A5001620569- Neuroinflammation and Neurodegeneration Mechanisms
- Tryptophan and brain disorders
- Parkinson's Disease Mechanisms and Treatments
- Immune Cell Function and Interaction
- Nuclear Receptors and Signaling
- Nerve injury and regeneration
- Receptor Mechanisms and Signaling
- Stress Responses and Cortisol
- Immune Response and Inflammation
- RNA regulation and disease
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neurological Disease Mechanisms and Treatments
- biodegradable polymer synthesis and properties
- Ferrocene Chemistry and Applications
- Blood disorders and treatments
- Protein Kinase Regulation and GTPase Signaling
- Neurogenesis and neuroplasticity mechanisms
- Neuroendocrine regulation and behavior
- Mechanical Circulatory Support Devices
- Patient Satisfaction in Healthcare
- Neurological Disorders and Treatments
- Restless Legs Syndrome Research
- Conducting polymers and applications
- Neurotransmitter Receptor Influence on Behavior
- HIV Research and Treatment
University of Florida
2018-2024
Florida College
2018-2024
Institute for Neurodegenerative Disorders
2021
Garvan Institute of Medical Research
1993
Shell (Netherlands)
1967
Parkinson's disease (PD) is marked by a loss of dopamine neurons, decreased transporter (DAT) and tyrosine hydroxylase (TH) expression. However, this validation approach cannot be used for diagnostic, drug effectiveness or investigational purposes in human patients because midbrain tissue accessible postmortem. PD pathology affects both the central nervous peripheral immune systems. Therefore, we immunophenotyped blood samples presence myeloid derived suppressor cells (MDSCs) discovered that DAT
Monocyte-derived macrophages (MDMs) are key players in tissue homeostasis and diseases regulated by a variety of signaling molecules. Recent literature has highlighted the ability for biogenic amines to regulate macrophage functions, but mechanisms governing amine around immune cells remain nebulous. In CNS, transporters regarded as master regulators neurotransmitter signaling. While we others have shown that express these transporters, relatively little is known their function cells. To...
The dopamine transporter (DAT) regulates the dimension and duration of transmission. DAT expression, its trafficking, protein–protein interactions, activity are conventionally studied in CNS within context neurological diseases such as Parkinson’s Diseases neuropsychiatric drug addiction, attention deficit hyperactivity autism. However, is also expressed at plasma membrane peripheral immune cells monocytes, macrophages, T-cells, B-cells. via an autocrine/paracrine signaling loop macrophage...
Abstract Pathophysiological changes in dopamine neurons precede their demise and contribute to the early phases of Parkinson’s disease (PD). Intracellular pathological inclusions protein α-synuclein within dopaminergic are a cardinal feature PD, but mechanisms by which contributes neuron vulnerability remain unknown. The inaccessibility diseased tissue has been limitation studying progression pathophysiology prior degeneration neurons. To address these issues, we differentiated induced...
Most, if not all, peripheral immune cells in humans and animals express tyrosine hydroxylase (TH), the rate limiting enzyme catecholamine synthesis. Since TH is typically studied context of brain signaling, little known about changes production function cells. This knowledge gap due, part, to lack an adequately sensitive assay measure expressing lower levels compared other Here, we report development a highly reproducible Bio-ELISA quantify picogram multiple model systems. We have applied...
Background The University of Florida (UF) Equal Access Clinic Network (EACN) is the largest student-run free healthcare clinic network in Florida. UF EACN serves those who are underinsured or uninsured Alachua County and its surrounding area. Nationally, average total time per medical visit has been established to be 84 min. Problem Before this project, patient cycle at was 125.3 min, there no quality improvement (QI) team implement changes address inefficiencies. Methods This a prospective...
Deposition of misfolded α-synuclein (αsyn) in the enteric nervous system (ENS) is found multiple neurodegenerative diseases. It hypothesized that ENS synucleinopathy contributes to both pathogenesis and non-motor morbidity Parkinson's Disease (PD), but cellular molecular mechanisms shape histopathology dysfunction are poorly understood. Here, we demonstrate ENS-resident macrophages, which play a critical role maintaining homeostasis, initially respond neuronal αsyn pathology by upregulating...
