A5029142307- Pharmacogenetics and Drug Metabolism
- Natural product bioactivities and synthesis
- Pharmacological Effects of Natural Compounds
- Drug Transport and Resistance Mechanisms
- Molecular Sensors and Ion Detection
- Phytochemistry and Biological Activities
- Cholinesterase and Neurodegenerative Diseases
- Computational Drug Discovery Methods
- Nanoplatforms for cancer theranostics
- Bioactive Natural Diterpenoids Research
- Traditional and Medicinal Uses of Annonaceae
- Plant biochemistry and biosynthesis
- Adenosine and Purinergic Signaling
- Alkaloids: synthesis and pharmacology
- Eicosanoids and Hypertension Pharmacology
- Advanced biosensing and bioanalysis techniques
- Sulfur Compounds in Biology
- Peptidase Inhibition and Analysis
- Metabolomics and Mass Spectrometry Studies
- Cancer therapeutics and mechanisms
- Click Chemistry and Applications
- RNA modifications and cancer
- Free Radicals and Antioxidants
- Amino Acid Enzymes and Metabolism
- Fungal Biology and Applications
Dalian Medical University
2015-2024
Peking University Shenzhen Hospital
2022-2024
Second Affiliated Hospital of Dalian Medical University
2017-2024
First Affiliated Hospital of Dalian Medical University
2017-2022
Xuzhou Medical College
2019-2021
Genetech (Sri Lanka)
2021
Jiangxi University of Traditional Chinese Medicine
2020-2021
State Council of the People's Republic of China
2021
Dalian University
2015-2020
Dalian University of Technology
2020
Cytochrome P450 2J2 (CYP2J2), a key enzyme responsible for oxidative metabolism of various xenobiotics and endogenous compounds, participates in diverse array physiological pathological processes humans. Its biological role tumorigenesis cancer diagnosis remains poorly understood, owing to the lack molecular tools suitable real-time monitoring CYP2J2 complex systems. Using design principles, we were able modify distance between catalytic unit metabolic recognition moiety, allowing us develop...
Melatonin is present in virtually all organisms from bacteria to mammals, and it exhibits a broad spectrum of biological functions, including synchronization circadian rhythms oncostatic activity. Several functions melatonin are mediated by its membrane receptors, but others receptor-independent. For the latter, required penetrate enters intracellular compartments. However, mechanism which cells remains debatable. In this study, was identified that sulfation metabolites were substrates...
Carboxylesterase 2 (CES2), an endoplasmic reticulum (ER) located phase I enzyme, plays a vital role in the metabolism of various endogenous and exogenous substances, is regarded as important target for design prodrugs. Unfortunately, superior highly selective ER targeting fluorescent probes monitoring CES2 are not currently available. Herein, we report sensitive ratiometric probe ERNB based on localizing group p-toluenesulfonamide. possessed high specificity, sensitivity, exhibited excellent...
β-Glucuronidase (GLU) is an important biomarker for primary cancers and intestinal metabolism of drugs or endogenous substances; however, effective optical probe near-infrared (NIR) monitoring in vivo still lacking. Herein, we design enzyme-activated off-on NIR fluorescent probe, HC-glu, based on a hemicyanine keleton, which conjugated with d-glucuronic acid residue via glycosidic bond, the quantification trapping GLU activity vitro vivo. The newly developed exhibited prominent features...
Rational design of practical probes with excellent specificity and improved optical properties for a particular enzyme is always big challenge. Herein, highly specific fluorescent probe carboxylesterase 1 (CES1) was rationally designed using meso-carboxyl-BODIPY as the basic fluorophore based on substrate preference catalytic CES1. Following molecular docking-based virtual screening combined reaction phenotyping-based experimental screening, we found that MMB (probe 7) exhibited optimal...
UDP-glucuronosyltransferase 1A1 (UGT1A1) is a vital metabolic enzyme responsible for the clearance of endogenous substances and drugs. Hitherto, development fluorescent probes UGTs was severely restricted due to poor isoform selectivity on-off or blue-shifted fluorescence response. Herein, we established novel "molecular-splicing" strategy construct highly selective near-infrared (NIR) probe, HHC, UGT1A1, which exhibited NIR signal at 720 nm after UGT1A1 metabolism. HHC then successfully...
Abstract NEMO/IKKβ complex is a central regulator of NF-κB signaling pathway, its dissociation has been considered to be an attractive therapeutic target. Herein, using combined strategy molecular pharmacological phenotyping, proteomics and bioinformatics analysis, Shikonin (SHK) identified as potential inhibitor the IKKβ/NEMO complex. It destabilizes with IC 50 174 nM, thereby significantly impairing proliferation colorectal cancer cells by suppressing pathway in vitro vivo. In addition, we...
