Radiation exposure negatively affects the regenerative ability and makes reconstruction of bone defects after tumor section difficult. miR-34a is involved in radiation biology metabolism. The aim this study was to investigate whether could contribute regeneration irradiated defects. expression analyzed during osteoblastic differentiation BMSCs formation mimics inhibitor were used upregulate or suppress with 2 4 Gy X-ray radiation. In vitro osteogenesis subcutaneous assess effects on...

Polyether-ether-ketone (peek) is one of the most common materials used for load-bearing orthopedic devices owing to its radiolucency and favorable mechanical properties. However, current smooth-surfaced peek implants can lead fibrous capsule formation. To overcome this issue, here, specimens with well-defined internal cross-linked structures (macropore diameters 1.0-2.0 mm) were fabricated using a three-dimensional (3D) printer, an acid-etched microporous surface was achieved...

Background: This study aimed to investigate the biomechanical behaviors of polyether ether ketone (PEEK) and traditional materials (titanium fiber) when used restore tooth defects in form prefabricated post or customized via computational modelling. Methods: First, prototype natural tooth, prototypes were established, respectively, whilst residual root was restored with dentin ferrule using reverse engineering methods. Then, stress strain CFR-PEEK (PEEK reinforced by 30% carbon pure PEEK...

Background Non-invasive imaging technologies for assessing axillary lymph node (ALN) metastasis of breast cancer are needed in clinical practice. Purpose To explore the value intravoxel incoherent motion (IVIM) and diffusional kurtosis (DKI) predicting ALN cancer. Material Methods A total 194 patients with pathologically confirmed who underwent IVIM DKI examination were reviewed retrospectively. The derived parameters D, D*, f DKI-derived MD MK measured. independent samples t-test was used...

ABSTRACT Background Functional anorectal pain (FAP) is classified as one of the disorders gut‐brain interaction (DGBI). It involves impairments afferents and disrupted communication. However, neuroimaging studies focused on FAP are lacking. Methods A total 25 patients 18 healthy controls (HC) underwent structural magnetic resonance imaging (MRI), diffusion tensor (DTI), resting‐state functional MRI (rs‐fMRI) scans, collection demographic data, mental health assessment scales questionnaires....

We generated an induced pluripotent stem cell (iPSC) line from peripheral blood mononuclear cells obtained a 41-year-old male patient diagnosed with schizophrenia using non-integrating reprogramming techniques. The iPSC lines exhibited normal karyotypes, expressed specific markers of pluripotency, and demonstrated the capacity to differentiate into all three germ layers in vitro. This will serve as valuable vitro model for investigating underlying pathophysiological mechanisms this disease...

Background/Aims: Hepatocellular carcinoma (HCC) represents the most common type of liver cancer. DAX1 (dosage-sensitive sex reversal adrenal hypoplasia congenital critical region on X chromosome, gene 1), an atypical member nuclear receptor family due to lack classical DNA-binding domains, has been known for its fundamental roles in development, especially determination and steroidogenesis. Previous studies also showed that DAX-1 played a role endocrine steroid-dependent neoplasms such as...

The primary objective of the present study was to develop extended-release matrix formulations apremilast for oral delivery and their in vitro vivo correlation. Five containing hydroxypropylmethylcellulose (HPMC) as retarding excipient with different release rate were prepared. Dissolution tests all formulated tablets performed water, pH 4.0 6.8 buffer solutions. kinetics studied supported by Korsmeyer-Peppas's equation it presented highest values correlation coefficients (r2 up 0.966)....

Heart failure (HF) is a progressive disease with relatively poor prognosis and lacks effective therapy, the discovery of dysregulated microRNAs (miRNAs) their role in cardiac fibroblasts have provided new avenue for elucidating mechanism involved HF.Two datasets GSE53080 GSE57338 were used to screen miRNAs profiling analysis differentially expressed genes (DEGs) HF. QRT-PCR was detect miR-216a between HF healthy controls (HC). Cell counting kit-8 (CCK-8) assay clonogenic analyze effect...

It has been well established that hypoxia significantly increases both cellular and tumor resistance to ionizing radiation. Hypoxia associated radiation known for some time but there limited success in sensitizing cells under hypoxic conditions. These studies show that, when irradiated with low linear energy transfer (LET) gamma-rays, poly (ADP-ribose), polymerase (PARP), Fanconi Anemia (FANC), mutant Chinese Hamster Ovary (CHO) respond similarly the non-homologous end joining (NHEJ)...

Novel glucosyl flavonoids are developed by the addition of glucose to naturally occurring flavonoids. Flavonoids known antioxidants that possess radioprotective properties. In order investigate properties novel flavonoids, in vitro DNA double-strand breaks (DSBs) analysis was carried out. present study, Quercetin, Naringenin, and Hesperetin groups included natural 13 were investigated. mixed with Lambda DNA, subsequently exposed gamma‑rays. Furthermore, DSB yields visualized gel...