Prenatal care is a cornerstone of maternal and child health. This paper describes the creation free prenatal clinic for uninsured families following recognition significant disparity in birth outcomes Alachua County, Florida including: development essential community resource collaborations, clinical operations dual aim patient care-medical education site, preliminary after one year operation, identified challenges, next steps our growing program. A retrospective chart review all sixteen...
Abstract Monocyte-derived macrophages are key players in tissue homeostasis and disease regulated by a variety of signaling molecules. Recent literature has highlighted the ability for biogenic amines to regulate macrophage functions, but mechanisms governing amine on around immune cells remains nebulous. In central nervous system, transporters regarded as master regulators neurotransmitter signaling. While we others have shown express these transporters, relatively little is known their...
<b>Abstract ID 52323</b> <b>Poster Board 370</b> Dopamine transporter (DAT) is a master regulator of dopamine transmission in the brain. Mutation DAT structure implicated familial Parkinson's disease. In addition, one main targets psychostimulants, therefore, conventionally studied central nervous system (CNS) and context neurological neuropsychiatric diseases. But, addition to brain, also expressed at plasma membrane myeloid lymphoid cells periphery. We found activity reduces macrophage...
<b>Abstract ID 54347</b> <b>Poster Board 536</b> The dopamine transporter (DAT) regulates the dimension and duration of transmission. DAT expression, its trafficking, protein–protein interactions, activity are conventionally studied in CNS within context neurological diseases such as Parkinson9s Diseases neuropsychiatric drug addiction, attention deficit hyperactivity autism. However, is also expressed at plasma membrane peripheral immune cells monocytes, macrophages, T-cells, B-cells. via...
Parkinson's Disease (PD), the second‐most common neurodegenerative disorder, has three key hallmarks: dysregulated dopamine (DA) signaling in part due to degeneration of Substantia Nigra neurons, intracellular aggregates a‐synulcein, and neuroinflammation. However, origin interplay these features remains unclear. DA substitution therapy, standard pharmacological treatment, only alleviates symptoms with limited efficacy. Interestingly, plasma levels are altered PD, can regulate immune cell...
Introduction Parkinson's Disease (PD) is characterized by loss of substantia nigra (SN) dopaminergic neurons, resulting in reduced CNS dopamine transmission. PD widely thought to start the periphery; however, whether and how affects peripheral transmission remains unknown. Peripheral immune cells express key proteins, including transporter (DAT) tyrosine hydroxylase (TH). Therefore, it possible that system affected pathology. Objective We hypothesized patients exhibit dysregulated...
May 9, 2019April 2019Free AccessAltered Dopamine Transmission in Peripheral Mononuclear Cells Parkinson’s Disease (P5.8-028)Leila Saadatpour, Adithya Gopinath, Phillip Mackie, Subhashie Wijemanne, and Habibeh KhoshboueiAuthors Info & AffiliationsApril 2019 issue92 (15_supplement)https://doi.org/10.1212/WNL.92.15_supplement.P5.8-028 Letters to the Editor
ABSTRACT IMPACT: We present preliminary data and an outlined approach to assess peripheral immune changes associated with PTSD in a clinical setting pre-clinical rat model of PTSD. OBJECTIVES/GOALS: report our methodology findings indicating relationship between CNS dopamine signaling cell populations propose extend this patient population elucidate immune-brain connections disorder. METHODS/STUDY POPULATION: Using IRB-approved protocol collaboration board-certified psychiatrist, we will...
Abstract Most, if not all, peripheral immune cells in humans and animals express tyrosine hydroxylase (TH), the rate limiting enzyme catecholamine synthesis. Since TH is typically studied context of brain signaling, little known about changes production function cells. This knowledge gap due, part, to lack an adequately sensitive assay measure expressing lower levels compared other Here, we report development a highly reproducible Bio-ELISA quantify picogram multiple model systems. We have...
Abstract Chest radiography compares left ventricular decompression in the same patient supported with extracorporeal membrane oxygenation atrial septal fenestration and subsequently assist device apical cannulation.
Neuromodulators, such as norepinephrine and dopamine, regulate immune responses. In addition to receptor competency, select cells express machinery engage in active neurotransmitter transmission. Specifically, macrophages the (NET) dopamine transporters (DAT). However, how these - classically studied central neurons affect immunity remains poorly understood. To this end, we investigated expression function of NET DAT human macrophages. We found that monocyte-derived functional vitro. A...