Mesenchymal stem cell (MSC) transplantation has been widely applied as a potential therapeutic for multiple diseases. However, the underlying mechanisms are not fully understood, especially paradox between low survival rate of transplanted cells and beneficial effects generated by these cells. Herein, in myocardial infarction (MI) model, we found that MSCs released apoptotic bodies (ABs) to enhance angiogenesis improve cardiac functional reclovery via regulating macroautophagy/autophagy...

Severe hepatic steatosis can exacerbate Ischemia–reperfusion injury (IRI), potentially leading to early graft dysfunction and primary non-function. In this study, we investigated the heterogeneity of different subpopulations Urine-derived stem cells (USCs) explore most suitable cell subtype for treating severe steatotic liver IRI. This study utilized scRNA-seq Bulk RNA-seq investigate transcriptional between Spindle-shaped USCs (SS-USCs) Rice-shaped (RS-USCs). Additionally, rat fatty Liver...

Introduction: Periodontitis is a chronic infectious disease and the major cause of tooth loss other oral health issues around world. Periodontal tissue regeneration has therefore always been ultimate goal dentists researchers. Existing fabrication methods mainly focused on top–down engineering strategy in which several drawbacks remain, including low throughput limited diffusion properties resulting from large sample size. Gelatin methacrylate (GelMA) kind photocrosslinkable biocompatible...

Abstract Severe muscle injury is hard to heal and always results in a poor prognosis. Recent studies found that extracellular vesicle-based therapy has promising prospects for regeneration medicine, however, whether vesicles have therapeutic effects on severe still unknown. Herein, we extracted apoptotic derived from mesenchymal stem cells (MSCs-ApoEVs) treat cardiotoxin induced tibialis anterior (TA) MSCs-ApoEVs promoted muscles increased the proportion of multinucleated cells. Besides...

Abstract Background Sensorineural hearing loss (SNHL) poses a major threat to both physical and mental health; however, there is still lack of effective drugs treat the disease. Recently, novel biological therapies, such as mesenchymal stem cells (MSCs) their products, namely, exosomes, are showing promising therapeutic potential due low immunogenicity, few ethical concerns, easy accessibility. Nevertheless, precise mechanisms underlying effects MSC-derived exosomes remain unclear. Results...

Although stem cell therapy is proved to be a promising strategy for bone repair and regeneration, transplanted allogeneic cells generally suffer from unfavorable apoptosis instead of differentiation into osteocytes. How the apoptotic promote regeneration still needs uncovered. In this work, we found that extracellular vesicles released by are critical mediators promoting regeneration. Based on results in vivo experiments, mechanism determined autologous recruitment enhance osteogenesis was...

Purpose To investigate the involvement of intrinsic mitochondrial apoptosis in dental monomer-induced cytotoxicity and influences N-acetyl cysteine (NAC) on this process. Methods Human pulp cells (hDPCs) were exposed to several monomers absence or presence NAC, cell viability, intracellular redox balance, morphology function mitochondria key indicators evaluated using various commercial kits. Results Dental exerted dose-dependent cytotoxic effects hDPCs. Concomitant over-production reactive...

Abstract: Cell sheet engineering has emerged as a novel approach to effectively deliver seeding cells for tissue regeneration, and developing human bone marrow mesenchymal stem cell (hBMMSC) sheets with high osteogenic ability is constant requirement from clinics faster higher-quality formation. In this work, we fabricated biocompatible safe chitosan (CS)/hyaluronic acid (HA) nanoparticles (NPs) microRNA-21 (miR-21), which been proved accelerate osteogenesis in hBMMSCs; then, the...

Ischemic stroke, a major cause of disability and death worldwide, lacks effective treatments due to the complexity brain ischemia/reperfusion (I/R) injury. The transient receptor potential melastatin 2 (TRPM2) channel is promising therapeutic target. In this study, an extracellular TRPM2 inhibitor A1 with indolizine scaffold was identified through chemical library screening. Four series derivatives were synthesized, yielding four compounds inhibitory activity comparable or superior A1, as...

Polyether-ether-ketone (peek) is one of the most common materials used for load-bearing orthopedic devices owing to its radiolucency and favorable mechanical properties. However, current smooth-surfaced peek implants can lead fibrous capsule formation. To overcome this issue, here, specimens with well-defined internal cross-linked structures (macropore diameters 1.0-2.0 mm) were fabricated using a three-dimensional (3D) printer, an acid-etched microporous surface was achieved...

Vitexin is a natural flavonoid glycoside compound extracted from the leaves and seeds of Vitex negundo. It widely distributed in stems numerous plants exhibites remarkable anti-tumor, anti-inflammatory, anti-hypertensive properties. However, whether vitexin presents anti-aging senescence prevention effect has not been fully elucidated. The purpose this study to investigate on progeria mice cellular senescence, as well its underlying molecular mechanisms. To generate premature...

