A unique feature of the pathological change after spinal cord injury (SCI) is progressive enlargement lesion area, which usually results in cavity formation and accompanied by reactive astrogliosis chronic inflammation. Reactive astrocytes line cavity, walling off core from normal tissue, are thought to play multiple important roles SCI. The contribution cell death, particularly apoptosis neurons oligodendrocytes during process cavitation has been extensively studied. However, how eliminated...

Abstract Background Oligodendrocytes (OLs) death after spinal cord injury (SCI) contributes to demyelination, even leading a permanent neurological deficit. Besides apoptosis, our previous study demonstrated that OLs underwent receptor-interacting serine-threonine kinase 3(RIP3)/mixed lineage domain-like protein (MLKL)-mediated necroptosis. Considering necroptosis is always accompanied with pro-inflammatory response and quercetin has long been used as anti-inflammatory agent, in the present...

Purpose To investigate the involvement of intrinsic mitochondrial apoptosis in dental monomer-induced cytotoxicity and influences N-acetyl cysteine (NAC) on this process. Methods Human pulp cells (hDPCs) were exposed to several monomers absence or presence NAC, cell viability, intracellular redox balance, morphology function mitochondria key indicators evaluated using various commercial kits. Results Dental exerted dose-dependent cytotoxic effects hDPCs. Concomitant over-production reactive...

In worldwide, osteoporosis has become one of the severe public health distress and over 200 million people get affected by tenderness fissure during their life period. Vicenin-2 is a naturally occurring flavonoid glycoside present in Moringa oleifera, Peperomia blanda Ocimum sanctum Linn with numerous biological activities. The study aims to assess effect on ovariectomy-induced postmenopausal female rats. Surgical removal ovaries was achieved institute ovariectomy animal model....

Transplantation of olfactory ensheathing cells (OECs) has been demonstrated to be beneficial for spinal cord injury (SCI) by modulating neuroinflammation, supporting neuronal survival and promoting angiogenesis. Besides OECs, the conditioned medium (CM) from OECs also proved have therapeutic effects SCI, indicating that bioactive substances secreted are essential its protective effects. Nevertheless, there is still little information regarding underlying mechanisms. Considering exosomes...

Abstract Background Hydroxysafflor Yellow A (HSYA), which is one of the most important active ingredients Chinese herb Carthamus tinctorius L , widely used in treatment cerebrovascular and cardiovascular diseases. However, potential protective effect HSYA spinal cord ischemia/reperfusion (I/R) injury still unknown. Methods Thirty-nine rabbits were randomly divided into three groups: sham group, I/R group group. All animals sacrificed after neurological evaluation with modified Tarlov...

Background: Patients undergoing posterior spinal instrumentation and fusion surgery for adolescent idiopathic scoliosis were admitted to the intensive care unit until two years ago, at which time we changed our protocol admit these patients general hospital floor following a brief stay in postanesthesia unit. This study compared postoperative management on with that fusion. Methods: A retrospective review of 124 consecutive treated from August 2007 2010 was performed. Inclusion criteria...

Background context Low back pain, affecting nearly 40% of adults, mainly results from intervertebral disc degeneration (IVDD), while the pathogenesis IVDD is still not fully elucidated. Recently, some researches have revealed that necroptosis, a programmed necrosis, participated in progression IVDD, nevertheless, underlying mechanism remains unclear. Purpose To study necroptosis Nucleus Pulposus (NP) cells focusing on role MyD88 signaling. Study design The expression and co-localization...

Abstract The purpose of this study is to compare the accuracy and effectiveness ultrasound-guided fluoroscopy-guided lumbar selective nerve root block (SNRB), explore feasibility methods. This retrospective included patients with radicular pain who underwent at Honghui Hospital Affiliated Xi’an Jiaotong University from August 2020 2022. Patients were divided into U-SNRB group F-SNRB according or block. There 43 in 20 group. visual analogue scale (VAS) scores, Japanese Orthopaedic Association...

Abstract Background Glioblastoma (GBM) is a high-grade and heterogeneous subtype of glioma that presents substantial challenge to human health, characterized by poor prognosis low survival rates. Despite its known involvement in regulating leukemia melanoma, the function mechanism DNAJC1 GBM remain poorly understood. Methods Utilizing data from TCGA, CGGA, GEO databases, we investigated expression pattern correlation with clinical characteristics specimens. Loss-of-function experiments were...

Objectives. This study aimed to investigate the influences of N-acetyl cysteine (NAC) on cytotoxicity and mechanical properties Poly-methylmethacrylate (PMMA) dental resins. Methods. Experimental PMMA resin was prepared by incorporating various concentrations NAC (0, 0.15, 0.3, 0.6 0.9 wt.%). MTT assay performed viability human pulp cells after exposure extract with or without NAC. Cell adhesion specimens examined scanning electron microscopy. Degree conversion studied Fourier Transform...

