissue, leptin deficiency inflammation. Using mass flow cytometry, immunohistochemistry functional metabolic analyses, AGLCD was compared healthy individuals patients immune cell...
Abstract Background Crohn’s disease (CD) is associated with creeping fat, characterized by hyperplasia of mesenteric adipocytes and increased secretion adipokines, including leptin (1). However, limited models currently exist to study the effect adipose tissue on intestinal inflammation. Therefore, acquired generalized lipodystrophy (AGLCD), a rare metabolic disorder complete loss fat inflammation, could provide unique model further elucidate contribution pathogenesis inflammatory bowel...
Here we provide compelling evidence that inflammation in a murine model of Crohn’s disease–like is characterized by an immune reaction presumably directed at disease-relevant microflora genetically susceptible host with impaired mucosal barrier function and bacterial clearance.
Acquired generalized lipodystrophy (AGL) is a rare metabolic disorder frequently associated with autoimmunity. Its etiology incompletely understood and the impact of adipose tissue loss on autoimmunity intestinal inflammation in AGL remains unclear. Using mass cytometry single-cell RNA sequencing, we observed an oligoclonal expansion T cells periphery inflamed intestine patient Crohns disease (AGLCD). To explore if triggers lymphoproliferation, studied lipodystrophic mice as model for AGL....
Abstract The IL-36 signaling pathway has recently been identified as a key regulator of intestinal homeostasis and inflammation. However, the role mutations in IL-36R pathogenesis inflammatory bowel disease remains unclear. We here four Crohn’s patients with heterozygous missense receptor antagonist ( IL36RN , IL-36RA). Experimental overexpression functional assays demonstrated that two resulted reduced expression IL-36RA. In-depth immune profiling one -mutated patient revealed an increased...
Three new phenanthrene derivatives (1, 2, 4), one fluorenone (3), and four known compounds (5–8) were isolated from the ethyl acetate extract of Dendrobium crumenatum Sw. stems using column chromatography. The chemical structures elucidated by analysis spectroscopic data. absolute configuration 4 was determined electronic circular dichroism calculation. We also evaluated immunomodulatory effects D. in human peripheral blood mononuclear cells healthy individuals those patients with multiple...
Intestinal epithelial barrier function in celiac disease (CeD) patients is altered. However, the mechanism underlying this effect not fully understood. The aim of current study was to evaluate role monocytes eliciting defect CeD. For purpose, human were isolated from peripheral blood mononuclear cells (PBMCs) active and inactive CeD healthy controls. PBMCs sorted for expression CD14 co-cultured with intestinal (IECs, Caco2BBe). Barrier function, as well tight junctional alterations,...
Abstract Background The interleukin 36 (IL-36) family includes the agonists IL-36α, IL-36β, IL-36γ, which activate NF-κB pathway and natural antagonist IL-36RA, inhibits IL-36 signaling. While missense mutations in gene encoding IL-36RA (IL36RN) are associated with severe skin inflammation, their role Crohn’s disease (CD) is currently unknown. Therefore, we here investigated if IL36RN contribute to inflammation CD whether signaling might represent a potential drug target for personalized...
Abstract Background Crohn’s disease (CD) is associated with creeping fat, characterized by hyperplasia of mesenteric adipocytes and increased secretion various adipokines, including leptin. Leptin can promote intestinal inflammation inducing pro-inflammatory cytokines such as TNFα in murine human lymphocytes. Recent meta-analyses have shown that body mass index (BMI) increases the risk developing CD. However, whether surgical removal fat could improve outcome CD patients impact fat-derived...
Abstract Background Inflammatory bowel disease (IBD) represents a major clinical challenge in need of new treatment modalities to improve patient care. Store-operated calcium entry (SOCE) is the predominant influx pathway T cells, regulating many their functional properties. However, it currently unknown whether pharmacologic inhibition SOCE suitable drug target IBD. Therefore, we aimed investigate effects inhibitors (SOCEi) on lymphocytes and epithelial cells isolated from IBD patients....
Abstract Objective Inflammatory bowel disease (IBD) is characterized by dysregulated intestinal immune responses and constitutes a major clinical challenge in need of new treatment modalities to improve patient care. Store-operated Ca 2+ entry (SOCE) the predominant influx pathway T cells other cells, regulating many their functional properties. It currently unknown whether pharmacologic blockade SOCE represents suitable drug-target for IBD treatment. Design Using mass flow cytometry effects...
Abstract Background The interleukin 36 (IL-36) family consists of three agonists (IL-36α, IL-36β, IL-36γ), that activate the NF-κB pathway and lead to production pro-inflammatory cytokines natural antagonist (IL-36RA), which inhibits IL-36 signaling. While it is known signaling important in skin, has been only recently reported might play an role maintaining gut homeostasis development fibrosis (Scheibe et al., 2019). Here, we aim understand how IL-36RA mutations contribute maintenance...
Abstract Background Crohn’s disease (CD) is characterized by chronic inflammation that can discontinuously affect any segment of the gastrointestinal tract. New findings concerning clinical behaviour, epidemiology, genetics, and gut microbiota suggest CD in ileum (iCD) colon (cCD) should be considered as two distinct subtypes IBD. As systemic immune cell signatures differ between UC, we propose a similar strategy for differentiation iCD cCD. The aim this study to analyse compare peripheral...
Inflammatory bowel disease (IBD) is caused by complex interactions of dysregulated immune responses, defects in the intestinal epithelial barrier and translocating microbiota. IBD includes Crohn’s (CD) ulcerative colitis. CD characterised presence creeping fat. Although fat positively correlates with transmural inflammation, its functional nature largely unknown. The most used experimental model for employs dextran sodium sulfate (DSS) to induce damage thus colon inflammation. cell...