A5008781594- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Cellular transport and secretion
- Pancreatic function and diabetes
- Advanced Electron Microscopy Techniques and Applications
- Photoreceptor and optogenetics research
- Optical Coherence Tomography Applications
- Near-Field Optical Microscopy
- Digital Holography and Microscopy
- Metabolism, Diabetes, and Cancer
- Spectroscopy Techniques in Biomedical and Chemical Research
- Image Processing Techniques and Applications
- AI in cancer detection
- Immunotherapy and Immune Responses
- Photoacoustic and Ultrasonic Imaging
- Advanced X-ray Imaging Techniques
- Lipid Membrane Structure and Behavior
- Cellular Mechanics and Interactions
- Optical measurement and interference techniques
- Human Pose and Action Recognition
- Microtubule and mitosis dynamics
- bioluminescence and chemiluminescence research
- Photonic and Optical Devices
- Molecular Communication and Nanonetworks
- Nanoplatforms for cancer theranostics
Children's Hospital of Zhejiang University
2022-2025
Zhejiang University
2016-2025
State Key Laboratory of Modern Optical Instruments
2021-2025
Zhejiang University of Technology
2021-2024
ZheJiang Institute For Food and Drug Control
2023
Sir Run Run Shaw Hospital
2018-2023
Yale University
1992-2023
Alibaba Group (China)
2021-2022
Ministry of Education of the People's Republic of China
2014-2020
ShanghaiTech University
2020
The first community competition designed to objectively compare the performance of particle tracking algorithms provides valuable practical information for both users and developers. Particle is key importance quantitative analysis intracellular dynamic processes from time-lapse microscopy image data. Because manually detecting following large numbers individual particles not feasible, automated computational methods have been developed these tasks by many groups. Aiming perform an objective...
Abstract Structured illumination microscopy (SIM) surpasses the optical diffraction limit and offers a two-fold enhancement in resolution over limited microscopy. However, it requires both intense multiple acquisitions to produce single high-resolution image. Using deep learning augment SIM, we obtain five-fold reduction number of raw images required for super-resolution generate under extreme low light conditions (at least 100× fewer photons). We validate performance neural networks on...
Solid lipid nanoparticles (SLNs) have been extensively investigated and demonstrated to be a potential nanocarriers for improving oral bioavailability of many drugs. However, the molecular mechanisms related this discovery are not yet understood. Here, transport SLNs crossing simulative intestinal epithelial cell monolayers (Caco-2 monolayers) were studied. The cytotoxicology results in Caco-2 cells that had low cytotoxicity, no effect on integrity membrane, did induce oxidative stress,...
Insulin stimulates translocation of GLUT4 storage vesicles (GSVs) to the surface adipocytes, but precisely where insulin acts is controversial. Here we quantify size, dynamics, and frequency single vesicle exocytosis in 3T3-L1 adipocytes. We use a new GSV reporter, VAMP2-pHluorin, bypass signaling by disrupting GLUT4-retention protein TUG. Remarkably, unstimulated TUG-depleted cells, exocytic rate similar that insulin-stimulated control cells. In triggers transient, twofold burst exocytosis....
Background: In recent decades, long non-coding RNAs (lncRNAs) have been reported as crucial functional regulators involved in ovarian cancer.In the present study, we explored how lncRNA RHPN1-AS1 influences progression of epithelial cancer (EOC) through tumor cell-dependent mechanisms.Results: The expression EOC tissues was higher than that para-cancerous control tissues.High closely associated with poor prognosis patients.N6methyladenosine (m6A) improved stability methylation transcript by...
The formation of mature secretory granules is essential for proper storage and regulated release hormones neuropeptides. In pancreatic β cells, cholesterol accumulation causes defects in insulin secretion may participate the pathogenesis type 2 diabetes. Using a novel analog, we show first time that are major sites intracellular live cells. This distinct from other, non-secretory cell types, which concentrated recycling endosomes trans-Golgi network. Excess was delivered specifically to...