The reconstruction of bone defects is often impaired by radiotherapy since quality compromised radiation. This study aims to investigate the therapeutic efficacy composite cell sheets-bone marrow mesenchymal stem (BMSC) sheets cocultured with endothelial progenitor cells (EPCs)-in healing irradiated and biological effects EPCs on osteogenic properties BMSC sheets. BMSCs were isolated from rat marrow. used form vitamin C inducing method. seeded make EPCs–BMSC Osteogenesis tested. In vitro...

Irradiated bone has a greater risk of implant failure than nonirradiated bone. The purpose this study was to investigate the influence cell sheets composed co-cultured marrow mesenchymal stromal cells (BMSCs) and endothelial progenitor (EPCs) on osseointegration in irradiated Cell (EPCs, BMSCs or EPCs BMSCs) were wrapped around titanium implants make sheet-implant complexes. group showed highest osteogenic differentiation potential vitro, as indicated by extracellular matrix mineralization...

Abstract Background Under physiological conditions, CXCL12 modulates cell proliferation, survival, angiogenesis, and migration mainly through CXCR4. Interestingly, the newly discovered receptor CXCR7 for is highly expressed in many tumor cells as well tumor-associated blood vessels, although level of normal low. Recently, studies have suggested that promotes growth metastasis more than 20 human malignancies, among which lung cancer leading cause cancer-associated deaths worldwide. Thus,...

Epimedium (EM) and Psoraleae Fructus (PF) are a traditional herb combination often used as fixed form to treat osteoporosis disease in the clinic. However, intricate interactions of this pair remain unknown. In our study, we undertook comprehensive examination their compatibility behaviors. Concurrently, precise sensitive quantitation method was successfully developed validated using liquid chromatography‐tandem mass spectrometry for determination 12 components. This applied analyzing herbal...

7-Ethyl-10-hydroxy-camptothecin (SN38), the active metabolite of irinotecan (CPT-11), is an effective antineoplastic agent against many malignancies. Although it 100-fold to 1000-fold more potent than CPT-11, clinical utility SN38 has been extremely restricted because its poor solubility in any pharmaceutically acceptable solvents. The aim this study was develop nanoparticles (SN38-PC-LNs) using excipients, and investigate therapeutic efficacies vitro vivo. SN38-phospholipid complex...

Post-spaceflight orthostatic intolerance is one of the most important adverse effects after exposure to space microgravity, and there are still no effective countermeasures. It has been considered that arterial remodeling may play an role in occurrence post-spaceflight intolerance, but cellular mechanisms remain unknown. In this study, we investigated whether inflammatory response exists common carotid artery rats exposed simulated microgravity. For this, Sprague-Dawley were subjected 4...

Norathyriol, an aglycone of mangiferin, is a bioactive tetrahydroxyxanthone present in mangosteen and many medicinal plants. However, the biological fate norathyriol vivo remains unclear. In this study, absorption metabolism rats were evaluated through HPLC-MS/MS. Results showed that was well absorbed, as indicated by its absolute bioavailability 30.4%. Besides, total 21 metabolites identified rats, including methylated, glucuronidated, sulfated glycosylated conjugates, which suggested...

Dental resin monomers such as 2-hydroxyethyl methacrylate (HEMA) disturb vital cell functions and induce mitochondrial intrinsic apoptosis via generation of oxidative stress. Nuclear factor erythroid 2-related 2 (Nrf2) regulates the gene expression antioxidative enzymes plays a crucial role in maintenance cellular redox equilibrium homeostasis. The present study investigated functional significance Nrf2 response toward HEMA. It was found that HEMA stimulation promoted nuclear translocation...

1. The aim of the present study was to investigate effects simulated microgravity on arterial dilatory responsiveness and L-arginine (L-Arg)-nitric oxide (NO)-cGMP pathway in abdominal aorta rats. 2. Twenty healthy male Sprague-Dawley were randomly divided into control groups. Rats group subjected hindlimb unweighting (HU). After 4 weeks, examined vitro isolated aortic rings. Western blotting used measure endothelial (e) inducible (i) NO synthase (NOS) protein content. Total concentrations...

Microgravity-induced vascular remodelling may play an important role in post-spaceflight orthostatic intolerance. In this study, we aimed to investigate the effects of simulated microgravity on monocyte adhesion aortic endothelium hindlimb unweighted rats and elucidate underlying mechanisms associated with event. Sprague-Dawley were subjected 4-week unweighting simulate microgravity. The recruitment monocytes abdominal aorta was investigated by en face immunofluorescence staining binding...

Recent studies have suggested that microgravity-induced arterial remodelling contributes to post-flight orthostatic intolerance and multiple mechanisms are involved in remodelling. However, the initial mechanism by which haemodynamic changes induce is unknown. Focal adhesions (FAs) dynamic protein complexes mechanotransduction properties. This study aimed investigate role of FAs simulated-microgravity-induced basilar femoral A 4-week hindlimb-unweighted (HU) rat model was used simulate...