A5083008449- Banana Cultivation and Research
- Plant Gene Expression Analysis
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Sphingolipid Metabolism and Signaling
- Postharvest Quality and Shelf Life Management
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Lipid Membrane Structure and Behavior
- Plant Stress Responses and Tolerance
- Lysosomal Storage Disorders Research
- Plant Pathogenic Bacteria Studies
- Photosynthetic Processes and Mechanisms
- Microbial Metabolism and Applications
- Polysaccharides and Plant Cell Walls
- Phytochemicals and Antioxidant Activities
- Fungal Biology and Applications
- Plant Reproductive Biology
- Plant Surface Properties and Treatments
- Plant biochemistry and biosynthesis
- Plant nutrient uptake and metabolism
- Mycorrhizal Fungi and Plant Interactions
- Phytoplasmas and Hemiptera pathogens
Key Laboratory of Guangdong Province
2023-2025
Guangdong Academy of Agricultural Sciences
2016-2025
Ministry of Agriculture and Rural Affairs
2019-2025
Sun Yat-sen University
2010-2020
Institute of Fruit Tree and Tea Science
2020
Stellenbosch University
2019
Agricultural Research Organization
2014-2016
Single-walled carbon nanotubes (SWCNTs) have many unique structural and mechanical properties. Their potential applications, especially in biomedical engineering medical chemistry, been increasing recent years, but the toxicological impact of nanoparticles has rarely studied plants. •We exposed Arabidopsis rice leaf protoplasts to SWCNTs examined cell viability, DNA damage, reactive oxygen species generation, related gene expression. We also tested effects on leaves after injecting a SWCNT...
Banana is an important staple crop and widely grown in more than 130 countries around the world. Plant height one of major characteristics crops (Wang et al., 2018); however, commercial banana varieties 2 m, higher are frequently challenged by weak lodging severe damages caused typhoons storms. Dwarf suitable for mechanized plant maintenance fruit harvesting (Dash Rai, 2016); thus, development semi-dwarf dwarf to current farming systems. Gibberellin (GA) most determinants (Sasaki 2002),...
Arabidopsis thaliana plants that lack ceramide kinase, encoded by ACCELERATED CELL DEATH5 (ACD5), display spontaneous programmed cell death late in development and accumulate substrates of ACD5. Here, we compared accumulation kinetics, defense responses, ultrastructural features, sites reactive oxygen species (ROS) production wild-type acd5 during and/or Botrytis cinerea infection. Quantitative sphingolipid profiling indicated paralleled the appearance death, it was accompanied autophagy...
Abstract Background To date, CRISPR/Cas9 RNP editing tools have not been applied to the genetic modification of banana. Here, establishment a PEG-mediated banana protoplast transformation system makes it possible build an efficient DNA-free method for site-directed mutagenesis system. Results Protoplasts constitute versatile platform transient expression in plant science. In this study, we established This was further optimized successfully delivering and CRISPR/Cas12a plasmids...
Banana is a fruit with high nutrient content and economic importance. It regarded as main staple food in developing countries. As typical climacteric fruit, banana will ripen decay one week after exogenous ethylene induction. The short shelf life of largely limits its storage, transportation marketing causes great postharvest loss (Krishnakumar Thirupathi, 2014). closely related to production, which the first factor considered for preservation technology. A reduction endogenous production or...
Summary Fusaric acid ( FSA ) is a phytotoxin produced by several Fusarium species and has been associated with plant disease development, although its role still not well understood. Mutation of key genes in the biosynthetic gene FUB cluster oxysporum f. sp. cubense tropical race 4 Foc TR 4) reduced production, resulted decreased symptoms fungal biomass host banana plants. When pretreated , both leaves pseudostems exhibited increased sensitivity to invasion. Banana embryogenic cell...
Anthocyanins spatiotemporally accumulate in certain tissues of particular species the banana plant, and MYB transcription factors (TFs) serve as their primary regulators. However, precise regulatory mechanism remains to be determined. Here, we report identification characterization MaMYB4 , an R2R3-MYB repressor TF, characterized by presence EAR (ethylene-responsive element binding factor–associated amphiphilic repression) TLLLFR motifs. expression was induced accumulation anthocyanins....
Ceramidases hydrolyze ceramide into sphingosine and fatty acids. In mammals, ceramidases function as key regulators of sphingolipid homeostasis, but little is known about their roles in plants. Here we characterize the Arabidopsis ceramidase AtACER, a homolog human alkaline ceramidases. The acer-1 T-DNA insertion mutant has pleiotropic phenotypes, including reduction leaf size, dwarfing an irregular wax layer, compared with wild-type Quantitative profiling showed that mutants artificial...
Fruit pathogens can contribute to the acidification or alkalinization of host environment. This capability has been used divide fungal into acidifying and/or alkalinizing classes. Here, we show that diverse classes pathogens-Colletotrichum gloeosporioides, Penicillium expansum, Aspergillus nidulans and Fusarium oxysporum-secrete small pH-affecting molecules. These molecules modify environmental pH, which dictates acidic alkaline colonizing strategies, induce expression PACC-dependent genes....
