A5070806363- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Immune Cell Function and Interaction
- Biopolymer Synthesis and Applications
- Cancer Research and Treatments
- T-cell and B-cell Immunology
- Retinal Diseases and Treatments
- DNA Repair Mechanisms
- Nitric Oxide and Endothelin Effects
- Cardiac Structural Anomalies and Repair
- Ocular and Laser Science Research
- Cellular transport and secretion
- Virus-based gene therapy research
- Retinal and Macular Surgery
- Polyamine Metabolism and Applications
- Drug-Induced Ocular Toxicity
- Glycosylation and Glycoproteins Research
- Retinoids in leukemia and cellular processes
- Epigenetics and DNA Methylation
- Intraocular Surgery and Lenses
- Molecular Biology Techniques and Applications
- Bacterial Genetics and Biotechnology
- Cytomegalovirus and herpesvirus research
- Cervical Cancer and HPV Research
- Mechanisms of cancer metastasis
Illinois Institute of Technology
2023
National Institutes of Health
2017-2023
National Heart Lung and Blood Institute
2017-2023
Hubei University
2018-2019
Harvard University
2007-2019
University of Science and Technology of China
2016-2019
Center for Excellence in Molecular Cell Science
2016-2019
Jiangsu University
2018
Smith-Kettlewell Eye Research Institute
2007-2010
Cytokines critically control immune responses, but how regulatory programs are altered to allow T cells differentially respond distinct cytokine stimuli remains poorly understood. Here, we have globally analyzed enhancer elements bound by IL-2-activated STAT5 and IL-21-activated STAT3 in identified Il2ra as the top-ranked gene regulated an STAT5-bound superenhancer one of top genes STAT3-bound superenhancers. Moreover, found that binding was rapidly superenriched at highly induced IL-2...
Abstract Poly‐γ‐glutamic acid (γ‐PGA) is an important multifunctional biopolymer with various applications, for which adenosine triphosphate (ATP) supply plays a vital role in biosynthesis. In this study, the enhancement of γ‐PGA production was attempted through approaches improving ATP engineered strains Bacillus licheniformis . The first approach to engineer respiration chain branches B. , elimination cytochrome bd oxidase branch reduced maintenance coefficient, leading 19.27% increase...
Interleukin-2 is a pleiotropic cytokine that mediates both pro- and anti-inflammatory functions. Immune cells naturally differ in their sensitivity to IL-2 due cell type activation state-dependent expression of receptors signaling pathway components. To probe differences across types, we used structure-based design create profile series variants with the capacity titrate maximum signal strength fine increments. One these partial agonists, IL-2-REH, specifically expanded Foxp3+ regulatory T...
Faithful segregation of mitotic chromosomes requires bi-orientation sister chromatids, which relies on the sensing correct attachments between spindle microtubules and kinetochores. Although mechanisms underlying PLK1 activation have been extensively studied, regulatory that couple activity to accurate chromosome are not well understood. In particular, is implicated in stabilizing kinetochore-microtubule attachments, but how kinetochore regulated avoid hyperstabilized...
Heterochromatin protein 1α (HP1α) regulates chromatin specification and plasticity during cell fate decision. Different structural determinants account for HP1α localization function division cycle. Our earlier study showed that centromeric of depends on the epigenetic mark H3K9me3 in interphase, while its location mitosis relies uncharacterized PXVXL-containing factors. Here, we identified a protein, ligand-dependent nuclear receptor-interacting factor 1 (LRIF1), which recruits to...
Cell migration is orchestrated by dynamic interactions of microtubules with the plasma membrane cortex. How these facilitate processes still being actively investigated. TIP150 a newly characterized microtubule plus end tracking protein essential for mitosis and entosis (Ward, T., Wang, M., Liu, X., Z., Xia, P., Chu, Y., L., Jiang, K., Yu, H., Yan, J., Hill, D. Huang, Zhu, Yao, X. (2013) Regulation interaction between two microtubule-binding proteins, EB1 TIP150, mitotic p300/CBP-associated...
<h2>Abstract</h2><h3>Background</h3> We have developed a tissue engineered cardiac patch derived from 3-dimensional (3D) myocardial reinforced with extracellular matrix in an effort to enhance situ regeneration. The feasibility of the was evaluated porcine model by various modalities assess both constructive and functional aspects <h3>Methods</h3> A spheroid-based 3D multicellular created using net mold system that incorporated cardiomyocytes embryonic fibroblast cells. sheets surgically...
Chromosome segregation in mitosis is orchestrated by the dynamic interactions between kinetochore and spindle microtubules. Our recent study shows SKAP an EB1-dependent, microtubule plus-end tracking protein essential for oscillations during mitosis. Here we show that phosphorylation of GSK3β regulates Kif2b depolymerase activity competing binding. a bona fide substrate vitro accurate kinetochore-microtubule attachment cells. The GSK3β-elicited sites were mapped mass spectrometry...
Abstract Somatic mutations can lead to the transformation of healthy cells into malignant and allow their evasion from immune surveillance. To uncover genes that play a role in detection lysis tumor by natural killer (NK) cells, B lymphoblastoid cell line was subjected genome-wide CRISPR screen. Among top hits facilitated NK SPPL3 , which encodes an intramembrane protease cleaves transmembrane glycosyltransferases Golgi apparatus. -deficient accumulated glycosyltransferases, such as...
Chromosome movements during mitosis are orchestrated primarily by the interaction of spindle microtubules with kinetochore, site for attachment to centromere, at which mitotic kinase circuitry synergized ensure quality control cell division. Mitotic master Cdk1 controls many aspects chromosome behavior and microtubule dynamics accurate segregation. Polo 1 (PLK1) is a highly conserved activation PLK1 requires phosphorylation T‐loop. Here we show that T‐loop CDK1 in Thr214 essential temporal...
Abstract Poly-γ-glutamic acid (γ-PGA) is a multifunctional and naturally occurring biopolymer made from D- L-glutamate as monomers, which mainly produced by Bacillus . Few reports have been focused on the regulation network of γ-PGA synthesis in recent years. In this study, we demonstrated that licheniformis global nitrogen homeostatic regulator TnrA direct repressor synthase PgsBCAA synthesis. First, our results confirmed repressed synthesis, deficiency tnrA led to 22.03% increase...
Six genome sequences of papillomavirus were determined from oral and skin swabs fecal samples collected wild rats. Three genomes 7,722 bp, two 7,716 one was 7,730 displaying typical organizations.