Inhibition of ferroptosis in intestinal epithelial cells ameliorates clinical symptoms and improves endoscopic presentations inflammatory bowel disease (IBD). Licorice is used worldwide food medicine fields. Liquiritin, a flavonoid component licorice, an effective substance as anti-inflammatory, antioxidant that has been shown to improve chemically induced colitis. Herein we evaluated the therapeutic effects liquiritin on colitis determined whether could affect by modulating cells. A model...
Shaoyao Decoction (SYD) is a canonical herbal medicine prescription formulated by Liu Wan-Su in AD 1186. SYD has been widely used to treat inflammatory bowel disease clearing heat and damp, removing stasis toxin the intestine; however, precise mechanisms therapeutic material basis remain largely unclear. In present study, we measured effects of on colitis symptom, epithelial barrier function, ferroptosis, colonic protein mRNA expression glutathione peroxidase 4 (GPX4) model, determined...
Objective Dysbiosis of the intestinal fungal community has been observed in inflammatory bowel disease (IBD); however, its potential role IBD development and prevention remains unclear. Here, we explored biological effects molecular mechanisms fungi isolated from human faeces on colitis mice. Design Intestinal strains with differential abundance were cultivated their various mouse models experimental evaluated. In addition, bioactive metabolites secreted by target fungus accurately...
An investigation on the bioactive chemical constituents of roots Euphorbia fischeriana has been conducted, with 21 diterpenoids obtained using various chromatographic techniques. On basis spectroscopic data analysis, new compounds were elucidated as four ent-abietane-type (1–4) and tigliane-type (13–16). Also eight known ent-abietane (5–12) five tigliane (17–21) diterpenoids. The potential antituberculosis effects these evaluated a Mycobacterium smegmatis model. most potent compound...
Abstract Background Glioblastoma multiforme (GBM) is one of the most refractory and palindromic central nervous system (CNS) neoplasms, current treatments have poor effects in GBM patients. Hence, identification novel therapeutic targets development effective treatment strategies are essential. Alantolactone (ATL) has a wide range pharmacological activities, its anti-tumor effect receiving increasing attention. However, molecular mechanism underlying anti-GBM activity ATL remains poorly...
Bacterial γ-glutamyltranspeptidases (γ-GT) is a well-known metabolic enzyme, which could cleave the γ-glutamyl amide bond of analogues. As key enzyme bacteria and virulence factor for host, bacterial γ-GT was determined to be novel pharmaceutical target new antibiotics development. However, there no efficient method sensing activity in recognition γ-glutamyltransferase rich-bacteria. In present work, dicyanoisophorone derivative (ADMG) has been designed developed sensitive selective...
Visualization of endogenous disease-associated enzymes is great clinical significance, as it could allow earlier diagnosis and timely intervention. Herein, we first synthesized characterized an enzyme-activatable near-infrared fluorescent probe, GP-DM, for determining the activity dipeptidyl peptidase IV (DPP IV), which associated with various pathological processes, especially in diabetes malignant tumors. GP-DM emitted significant turn-on NIR signals simultaneously response to DPP IV,...
Carboxylesterase 2 (CES 2) is a key enzyme in the activation of prodrug irinotecan (CPT-11) treatment against colorectal cancer and also has some relationship with side effect CPT-11 clinical applications.
Vanin-1 is an amidohydrolase that catalyses the conversion of pantetheine into amino-thiol cysteamine and pantothenic acid (coenzyme A precursor), which plays a vital role in multiple physiological pathological processes. In this study, enzyme-activated near-infrared (NIR) fluorescent probe (DDAV) has been constructed for sensitively detecting activity complicated biosamples on basis its catalytic characteristics. DDAV exhibited high selectivity sensitivity toward was successfully applied to...
Abstract Background The gut virome has been implicated in inflammatory bowel disease (IBD), yet a full understanding of the IBD patients, especially across diverse geographic populations, is lacking. Results In this study, we conducted comprehensive virome-wide association study Chinese cohort 71 patients (15 with Crohn’s and 56 ulcerative colitis) 77 healthy controls via viral-like particle (VLP) bulk sequencing their feces. By utilizing an integrated virus catalog tailored to virome,...
Melatonin is an endogenous indoleamine with a wide range of biological functions in the various organisms from bacteria to mammals. Evidence indicates that melatonin facilitates apoptosis cancer cells and enhances antitumor activity chemotherapy animals clinical studies. However, metabolism key metabolic targets still remain unknown. In this study, U118 SH-SY5Y tumor cell lines were used investigate pathways cells. Interestingly, inhibitory effect on proliferation more potent than contrast,...
Background/Aims: Uncaria rhynchophylla, known as “Gou-teng”, is a traditional Chinese medicine (TCM) used to extinguish wind, clear heat, arrest convulsions, and pacify the liver. Although U. rhynchophylla has long history of being often treat central nervous system (CNS) diseases, its efficacy potential mechanism are still uncertain. This study investigated neuroprotective effect underlying extract (URE) in MPP+-induced SH-SY5Y cells MPTP-induced mice. Methods: mice were established...