X-ray-triggered scintillators (Sc) and photosensitizers (Ps) have been developed for X-ray-induced photodynamic therapy (X-PDT) to selectively destruct deep tissue tumors with a low X-ray dose. This study designed terbium (Tb)-rose bengal (RB) coordination nanocrystals (T-RBNs) by solvothermal treatment, aiming reduce photon energy dissipation between Tb3+ RB thus increase the reactive oxygen species (ROS) production efficiency. T-RBNs synthesized at molar ratio of [RB]/[Tb] = 3 exhibited...

In order to investigate the effect of a copper (II) complexes coumarin derivative and phenanthroline (hereinafter referred as coumarin-copper drug) on lung adenocarcinoma cells in vivo vitro, along with mechanism action, LA795 were treated different concentrations drug. An MTT assay was performed determine cell proliferation ratio, apoptosis detected by Annexin V/propidium iodide staining flow cytometric analysis western blot employed evaluate expression levels apoptosis-associated proteins....

The use of scintillating nanoparticles (ScNPs) in X-ray-induced photodynamic therapy (X-PDT) is a technique for deep tissue-localized tumor with few side effects. ScNPs transfer energy to photosensitizers, which generate massive amounts reactive oxygen species (ROS) and kill cancer cells. Here we fabricated rose bengal (RB)-installed, Tb3+-rich NaYF4 nanocrystals (NaYF4:Tb@RB), optically inert Y3+ enables highly efficient via high Tb3+ doping. NaYF4:Tb was prepared solvothermal synthesis...

Vascular endothelial growth factor (VEGF) is a potent stimulator of angiogenesis and prognostic for many tumors, including those endocrine-responsive tissues such as the breast uterus. In this study, we found that overexpression PEA3 could increase VEGF mRNA levels promoter activity in human T47D SKBR3 cancer cells. Chromatin immunoprecipitation assay demonstrated bind to cells transfected with expression vector. small interfering RNA attenuated binding These results indicated activate transcription.

A serial of mixed-ligand Cu(II) complexes the type [Cu(phens)(H2 PDILeu)]H2 O (1-4) containing phens as 2,2'-bipyridyl (bpy, 1), 1,10-phenanthroline (phen, 2), dipyrido[3,2-d:2',3'-f]quinoxaline (dpq, 3), and dipyrido[3,2-a:2',3'-c]phenazine (dppz, 4) have been isolated characterized. The interaction with calf-thymus DNA has explored by physical methods to propose modes binding complexes, which indicate that 4 interacts more strongly than all other through intercalation interaction....

Pulse radiolysis was employed to investigate fundamental radiation chemical reactions, which are essential in the protection of DNA. Two positively charged peptides (PCPs), histidine-tyrosine-histidine (His-Tyr-His) and lysine-tyrosine-lysine (Lys-Tyr-Lys), as well amino acids that constitute them, were involved. The reaction rate constants for tyrosine (Tyr), histidine (His), lysine (Lys), His-Tyr-His, Lys-Tyr-Lys with OH radicals (•OH) (1.6 ± 0.3) × 1010, (9.0 0.9) 109, (1.4 (1.8 0.1) (1.0...

Tumor hypoxia represents a major challenge to achieving successful therapy outcomes with photodynamic (PDT). We hypothesized that systemic loading of dual porphyrins, protoporphyrin IX (PPIX) as photosensitizer (PS) and hemin (Fe3+‐PPIX) an oxygen generator, onto Eu‐doped NaYF4 scintillator (Sc), collectively terms Eu‐PPIX@Hemin, could enhance the activity X‐ray mediated PDT. Catalase‐like property in presence H2O2 facilitated production molecules (3O2) hypoxic cancer cells. The produced 3O2...

Nanoparticle-based drug delivery systems (DDS) have been developed as effective diagnostic and low-dose imaging agents. Nano-imaging agents with particles greater than 100 nm are difficult to accumulate in pancreatic cancer cells, making high-intensity of challenging. Peptides composed histidine glycine were designed synthesized. Additionally, aqueous peptide solutions irradiated γ-rays produce nanogels an average size 25-53 nm. The mechanisms underlying radiation-mediated crosslinking...

H2BK12ac is an important histone acetylation pattern of H2B, which has been reported in several cancers. However, whether joins Ras-ERK1/2 activation-induced osteosarcoma (OS) cell behaviors remain unclear. The study explored this peradventure and revealed the underlying mechanism.MG-63 cells were transfected with pEGFP-N1, pEGFP-RasWT pEGFP-K-RasG12V/T35S, ERK1/2 expression levels analyzed by Western blot. Effects on viability, migration, colony formation cycle investigated MTT, Transwell,...