Current banana cultivation is facing a serious threat known as Panama disease or Fusarium wilt, which caused by the oxysporum (Dale et al., 2017). To date, development of wilt-resistant still faces challenges various sexual reproduction barriers, such high sterility, complex genetic background, polypoid, and parthenogenesis (Ghag 2014), making it extremely difficult to develop new disease-resistant bananas via cross-breeding (Czislowski 2018). In recent years, host-induced gene silencing...
MicroRNAs (miRNAs) are a family of non-coding small RNAs that play an important regulatory role in various biological processes. Previous studies have reported miRNAs closely related to the ripening process model plants. However, involved banana fruit remain unknown. Here, we investigated miRNA populations from fruits response ethylene or 1-MCP treatment using deep sequencing approach and bioinformatics analysis combined with quantitative RT-PCR validation. A total 125 known 26 novel were...
Banana (Musa acuminata, AAA group) is a representative climacteric fruit with essential nutrients and pleasant flavors. Control of its ripening determines both the quality shelf life. NAC (NAM, ATAF, CUC2) proteins, as one largest superfamilies transcription factors, play crucial roles in various functions, especially developmental processes. Thus, it important to conduct comprehensive identification characterization factor family at genomic level M. acuminata. In this article, total 181...
Volatile compounds are important factors that affect the flavor quality of banana. The odors banana during harvest change significantly depending upon season; however, nature changes occurring at different times is still unclear. To clarify how harvesting time affects volatile compound profiles and comprehensive characterization, BaXi GuangFen No. 1 cultivars fruit harvests in March September were evaluated using electronic nose technology, also headspace solid phase micro-extraction...
The CRISPR/Cas9-mediated genome editing system has been used extensively to engineer targeted mutations in a wide variety of species. Its application banana, however, hindered because the species’ triploid nature and low efficiency. This delayed development DNA-free approach. In this study, we reported that endogenous U6 promoter banana codon-optimized Cas9 apparently increased mutation frequency generated method validate efficiency based on transient expression protoplasts. activity MaU6c...
Litchi fruit contains abundant polyphenols and is susceptible to browning after harvest. Herein the combined treatments of malic acid (MA) lycopene (LYC) delay development in litchi stored at room temperature (25°C) low (4°C) was investigated. The results showed that pericarp could be alleviated, increase malondialdehyde (MDA) content relative leakage rate retarded by MA LYC during storage. As compared control, anthocyanins, flavonoids, total phenols maintained higher levels; decrease...
Abstract Low temperature is a major environmental factor that limits the growth, yield, and geographical distribution of Cavendish Dajiao bananas ( Musa spp.). exhibit significantly higher cold tolerance than cultivar. However, underlying mechanisms involved in regulation plants have not been determined. In this study, we investigated differences at cellular level between cold-sensitive cold-tolerant banana types through comparative metabolomics transcriptomics analyses calli exposed to...
Ceramidases hydrolyze ceramide into sphingosine and fatty acids and, although ceramidases function as key regulators of sphingolipid homeostasis in mammals, their roles plants remain largely unknown. Here, we characterized the Arabidopsis thaliana ceramidase AtNCER1, a homolog human neutral ceramidase. AtNCER1 localizes predominantly on endoplasmic reticulum. The ncer1 T-DNA insertion mutants had no visible phenotype, but accumulated hydroxyceramides, showed increased sensitivity to...
Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) is well-known as the causal agent of wilt banana and one most destructive phytopathogens for plants. The molecular mechanisms underlying Foc TR4 virulence remain elusive. Here, we demonstrate that a cerato-platanin (CP) protein, FocCP1, functions factor required by penetration full virulence. FocCP1 gene was expressed in every condition studied, showing high transcript level planta at early stage infection. Infiltration recombinant...
Banana is an important tropical fruit with high economic value. One of the main species (cultivar Cavendish) susceptible to low temperature, while another close relative specie (Dajiao), has considerably higher cold tolerance. We have reported that some membrane proteins appear involved in tolerance Dajiao via antioxidation mechanism. To investigate early stress response Dajiao, here we applied comparative proteomics analysis for both cold-sensitive Cavendish and cold-tolerant subjected at...
Ceramide sphingolipids are major components of membranes. C2 and C6 ceramides induce programmed cell death (PCD) in animals plants we previously showed that PCD rice (Oryza sativa) protoplasts. However, the mechanistic link between remains unclear. Here, provide evidenceobserved calcium levels increased rapidly after ceramide treatment.; Moreover, channel inhibitor LaCl3 intracellular chelator acetoxymethyl-1, 2-bis (2-aminophenoxy) ethic acid (BAPTA-AM) inhibited this increase prevented...
Polyphenol oxidases (PPOs), which are widely present in plants, play an important role the growth, development, and stress responses. They can catalyze oxidization of polyphenols result browning damaged or cut fruit, seriously affects fruit quality compromises sale fruit. In banana (Musa acuminata, AAA group), 10 PPO genes were determined based on availability a high-quality genome sequence, but remains unclear.In this study, we analyzed physicochemical properties, gene structure, conserved...