A5047235136- Immune Response and Inflammation
- Cholesterol and Lipid Metabolism
- Immune cells in cancer
- interferon and immune responses
- Immune Cell Function and Interaction
- Asthma and respiratory diseases
- Phagocytosis and Immune Regulation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neonatal Respiratory Health Research
- Inflammasome and immune disorders
- Respiratory Support and Mechanisms
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Peroxisome Proliferator-Activated Receptors
- Sphingolipid Metabolism and Signaling
- Atherosclerosis and Cardiovascular Diseases
- Cytokine Signaling Pathways and Interactions
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Adipokines, Inflammation, and Metabolic Diseases
- Lipoproteins and Cardiovascular Health
- Immunotherapy and Immune Responses
- Sepsis Diagnosis and Treatment
- Drug Transport and Resistance Mechanisms
- Fatty Acid Research and Health
- Caveolin-1 and cellular processes
- Cancer, Lipids, and Metabolism
National Institutes of Health
2015-2025
National Institute of Environmental Health Sciences
2016-2025
Triangle
2010-2025
Research Triangle Park Foundation
2008-2025
Immunité et Cancer
2017-2023
The Ohio State University Wexner Medical Center
2021
The Centers
2020
Community Link
2020
Orszagos Kornyezetegeszsegugyi Intezet
2019
University of Minnesota System
2017
We previously showed that macrophages from macrophage-specific ATP-binding cassette transporter A1 (ABCA1) knockout (Abca1(-M/-M)) mice had an enhanced proinflammatory response to the Toll-like receptor (TLR) 4 agonist, lipopolysaccharide (LPS), compared with wild-type (WT) mice. In present study, we demonstrate a direct association between free cholesterol (FC), lipid raft content, and hyper-responsiveness of LPS in WT Abca1(-M/-M) were also hyper-responsive specific agonists TLR2, TLR7,...
Macrophage activation and infiltration into resident tissues is known to mediate local inflammation a hallmark feature of metabolic syndrome. Members the sirtuin family proteins regulate numerous physiological processes, including those involved in nutrient regulation promotion longevity. However, important role that SIRT1, leading member, plays immune response remains unclear. In this study, we demonstrate SIRT1 modulates acetylation status RelA/p65 subunit NF-κB thus pivotal regulating...
Advancements in methods, technology, and our understanding of the pathobiology lung injury have created need to update definition experimental acute (ALI). We queried 50 participants with expertise ALI respiratory distress syndrome using a Delphi method composed series electronic surveys virtual workshop. propose that presents as “multidimensional entity” characterized by four “domains” reflect key pathophysiologic features underlying biology human syndrome. These domains are 1) histological...
Cystic fibrosis (CF) lung disease features persistent neutrophil accumulation to the airways from time of infancy. CF children are frequently exposed Pseudomonas aeruginosa, and by adulthood, 80% patients chronically infected. The formation biofilms is a particularly important phenotypic characteristic P. aeruginosa that allows for bacterial survival despite aggressive antibiotic therapy an exuberant immune response. Here, we show presence neutrophils enhances initial biofilm development...
Lymphoid interstitial pneumonia (LIP) is rare and its clinical course incompletely described. The aim of this study was to examine the features, associations prognosis surgical lung biopsy-proven LIP. group consisted 15 subjects encountered over a 14-yr period. majority were females (n = 11) mean age 47 yrs (range 17–78 yrs). Underlying systemic immune disorders frequent, including Sjögren's syndrome 8), rheumatoid arthritis, lupus erythematosus, polymyositis, common variable...
Abstract Statins are potent, cholesterol-lowering agents with newly appreciated, broad anti-inflammatory properties, largely based upon their ability to block the prenylation of Rho GTPases, including RhoA. Because phagocytosis apoptotic cells (efferocytosis) is a pivotal regulator inflammation, which inhibited by RhoA, we sought determine whether statins enhanced efferocytosis. The effect lovastatin on efferocytosis was investigated in primary human macrophages, murine lung, and alveolar...
Abstract Macrophages are sentinel immune cells that survey the tissue microenvironment, releasing cytokines in response to both exogenous insults and endogenous events such as tumorigenesis. mediate tumor surveillance therapy-induced regression; however, tumor-associated macrophages (TAM) their products may also promote progression. Whereas NF-κB is prominent macrophage-initiated inflammatory responses, little known about role of p53 macrophage responses environmental challenge, including...
In recent years the functions that p53 tumor suppressor plays in human biology have been greatly extended beyond "guardian of genome." Our studies promoter response element sequences targeted by master regulatory transcription factor suggest a general role for this DNA damage and stress-responsive regulator control Toll-like receptor (TLR) gene expression. The TLR family mediates innate immunity to wide variety pathogenic threats through recognition conserved pathogen-associated molecular...
Lipid-laden macrophages, or "foam cells," are observed in the lungs of patients with fibrotic lung disease, but their contribution to disease pathogenesis remains unexplored. Here, we demonstrate that fibrosis induced by bleomycin, silica dust, thoracic radiation promotes early and sustained accumulation foam cells lung. In bleomycin model, show arise from neighboring alveolar epithelial type II cells, which respond injury dumping lipids into distal airspaces lungs. We oxidized phospholipids...
Abstract Dendritic cells (DC) in the lung that induce Th17 differentiation remain incompletely understood, part because conventional CD11b + DCs (cDC2) are heterogeneous. Here, we report a population of cDCs rapidly accumulates lungs mice following house dust extract inhalation. These Ly-6C , developmentally and phenotypically similar to cDC2, strongly promote ex vivo. Single cell RNA-sequencing (scRNA-Seq) cDC2 indicates 5 distinct clusters. Pseudotime analysis scRNA-Seq data adoptive...
Despite the prominent use of complex survey data and growing popularity machine learning methods in epidemiologic research, few software implementations offer options for handling samples. A major challenge impeding broader incorporation into research is incomplete guidance analyzing data, including importance sampling weights valid prediction target populations. Using from 15, 820 participants 1988–1994 National Health Nutrition Examination Survey cohort, we determined whether ignoring...
Bacterial lipopolysaccharide (LPS) evokes several functional responses in the neutrophil that contribute to innate immunity. Although certain responses, such as adhesion and synthesis of tumor necrosis factor-α, are inhibited by pretreatment with an inhibitor p38 mitogen-activated protein kinase, others, actin assembly, unaffected. The aim present study was investigate changes gene transcription expression following exposure establish their dependence on signaling. Microarray analysis...
Rationale: A growing literature indicates that hydroxy-methylglutaryl coenzyme reductase inhibitors (statins) modulate proinflammatory cellular signaling and functions. No studies to date, however, have addressed whether statins pulmonary inflammation triggered by aerogenic stimuli or they affect host defense. Objectives: To test lovastatin modulates LPS-induced antibacterial Methods: address these questions, confirm any effect of as dependent on inhibition reductase, we treated C57Bl/6 mice...