Abstract Background Osteosarcoma is the most common primary bone cancer occurring in young adults and 5-year survival rate of patients with metastatic osteosarcoma less than 30% due to high recurrence drug resistance. Notch a highly conserved cell signaling pathway evolution, Jagged1 an important ligand Notch. Although some studies have found that receptors ligands including were expressed tissues cells, role progression metastasis are still not clear. Methods Tumor collected from 68...

Methacryloxylethyl cetyl ammonium chloride (DMAE-CB) is a polymerizable antibacterial monomer and has been proved as an effective strategy to achieve bioactive bonding with reliable bacterial inhibitory effects. However, the toxicity of DMAE-CB may hamper its wide application in clinical situations. Thus, this study was designed investigate explore possible protective effects N-acetyl cysteine (NAC). High performance liquid chromatography (HPLC) chromatography-mass spectrometry (LC-MS)...

Notch signaling plays a vital role in tumorigenicity and tumor progression by regulating proliferation, invasion, the microenvironment. Previous research our group indicated that ligand Delta-like 1 (Dll1) is involved angiogenesis melanoma, we noticed it took longer time to trypsinize Dll1-expressing B16 melanoma cells than control cells. In this article, extended study investigate effects of Dll1 on cell adhesion metastasis. overexpression activated significantly enhanced adhering capacity...

BACKGROUND:Pedicle screw fixation is one of the most commonly used methods in spine surgery. We introduce a surgical robot system from China based on 3-dimensional fluoroscopy imaging and compare it with O-arm navigation system. study differences accuracy, safety, clinical effect auxiliary pedicle fixation. MATERIAL AND METHODS:Patients who underwent thoracolumbar internal our hospital 2017 to 2019 were divided into group according whether surgery was assisted by Tinavi orthopedic or Imaging...

Resin monomers from dental composite materials leached due to incomplete polymerization or biodegradation may cause contact allergies and damage pulp. The cytotoxicity of resin is a disturbance intracellular redox equilibrium, characterized by an overproduction reactive oxygen species (ROS) depletion reduced glutathione (GSH). Oxidative stress caused leads the vital cell functions induction apoptosis in affected cells. nuclear factor-erythroid 2-related factor 2 (Nrf2) pathway plays key role...

The transforming growth factor beta (TGF-β) signaling pathway is believed to play essential roles in several physiological activities, including cancer. TGF-β receptor type I (TBR-I) a key membrane protein the pathway, which relates many intracellular biological effects. In recent years, cold atmospheric plasma (CAP) has been found have promising prospects selective anticancer therapy and confirmed its role pathway. However, ambiguous effect of CAP-induced electric field (EF) on TBR-I still...

BackgroundGlioma, a prevalent malignancy of the brain and spinal cord, poses considerable threat to human health. The association between aberrant sialic acid modification glioma progression has been suggested, but precise mechanism is still elusive. ST3GAL4, sialoglycosyltransferase, implicated in increased metastatic potential poor prognosis various cancers; however, its specific role requires further elucidation.MethodsWe evaluated ST3GAL4 expression levels their clinical relevance using...

Robotic-assisted surgery has emerged as an innovative approach widely adopted in the field of orthopedics. However, its application specifically for managing atlantoaxial dislocation with a high-riding vertebral artery (AAD-HVA) remains underreported existing literature.

Antibacterial monomers incorporated in dentin bonding systems may have toxic effects on the pulp.Thus, cytotoxicity of antibacterial and its underlying mechanisms must be elucidated to improve safety monomer application.The influence an monomer, methacryloxylethyl cetyl ammonium chloride (DMAE-CB), vitality L929 mouse fibroblasts was tested using MTT assay.Cell cycle progression studied flow cytometry.Production intracellular reactive oxygen species (ROS) after DMAE-CB treatment measured...

<i >Background</i>. The B2-bradykinin receptor (BDKRB2) has been reported to associate with onset and development of Osteoarthritis (OA); however, the role BDKRB2 genetic polymorphisms in OA remains unknown. >Method</i>. A total 245 patients primary knee 264 healthy volunteer were recruited. gene polymorphisms, &#x2212;58T/C +9/&#x2212;9&#x2009;bp genotyped. >Results</i>. genotype distributions allele frequencies significantly differed between control subjects. Logistic regression analysis...

Abstract The aim of this study was to evaluate the efficacy and feasibility a life-size 3-dimensional printing assisted posterior internal fixation. We performed retrospective review 138 patients who received atlantoaxial fixation from October 2009 March 2015 with minimum follow-up period 12 months. Group A included 76 conventional free-hand technique. B 62 were treated by 3D printing. placement accuracy screw evaluated in computed tomography images according methods Hojo clinical outcomes...

The polymerizable antibacterial monomer methacryloxylethyl cetyl ammonium chloride (DMAE-CB) has provided an effective strategy to combat dental caries.However, the application of such material raises question about biological safety and remains open.The mechanism this toxic action, however, is not yet clearly understood.The present study aims at providing novel insight into possible causal link between cellular oxidative stress DNA damage, as well apoptosis in human pulp cells exposed...