Here we demonstrate an active method which pioneers in utilizing a combination of spatial frequency shift and Stokes to enable wide-field far-field subdiffraction imaging. A fluorescent nanowire ring acts as localized source is combined with film waveguide produce omnidirectional illuminating evanescent waves. Benefitting from the high wave vector illumination, frequencies object can be shifted passband conventional imaging system, contributing subwavelength information image. structure...
The balance of mitochondrial fission and fusion plays an important role in maintaining the stability cellular homeostasis. Abnormal fragmentation have been shown to be associated with oxidative stress, which causes a variety human diseases from neurodegeneration disease cancer. Therefore, induction aggregation may provide alternative approach alleviate these conditions. Here, optogenetic-based system (Opto-MitoA) developed, is based on CRY2clust/CIBN light-sensitive module. Upon blue light...
Imaging and tracking of near-surface three-dimensional volumetric nanoscale dynamic processes live cells remains a challenging problem. In this paper, we propose multi-color live-cell near-surface-volume super-resolution microscopy method that combines total internal reflection fluorescence structured illumination with multi-angle evanescent light illumination. We demonstrate our approach interference is perfectly adapted to studying subcellular dynamics mitochondria microtubule...
ABSTRACT Glucose transporter 4 (GLUT4; also known as SLC2A4) resides on intracellular vesicles in muscle and adipose cells, translocates to the plasma membrane response insulin. The phosphoinositide 3-kinase (PI3K)–Akt signaling pathway plays a major role GLUT4 translocation; however, challenge has been unravel potentially distinct contributions of PI3K Akt (of which there are three isoforms, Akt1–Akt3) overall insulin action. Here, we describe new optogenetic tools based CRY2 N-terminus...
We investigate experimentally and computationally the OH CH radical fields in a twodimensional, axisymmetric, laminar, methane-air diffusion flame which cylindrical fuel stream is surrounded by coflowing oxidizer jet. Experimentally, laser induced fluorescence used to generate profiles of minor species. Computationally, we apply detailed chemistry, complex transprot combustion model predict temperature, veocity species concentrations as function two coordinate directions. Unlike some models...
The fast imaging speed and low-intensity requirement of structured illumination microscopy (SIM) have made it one the most widely used tools in live cell imaging. In order to obtain a high fidelity reconstructed image, precise estimation phase pattern is required, especially those based techniques that rely on high-order harmonics improve resolution. This can be achieved two fundamental ways. first build high-end control system capable shifting sinusoidal with precision, while second apply...
We report a novel superresolution microscopy approach for imaging fluorescence samples. The reported approach, termed virtual k-space modulation optical (VIKMOM), is able to improve the lateral resolution by factor of 2, reduce background level, sectioning effect and correct unknown aberrations. In acquisition process VIKMOM, we used scanning confocal microscope setup with 2D detector array capture sample information at each scanned x-y position. recovery first modulated captured data coding...
Measuring three-dimensional nanoscale cellular structures is challenging, especially when the structure dynamic. Owing to informative total internal reflection fluorescence (TIRF) imaging under varied illumination angles, multi-angle (MA) TIRF has been examined offer a axial and subsecond temporal resolution. However, conventional MA-TIRF still performs badly in lateral resolution fails characterize depth image densely distributed regions. Here, we emphasize super-resolution MA-TIRF,...
Type 2 diabetes is caused by defects in both insulin sensitivity and secretion. Glucose triggers secretion causing exocytosis of granules from pancreatic β-cells. High circulating cholesterol levels a diminished capacity serum to remove β-cells are observed diabetic individuals. Both these effects can lead accumulation contribute β-cell dysfunction. However, the molecular mechanisms which impairs function remain largely unknown. Here, we used total internal reflection fluorescence microscopy...
Benefiting from the advanced human visual system, humans naturally classify activities and predict motions in a short time. However, most existing computer vision studies consider those two tasks separately, resulting an insufficient understanding of actions. Moreover, effects view variations remain challenging for skeleton-based methods, graph operators cannot fully explore multiscale relationship. In this article, versatile graph-based model (Vers-GNN) is proposed to deal with...