Non- small- cell lung cancer (NSCLC) is one of the most leading causes cancer-related deaths worldwide. Paclitaxel based combination therapies have long been used as a standard treatment in aggressive NSCLCs. But paclitaxel resistance has emerged major clinical problem combating non-small-cell and autophagy important mechanisms involved this phenomenon. In study, we microRNA (miRNA) arrays to screen differentially expressed miRNAs between sensitive cells A549 its paclitaxel-resistant variant...

The effect of gold nanoparticles (AuNPs) on the polymerization tubulin has not been examined till now. We report that interaction weakly protected AuNPs with microtubules (MTs) could cause inhibition and aggregation in cell free system. estimate single citrate capped ∼105 heterodimers. Investigation nature by Raman Fourier transform-infrared (FTIR) spectroscopies indicated partial conformational changes microtubules, thus revealing AuNP-induced change is driving force behind observed...

Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone), a naphthoquinone isolated from the roots of Plumbaginaceae plants, has potential antiproliferative activity against several tumor types. We have examined effects plumbagin on cellular microtubules ex vivo as well its binding with purified tubulin and in vitro. Cell viability experiments using human non-small lung epithelium carcinoma cells (A549) indicated that IC50 value for is 14.6 μM. Immunofluorescence studies an antitubulin FITC...

The biological significance of microtubules makes them a validated target cancer therapy. In this study, we have utilized indole, an important pharmacological scaffold, to synthesize novel bis(indolyl)-hydrazide–hydrazone derivatives (NMK-BH compounds) and recognized NMK-BH3 as the most effective one in inhibiting A549 cell proliferation assembly tissue-purified tubulin. Cell viability experiments showed that inhibited human lung adenocarcinoma (A549) cells, normal fibroblasts (WI38)...

Metformin, a widely prescribed anti-diabetic drug, shows anticancer activity in various cancer types. Few studies documented that there was decreased level of LDL and total cholesterol blood serum metformin users. Based on these views, this study aimed to determine if exhibits by alleviating cells. The present found treatment breast MDA-MB-231 cells with significantly content concomitant inhibition regulatory genes (e.g., HMGCoR, LDLR SREBP1). Metformin cell viability, migration stemness...

Parabenzoquinone (1,4-benzoquinone) (PBQ) is a bioactve quinone present in cigarette smoke and diesel smoke, which causes severe genotoxic effects both vitro vivo. In the previous study, we showed that microtubules are one of major targets smoke-induced damage lung epithelium cells. have investigated effect PBQ on cellular using human type II epithelial cells (A549) also purified tubulin. Cell viability experiments A549 indicated very low IC(50) value (approximately 7.5 microM) for PBQ....

2,4-Dichlorophenoxyacetic acid (2,4-D), the most widely used herbicide in world, has been previously reported to induce lung damage. Here this study we have investigated molecular mechanism of 2,4-D induced toxicity A549 and WI38 cell lines. Cell viability experiments indicate IC50 values cells for 72 h are 126 ± 2.25 μM 115 4.39 μM, respectively. Although not arresting a particular phase cycle cells, dose dependently increased subG1 population, indicative death, mode death is apoptosis both...

Colchicine is a well-known and potent microtubule targeting agent, but the therapeutic value of colchicine against cancer limited by its toxicity normal cells. But, there no report cytotoxic potential lung cell, at clinically permissible or lower concentrations, minimally toxic to non-cancerous Hence, in present study, we investigated possible mechanism which efficacy cells less dose could be enhanced. admissible concentration 2.5 nM had effect caused G2/M arrest A549 However, this...

In response to rising concerns about the environmental impact of standard chemical synthesis methods, Erythrina variegata leaf extract facilitated green CuO NPs for potent anticancer, antibacterial, and photocatalytic applications.

Deuterium oxide (D(2)O) is known to promote the assembly of tubulin into microtubules in vitro, increase volume mitotic spindles and number length spindle microtubules, inhibit mitosis. Reasoning that its actions on cellular could be due modulation microtubule dynamics, we examined effects replacing H(2)O with D(2)O dynamic instability, treadmilling, steady-state GTPase activity. We found 50% or more promoted polymerization stabilized against dilution-induced disassembly. Using...

Tubulin is an unstable protein when stored in solution and loses its ability to form microtubules rapidly. We have found that D2O stabilizes the against inactivation at both 4 37 °C. In H2O-based buffer, tubulin was completely inactivated after 40 h °C, but buffer prepared D2O, no activity lost 54 h. °C 8 H2O only 20% of buffer. also colchicine binding a slower rate D2O. The deuterated solvent retarded aggregation process occurs during incubation temperatures. Inactivation partially reversed...

Genistein (4',5,7-trihydroxyisoflavone), an isoflavone, is a major constituent of soyfoods. It has potential antiproliferative activity against several tumor types. We have examined the effect genistein on cellular microtubules as well its binding with purified tubulin in vitro. Cell viability experiments using human non-small lung epithelium carcinoma cells (A549) indicated that IC(50) value for 72 microM. Flow cytometry demonstrated arrested cell cycle progression at G(2)/M phase, but...

Paclitaxel (Tx) is one of the first-line chemotherapeutic drugs used against lung cancer, but acquired resistance to this drug a major challenge successful chemotherapy. In work, we have focused on chronological changes various cellular parameters and associated effect Tx (10 nM) development in A549 cell line. It was observed, at initial stage, death percentage due treatment had increased up 20 days, thereafter, it started declining became completely resistant by 40 days. Expressions βIII...